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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
03/09/2024 |
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Data da última atualização: |
03/09/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MAGALHÃES, L. O.; SOUZA, U.; RAIOL‑JÚNIOR, L. L.; CARVALHO, E. V. de; MOREIRA, A. S.; ASTUA, J. de F.; GIRARDI, E. A.; GESTEIRA, A. da S. |
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Afiliação: |
LEANDRA OLIVEIRA MAGALHÃES, SANTA CRUZ STATE UNIVERSITY; UIARA SOUZA, SANTA CRUZ STATE UNIVERSITY; LAUDECIR LEMOS RAIOL‑JÚNIOR; EVERTON VIEIRA DE CARVALHO, HELIX SEEDS AND BIOTECHNOLOGY; ALECIO SOUZA MOREIRA, CNPMF; JULIANA DE FREITAS ASTUA, CNPMF; EDUARDO AUGUSTO GIRARDI, CNPMF; ABELMON DA SILVA GESTEIRA, CNPMF. |
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Título: |
Antibiotics and thermotherapy have limited effectiveness in eliminating Candidatus Liberibacter asiaticus from citrus budwood. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
European Journal of Plant Pathology, August 2024. |
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ISSN: |
0929-1873 |
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DOI: |
https://doi.org/10.1007/s10658-024-02939-5 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract Epigenetic markers related to resistance require regenerating healthy trees from previously infected plant tissues. Thus, sanitizing and regenerating healthy citrus plants from previously Candidatus Liberibacter asiaticus (CLas)-infected tissues constitute a valuable need for breeding programs. Immersion in ampicillin + streptomycin solution and thermotherapy were evaluated separately for eliminating CLas from infected citrus propagules. Valencia sweet orange (Citrus × sinensis) and trifoliate orange (Citrus trifoliata) were chosen as genotypes that were highly and moderately susceptible to CLas, respectively. Infected budwood was used to graft nucellar material with or without CLas. Thirty-two months after inoculation, 2.5-cm grafts were collected and treated by 12 h immersion in an antibiotic solution (1 g/L ampicillin + 0.1 g/L streptomycin) or in autoclaved Milli-Q water, and subsequently grafted onto Rangpur lime (C. × limonia) rootstocks. In a second experiment, thermotherapy was evaluated for treating stem cuttings kept for 0, 1.5 and 10 min at 55 °C in a water bath. The antibiotics did not influence plant regeneration, but CLas was effectively eliminated from only 22.2% of the infected propagules of trifoliate orange up to 15 months after treatment. Thermotherapy at 55 °C for 1.5 min was ineffective in completely suppressing CLas from trifoliate orange plants eight months after treatment, and was harmful to citrus propagation, notably sweet orange. Antibiotic treatment did not eliminate CLas in sweet orange propagules, but a decrease in titer was recorded for both genotypes. MenosAbstract Epigenetic markers related to resistance require regenerating healthy trees from previously infected plant tissues. Thus, sanitizing and regenerating healthy citrus plants from previously Candidatus Liberibacter asiaticus (CLas)-infected tissues constitute a valuable need for breeding programs. Immersion in ampicillin + streptomycin solution and thermotherapy were evaluated separately for eliminating CLas from infected citrus propagules. Valencia sweet orange (Citrus × sinensis) and trifoliate orange (Citrus trifoliata) were chosen as genotypes that were highly and moderately susceptible to CLas, respectively. Infected budwood was used to graft nucellar material with or without CLas. Thirty-two months after inoculation, 2.5-cm grafts were collected and treated by 12 h immersion in an antibiotic solution (1 g/L ampicillin + 0.1 g/L streptomycin) or in autoclaved Milli-Q water, and subsequently grafted onto Rangpur lime (C. × limonia) rootstocks. In a second experiment, thermotherapy was evaluated for treating stem cuttings kept for 0, 1.5 and 10 min at 55 °C in a water bath. The antibiotics did not influence plant regeneration, but CLas was effectively eliminated from only 22.2% of the infected propagules of trifoliate orange up to 15 months after treatment. Thermotherapy at 55 °C for 1.5 min was ineffective in completely suppressing CLas from trifoliate orange plants eight months after treatment, and was harmful to citrus propagation, notably sweet orange. Antibioti... Mostrar Tudo |
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Thesaurus Nal: |
Ampicillin; Citrus; Streptomycin. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02462naa a2200265 a 4500
001 2167075
005 2024-09-03
008 2024 bl uuuu u00u1 u #d
022 $a0929-1873
024 7 $ahttps://doi.org/10.1007/s10658-024-02939-5$2DOI
100 1 $aMAGALHÃES, L. O.
