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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
12/12/2019 |
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Data da última atualização: |
12/12/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GIGLIOTI, R.; BASSETO, C. C.; OKINO, C. H.; OLIVEIRA, H. N. de; OLIVEIRA, M. C. de S. |
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Afiliação: |
Rodrigo Giglioti, UNESP; César Cristiano Bassetto, UNESP; CINTIA HIROMI OKINO, CPPSE; Henrique Nunes de Oliveira, UNESP; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE. |
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Título: |
Development of a loop-mediated isothermal amplification (LAMP) assay for the detection of Anaplasma marginale. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Experimental and Applied Acarology, v. 77, n. 1, p.65?72, 2019. |
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Idioma: |
Inglês |
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Conteúdo: |
Parasitemia generated by Anaplasma marginale causes significant losses in the cattle industry. A major constraint to the effective control and management of anaplasmosis in livestock is the lack of a rapid and reliable diagnostic test to identify the parasite and allow for immediate therapy. In the present study, we developed a novel DNA-based assay for the detection of A. marginale in bovine blood samples, using loop-mediated isothermal amplification (LAMP). DNA from six cattle and hemoparasite samples (Babesia bovis, Babesia bigemina, Anaplasma centrale and A. marginale) were tested for specificity, sensitivity and cross-reactions. The developed LAMP procedures were also confirmed and compared with the qPCR method. The same gene sequence (major surface protein 1b, msp1b) of A. marginale was used to design a set of primers for the LAMP and qPCR assays. The results showed that LAMP is specific, as no positive signal was observed for the other tested hemoparasites. However, the sensitivity of the qPCR assay was ten times higher than LAMP. Our findings indicate that this LAMP method has a good sensitivity and high specificity for the detection of A. marginale and may have a potential application in the detection and differentiation of bovine anaplasmosis. |
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Palavras-Chave: |
Hemoparasita; Hemoparasite; Parasita; Parasite. |
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Thesagro: |
Babesia Bigemina; Babesia Bovis. |
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Thesaurus Nal: |
Animal parasites and pests; Bovine anaplasmosis; Parasites. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02101naa a2200277 a 4500
001 2116785
005 2019-12-12
008 2019 bl uuuu u00u1 u #d
100 1 $aGIGLIOTI, R.
245 $aDevelopment of a loop-mediated isothermal amplification (LAMP) assay for the detection of Anaplasma marginale.$h[electronic resource]
260 $c2019
520 $aParasitemia generated by Anaplasma marginale causes significant losses in the cattle industry. A major constraint to the effective control and management of anaplasmosis in livestock is the lack of a rapid and reliable diagnostic test to identify the parasite and allow for immediate therapy. In the present study, we developed a novel DNA-based assay for the detection of A. marginale in bovine blood samples, using loop-mediated isothermal amplification (LAMP). DNA from six cattle and hemoparasite samples (Babesia bovis, Babesia bigemina, Anaplasma centrale and A. marginale) were tested for specificity, sensitivity and cross-reactions. The developed LAMP procedures were also confirmed and compared with the qPCR method. The same gene sequence (major surface protein 1b, msp1b) of A. marginale was used to design a set of primers for the LAMP and qPCR assays. The results showed that LAMP is specific, as no positive signal was observed for the other tested hemoparasites. However, the sensitivity of the qPCR assay was ten times higher than LAMP. Our findings indicate that this LAMP method has a good sensitivity and high specificity for the detection of A. marginale and may have a potential application in the detection and differentiation of bovine anaplasmosis.
650 $aAnimal parasites and pests
650 $aBovine anaplasmosis
650 $aParasites
650 $aBabesia Bigemina
650 $aBabesia Bovis
653 $aHemoparasita
653 $aHemoparasite
653 $aParasita
653 $aParasite
700 1 $aBASSETO, C. C.
700 1 $aOKINO, C. H.
700 1 $aOLIVEIRA, H. N. de
700 1 $aOLIVEIRA, M. C. de S.
773 $tExperimental and Applied Acarology$gv. 77, n. 1, p.65?72, 2019.
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