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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
17/01/2002 |
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Data da última atualização: |
08/05/2023 |
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Autoria: |
BERCKER, D. K.; DYGDALE, B.; SMITH, M. K.; HARDING, R. M.; DALE, J. L. |
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Título: |
Genetic transformation of cavendish banana (Musa spp. AAA group) cv Grand Nain via microprojectile bombardment. |
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Ano de publicação: |
2000 |
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Fonte/Imprenta: |
Plant Cell Reports, v.19, n.3, p.229-234, 2000. |
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Idioma: |
Inglês |
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Conteúdo: |
An effective method has been developed for the stable transformation and regeneration of Cavendish banana (Musa spp. AAA group) cv Grand Nain by microprojectole bombardment. Embryogenic cell suspensions were initiated using immature male flowers as the explant. Cells were co-bombarded with the neomycin phosphotransferase (nptII) selectable marker gene under the control of a banana bunchy top virus (BBTV) promoter or the CaMV 35S promoter, and either the B-glucuronidase (uidA) reporter gene or BBTV genes under the control of the maize polyubiquitin promoter. |
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Palavras-Chave: |
Microprojectile bombardment; Transformation. |
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Thesagro: |
Banana. |
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Thesaurus Nal: |
Musa. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01204naa a2200217 a 4500
001 1650368
005 2023-05-08
008 2000 bl uuuu u00u1 u #d
100 1 $aBERCKER, D. K.
245 $aGenetic transformation of cavendish banana (Musa spp. AAA group) cv Grand Nain via microprojectile bombardment.$h[electronic resource]
260 $c2000
520 $aAn effective method has been developed for the stable transformation and regeneration of Cavendish banana (Musa spp. AAA group) cv Grand Nain by microprojectole bombardment. Embryogenic cell suspensions were initiated using immature male flowers as the explant. Cells were co-bombarded with the neomycin phosphotransferase (nptII) selectable marker gene under the control of a banana bunchy top virus (BBTV) promoter or the CaMV 35S promoter, and either the B-glucuronidase (uidA) reporter gene or BBTV genes under the control of the maize polyubiquitin promoter.
650 $aMusa
650 $aBanana
653 $aMicroprojectile bombardment
653 $aTransformation
700 1 $aDYGDALE, B.
700 1 $aSMITH, M. K.
700 1 $aHARDING, R. M.
700 1 $aDALE, J. L.
773 $tPlant Cell Reports$gv.19, n.3, p.229-234, 2000.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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