245 $aAntibiotics and thermotherapy have limited effectiveness in eliminating Candidatus Liberibacter asiaticus from citrus budwood.$h[electronic resource]
260 $c2024
520 $aAbstract Epigenetic markers related to resistance require regenerating healthy trees from previously infected plant tissues. Thus, sanitizing and regenerating healthy citrus plants from previously Candidatus Liberibacter asiaticus (CLas)-infected tissues constitute a valuable need for breeding programs. Immersion in ampicillin + streptomycin solution and thermotherapy were evaluated separately for eliminating CLas from infected citrus propagules. Valencia sweet orange (Citrus × sinensis) and trifoliate orange (Citrus trifoliata) were chosen as genotypes that were highly and moderately susceptible to CLas, respectively. Infected budwood was used to graft nucellar material with or without CLas. Thirty-two months after inoculation, 2.5-cm grafts were collected and treated by 12 h immersion in an antibiotic solution (1 g/L ampicillin + 0.1 g/L streptomycin) or in autoclaved Milli-Q water, and subsequently grafted onto Rangpur lime (C. × limonia) rootstocks. In a second experiment, thermotherapy was evaluated for treating stem cuttings kept for 0, 1.5 and 10 min at 55 °C in a water bath. The antibiotics did not influence plant regeneration, but CLas was effectively eliminated from only 22.2% of the infected propagules of trifoliate orange up to 15 months after treatment. Thermotherapy at 55 °C for 1.5 min was ineffective in completely suppressing CLas from trifoliate orange plants eight months after treatment, and was harmful to citrus propagation, notably sweet orange. Antibiotic treatment did not eliminate CLas in sweet orange propagules, but a decrease in titer was recorded for both genotypes.
650 $aAmpicillin
650 $aCitrus
650 $aStreptomycin
700 1 $aSOUZA, U.
700 1 $aRAIOL‑JÚNIOR, L. L.
700 1 $aCARVALHO, E. V. de
700 1 $aMOREIRA, A. S.
700 1 $aASTUA, J. de F.
700 1 $aGIRARDI, E. A.
700 1 $aGESTEIRA, A. da S.
773 $tEuropean Journal of Plant Pathology, August 2024.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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| 3. |  | AFONSO, S. D. J.; LEDO, C. A. da S.; MOREIRA, R. F. C.; SILVA, S. de O. e; LEAL, V. D. de J.; CONCEIÇÃO, A. L. da S. Selection of descriptors in a morphological characteristics considered in cassava accessions by means of multivariate techniques. Journal of Agriculture and Veterinary Science, v. 7, Issue 1, p 13-20, Feb. 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 5 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 4. | | PEIXOTO, C. A. B.; REZENDE, J. de O.; SOUZA, L. da S.; PINTO, F. da C.; RIBEIRO, L. Q. P.; SANTOS, K. S.; SILVA, M. T.; LEAL, V. D. de J. Influência do sistema de plantio e do manejo do solo no desenvolvimento de cultivares de citros em Tabuleiros Costeiros. In: SEMINÁRIO DE PESQUISA DO RECÔNCAVO DA BAHIA, 1.; SEMINÁRIO ESTUDANTIL DE PESQUISA, 1.; SEMINÁRIO DE PÓS-GRADUAÇÃO DA UFRB, 1., 2007, Cruz das Almas. Ano internacional do planeta Terra. Cruz das Almas: UFRB, 2007.| Tipo: Resumo em Anais de Congresso |
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| Registros recuperados : 4 | |
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