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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
27/10/1994 |
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Data da última atualização: |
05/10/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ARAUJO, R. S.; MAYA-FLORES, J.; BARNES-McCONNELL, D.; YOKOYAMA, C.; DAZZO, F. B.; BLISS, F. A. |
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Afiliação: |
RICARDO SILVA ARAUJO, CNPAF; JAIME MAYA-FLORES; DEBORAH BARNES-MCCONNELL; CHARLES YOKOYAMA; FRANK B. DAZZO; FREDRICK A. BLISS. |
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Título: |
Semienclosed tube cultures of bean plants (Phaseolus vulgaris L.) for enumeration of Rhizobium phaseoli by the most-probable-number technique. |
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Ano de publicação: |
1986 |
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Fonte/Imprenta: |
Applied and Environmental Microbiology, v. 52, n. 4, p.954-956, Oct. 1986. |
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Idioma: |
Inglês |
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Conteúdo: |
The semienclosed tube culture technique of Gibson was modified to permit growth of common bean (Phaseolus vulgaris L.) roots in humid air, enabling enumeration of the homologous (nodule forming) symbiont, Rhizobium phaseoli, by the most-probable-number plant infection method. A bean genotype with improved nodulation characteristics was used as the plant host. This method of enumeration was accurate when tubes were scored 3 weeks after inoculation with several R. phaseoli strains diluted from aqueous suspensions, peat-based inoculants, or soil. A comparison of population sizes obtained by most-probable-number tubecultures and plate counts indicated that 1 to 3 viable cells of R. phaseoli were a sufficient inoculant to induce nodule formation. |
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Palavras-Chave: |
Tube culture. |
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Thesagro: |
Feijão; Phaseolus Vulgaris; Rhizobium. |
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Thesaurus Nal: |
Rhizobium phaseoli; Root inoculation. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 01529naa a2200253 a 4500
001 1197811
005 2021-10-05
008 1986 bl uuuu u00u1 u #d
100 1 $aARAUJO, R. S.
245 $aSemienclosed tube cultures of bean plants (Phaseolus vulgaris L.) for enumeration of Rhizobium phaseoli by the most-probable-number technique.
260 $c1986
520 $aThe semienclosed tube culture technique of Gibson was modified to permit growth of common bean (Phaseolus vulgaris L.) roots in humid air, enabling enumeration of the homologous (nodule forming) symbiont, Rhizobium phaseoli, by the most-probable-number plant infection method. A bean genotype with improved nodulation characteristics was used as the plant host. This method of enumeration was accurate when tubes were scored 3 weeks after inoculation with several R. phaseoli strains diluted from aqueous suspensions, peat-based inoculants, or soil. A comparison of population sizes obtained by most-probable-number tubecultures and plate counts indicated that 1 to 3 viable cells of R. phaseoli were a sufficient inoculant to induce nodule formation.
650 $aRhizobium phaseoli
650 $aRoot inoculation
650 $aFeijão
650 $aPhaseolus Vulgaris
650 $aRhizobium
653 $aTube culture
700 1 $aMAYA-FLORES, J.
700 1 $aBARNES-McCONNELL, D.
700 1 $aYOKOYAMA, C.
700 1 $aDAZZO, F. B.
700 1 $aBLISS, F. A.
773 $tApplied and Environmental Microbiology$gv. 52, n. 4, p.954-956, Oct. 1986.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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