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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
07/04/2000 |
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Data da última atualização: |
10/05/2023 |
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Autoria: |
SARRIA, R.; TORRES, E.; ANGEL, F.; CHAVARRIAGA, P.; ROCA, W. M. |
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Título: |
Transgenic plants of cassava (Manihot esculenta) with resistance to Basta obtained by Agrobacterium-mediated transformation. |
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Ano de publicação: |
2000 |
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Fonte/Imprenta: |
Plant Cell Reports, v.19, p.339-344, 2000. |
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Idioma: |
Inglês |
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Conteúdo: |
Transgenic plants of cassava (Manihot esculenta) resistant to the herbicide Basta were obtained through Agrobacterium-mediated transformation. The plants also expressed the uidA gene and two were positive forPCR- and/or Sourthern-based detection of the nptII gene. Somatic-embryo-derived cotyledons were used as source of explants. A non-disarmed Agrobacterium strain (CIAT 1182) was used to transfer the genes of interest into cassava cultivar MPer183. Greenhouse tests of resistance to Basta (Hoechst) showed three plant lines with different levels of tolerance to the herbicide. Based on Southern tests of transgenesis, the transformation efficiency was 1%. The results constitute the first report of the bar gene conferring herbicide resistance to cassava plants. |
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Palavras-Chave: |
ppt/Basta-resistence; transformation; Useful transgenes. |
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Thesagro: |
Manihot Esculenta. |
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Thesaurus Nal: |
Agrobacterium; cassava; transgenic plants. |
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Categoria do assunto: |
-- |
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Marc: |
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100 1 $aSARRIA, R.
245 $aTransgenic plants of cassava (Manihot esculenta) with resistance to Basta obtained by Agrobacterium-mediated transformation.$h[electronic resource]
260 $c2000
520 $aTransgenic plants of cassava (Manihot esculenta) resistant to the herbicide Basta were obtained through Agrobacterium-mediated transformation. The plants also expressed the uidA gene and two were positive forPCR- and/or Sourthern-based detection of the nptII gene. Somatic-embryo-derived cotyledons were used as source of explants. A non-disarmed Agrobacterium strain (CIAT 1182) was used to transfer the genes of interest into cassava cultivar MPer183. Greenhouse tests of resistance to Basta (Hoechst) showed three plant lines with different levels of tolerance to the herbicide. Based on Southern tests of transgenesis, the transformation efficiency was 1%. The results constitute the first report of the bar gene conferring herbicide resistance to cassava plants.
650 $aAgrobacterium
650 $acassava
650 $atransgenic plants
650 $aManihot Esculenta
653 $appt/Basta-resistence
653 $atransformation
653 $aUseful transgenes
700 1 $aTORRES, E.
700 1 $aANGEL, F.
700 1 $aCHAVARRIAGA, P.
700 1 $aROCA, W. M.
773 $tPlant Cell Reports$gv.19, p.339-344, 2000.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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| 1. |  | ARAUJO, Q. R. de; GATTWARD, J. N.; DANTAS, P. A.; RIBEIRO, D. O.; SANTOS, T. L.; SANTOS, L. C.; CARVALHO JUNIOR, J. C. S.; MANZ, J.; CHEPOTE, R. E.; SOUZA, L. S. Propriedades de um solo coeso de Tabuleiro por efeito da latoção de pastagem consorciada. In: REUNIÃO BRASILEIRA DE MANEJO E CONSERVAÇÃO DO SOLO E DA ÁGUA. 18., 2010, Teresina. Novos caminhos para a agricultura conservacionista no Brasil. Teresina: Embrapa Meio Norte, 2010. 1 CD-ROM. PDF 30.| Tipo: Artigo em Anais de Congresso |
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