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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
08/02/2021 |
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Data da última atualização: |
09/02/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
LEASTRO, M. O.; ASTUA, J. de F.; KITAJIMA, E. W.; PALLÁS, V.; NAVARRO, J. S. |
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Afiliação: |
MIKHAIL OLIVEIRA LEASTRO, Instituto Biológico, São Paulo; JULIANA DE FREITAS ASTUA, CNPMF; ELLIOT WATANABE KITAJIMA, ESALQ; VICENTE PALLÁS, Universidad Politécnica de Valencia; JESÚSA. SÁNCHEZ NAVARRO, Universidad Politécnica de Valencia. |
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Título: |
Unravelling the involvement of cilevirus p32 protein in the viral transport. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Scientifc Reports, n.11, v.2943, 2021. |
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Idioma: |
Inglês |
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Conteúdo: |
Citrus leprosis (CL) is a severe disease that affects citrus orchards mainly in Latin America. It is caused by Brevipalpus-transmitted viruses from genera Cilevirus and Dichorhavirus. Currently, no reports have explored the movement machinery for the cilevirus. Here, we have performed a detailed functional study of the p32 movement protein (MP) of two cileviruses. Citrus leprosis-associated viruses are not able to move systemically in neither their natural nor experimental host plants. However, here we show that cilevirus MPs are able to allow the cell-to-cell and long-distance transport of movement-defective alfalfa mosaic virus (AMV). Several features related with the viral transport were explored, including: (i) the ability of cilevirus MPs to facilitate virus movement on a nucleocapsid assembly independent-manner; (ii) the generation of tubular structures from transient expression in protoplast; (iii) the capability of the N- and C- terminus of MP to interact with the cognate capsid protein (p29) and; (iv) the role of the C-terminus of p32 in the cell-to-cell and long-distance transport, tubule formation and the MP-plasmodesmata co-localization. The MP was able to direct the p29 to the plasmodesmata, whereby the C-terminus of MP is independently responsible to recruit the p29 to the cell periphery. Furthermore, we report that MP possess the capacity to enter the nucleolus and to bind to a major nucleolar protein, the fibrillarin. Based on our findings, we provide a model for the role of the p32 in the intra- and intercellular viral spread. MenosCitrus leprosis (CL) is a severe disease that affects citrus orchards mainly in Latin America. It is caused by Brevipalpus-transmitted viruses from genera Cilevirus and Dichorhavirus. Currently, no reports have explored the movement machinery for the cilevirus. Here, we have performed a detailed functional study of the p32 movement protein (MP) of two cileviruses. Citrus leprosis-associated viruses are not able to move systemically in neither their natural nor experimental host plants. However, here we show that cilevirus MPs are able to allow the cell-to-cell and long-distance transport of movement-defective alfalfa mosaic virus (AMV). Several features related with the viral transport were explored, including: (i) the ability of cilevirus MPs to facilitate virus movement on a nucleocapsid assembly independent-manner; (ii) the generation of tubular structures from transient expression in protoplast; (iii) the capability of the N- and C- terminus of MP to interact with the cognate capsid protein (p29) and; (iv) the role of the C-terminus of p32 in the cell-to-cell and long-distance transport, tubule formation and the MP-plasmodesmata co-localization. The MP was able to direct the p29 to the plasmodesmata, whereby the C-terminus of MP is independently responsible to recruit the p29 to the cell periphery. Furthermore, we report that MP possess the capacity to enter the nucleolus and to bind to a major nucleolar protein, the fibrillarin. Based on our findings, we provide a model... Mostrar Tudo |
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Thesagro: |
Doença de Planta; Fruta Cítrica. |
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Thesaurus Nal: |
Citrus; Plant diseases and disorders. |
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Categoria do assunto: |
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Marc: |
LEADER 02172naa a2200217 a 4500
001 2129841
005 2021-02-09
008 2021 bl uuuu u00u1 u #d
100 1 $aLEASTRO, M. O.
245 $aUnravelling the involvement of cilevirus p32 protein in the viral transport.$h[electronic resource]
260 $c2021
520 $aCitrus leprosis (CL) is a severe disease that affects citrus orchards mainly in Latin America. It is caused by Brevipalpus-transmitted viruses from genera Cilevirus and Dichorhavirus. Currently, no reports have explored the movement machinery for the cilevirus. Here, we have performed a detailed functional study of the p32 movement protein (MP) of two cileviruses. Citrus leprosis-associated viruses are not able to move systemically in neither their natural nor experimental host plants. However, here we show that cilevirus MPs are able to allow the cell-to-cell and long-distance transport of movement-defective alfalfa mosaic virus (AMV). Several features related with the viral transport were explored, including: (i) the ability of cilevirus MPs to facilitate virus movement on a nucleocapsid assembly independent-manner; (ii) the generation of tubular structures from transient expression in protoplast; (iii) the capability of the N- and C- terminus of MP to interact with the cognate capsid protein (p29) and; (iv) the role of the C-terminus of p32 in the cell-to-cell and long-distance transport, tubule formation and the MP-plasmodesmata co-localization. The MP was able to direct the p29 to the plasmodesmata, whereby the C-terminus of MP is independently responsible to recruit the p29 to the cell periphery. Furthermore, we report that MP possess the capacity to enter the nucleolus and to bind to a major nucleolar protein, the fibrillarin. Based on our findings, we provide a model for the role of the p32 in the intra- and intercellular viral spread.
650 $aCitrus
650 $aPlant diseases and disorders
650 $aDoença de Planta
650 $aFruta Cítrica
700 1 $aASTUA, J. de F.
700 1 $aKITAJIMA, E. W.
700 1 $aPALLÁS, V.
700 1 $aNAVARRO, J. S.
773 $tScientifc Reports$gn.11
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Embrapa Mandioca e Fruticultura (CNPMF) |
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| 1. |  | MILORI, D. M. B. P.; PEREIRA, F. M. V.; VILLAS-BOAS, P. R.; CARDINALI, M. C. do B.; MARTIN-NETO, L.; FERREIRA, E. J.; MARTINS, P. K.; ASTUA, J. de F. Diagnóstico do HLB por técnicas fotônicas. In: ANDRADE, D. J.; FERREIRA, M. da C.; MARTINELLI, N. M. (Ed.). Aspectos da fitossanidade em citros. Jaboticabal: Cultura Acadêmica, 2014. p. 143-180.| Tipo: Capítulo em Livro Técnico-Científico |
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