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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos; Embrapa Gado de Leite. |
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Data corrente: |
18/03/2019 |
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Data da última atualização: |
10/01/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FONSECA, J. F. da; ZAMBRINI, F. N.; GUIMARÃES, J. D.; SILVA, M. R.; OLIVEIRA, M. E. F.; BARTLEWSKI, P. M.; SOUZA-FABJAN, J. M. G. |
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Afiliação: |
JEFERSON FERREIRA DA FONSECA, CNPC; UNIVERSIDADE FEDERAL DE VIÇOSA; UNIVERSIDADE FEDERAL DE VIÇOSA; MARCIO ROBERTO SILVA, CNPGL; UNIVERSIDADE ESTADUAL PAULISTA; UNIVERSITY OF GUELPH; UNIVERSIDADE FEDERAL FLUMINENSE. |
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Título: |
Cervical penetration rates and efficiency of non-surgical embryo recovery in estrous-synchronized Santa Inês ewes after administration of estradiol ester (benzoate or cypionate) in combination with d-cloprostenol and oxytocin. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Animal Reproduction Science, v. 203, p. 25-32, Apr. 2019. |
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DOI: |
10.1016/j.anireprosci.2019.02.004 |
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Idioma: |
Inglês |
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Conteúdo: |
The effects of estradiol esters, D-cloprostenol and oxytocin on induction of cervical dilation prior to non-surgical embryo recovery in Santa Inês ewes (Days 6–7 estrous cycle) were assessed in this study. In Trial 1, transcervical embryo flushing was performed in estrous-induced ewes administered 37.5 ug of D-cloprostenol i.m. 10 h before and 50 IU of oxytocin i.v. 20 min before uterine flushing with (EB-PGF-OT; n=13) or without (PGF-OT; n=11) 1 mg of estradiol benzoate i.m. administered concurrently with D-cloprostenol injection. In Trial 2, the estroussynchronized animals were treated with 1 mg of estradiol benzoate (EB-PGF-OT; n=12) or estradiol cypionate (EC-PGF-OT; n=12) i.m. along with 37.5 ug of D-cloprostenol i.m. 16 h before and 50 IU of oxytocin i.v. 20 min before uterine flushing. In Trial 1, uterine flushing could be accomplished in 38% of ewes in the EB-PGF-OT and 27% those in the PGF-OT (P>0.05) group. Flushing fluid recovery averaged 90% and there were 1.0 ± 1.1 embryos/ewe collected with mean duration of the flushing procedure being ˜36 min. In Trial 2, uterine flushing was accomplished in 78% of ewes in the EB-PGF-OT and 44% of those in the EC-PGF-OT group (P>0.05) with mean flushing fluid recovery rate being 88% and time elapsing to complete flushing being ˜33 min. Within the subsets of animals treated with EB, the percentages of successful transcervical penetrations were 38% compared with 78% in Trials 1 and 2, respectively (i.e., with EB administered 10 h compared with 16 h before uterine flushing: P<0.05). The interval from EB administration to the beginning of transcervical penetration can affect the efficacy of embryo recovery procedures utilizing a combined EB/d-cloprostenol/oxytocin pre-treatment. MenosThe effects of estradiol esters, D-cloprostenol and oxytocin on induction of cervical dilation prior to non-surgical embryo recovery in Santa Inês ewes (Days 6–7 estrous cycle) were assessed in this study. In Trial 1, transcervical embryo flushing was performed in estrous-induced ewes administered 37.5 ug of D-cloprostenol i.m. 10 h before and 50 IU of oxytocin i.v. 20 min before uterine flushing with (EB-PGF-OT; n=13) or without (PGF-OT; n=11) 1 mg of estradiol benzoate i.m. administered concurrently with D-cloprostenol injection. In Trial 2, the estroussynchronized animals were treated with 1 mg of estradiol benzoate (EB-PGF-OT; n=12) or estradiol cypionate (EC-PGF-OT; n=12) i.m. along with 37.5 ug of D-cloprostenol i.m. 16 h before and 50 IU of oxytocin i.v. 20 min before uterine flushing. In Trial 1, uterine flushing could be accomplished in 38% of ewes in the EB-PGF-OT and 27% those in the PGF-OT (P>0.05) group. Flushing fluid recovery averaged 90% and there were 1.0 ± 1.1 embryos/ewe collected with mean duration of the flushing procedure being ˜36 min. In Trial 2, uterine flushing was accomplished in 78% of ewes in the EB-PGF-OT and 44% of those in the EC-PGF-OT group (P>0.05) with mean flushing fluid recovery rate being 88% and time elapsing to complete flushing being ˜33 min. Within the subsets of animals treated with EB, the percentages of successful transcervical penetrations were 38% compared with 78% in Trials 1 and 2, respectively (i.e., with EB administered 10 ... Mostrar Tudo |
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Palavras-Chave: |
Cervical dilation; Estradiol esters; Transcervical embryo recovery. |
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Thesaurus Nal: |
Animal reproduction; Cloprostenol; Embryo transfer; Oxytocin; Sheep. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02814naa a2200301 a 4500
001 2107179
005 2025-01-10
008 2019 bl uuuu u00u1 u #d
024 7 $a10.1016/j.anireprosci.2019.02.004$2DOI
100 1 $aFONSECA, J. F. da
245 $aCervical penetration rates and efficiency of non-surgical embryo recovery in estrous-synchronized Santa Inês ewes after administration of estradiol ester (benzoate or cypionate) in combination with d-cloprostenol and oxytocin.$h[electronic resource]
260 $c2019
520 $aThe effects of estradiol esters, D-cloprostenol and oxytocin on induction of cervical dilation prior to non-surgical embryo recovery in Santa Inês ewes (Days 6–7 estrous cycle) were assessed in this study. In Trial 1, transcervical embryo flushing was performed in estrous-induced ewes administered 37.5 ug of D-cloprostenol i.m. 10 h before and 50 IU of oxytocin i.v. 20 min before uterine flushing with (EB-PGF-OT; n=13) or without (PGF-OT; n=11) 1 mg of estradiol benzoate i.m. administered concurrently with D-cloprostenol injection. In Trial 2, the estroussynchronized animals were treated with 1 mg of estradiol benzoate (EB-PGF-OT; n=12) or estradiol cypionate (EC-PGF-OT; n=12) i.m. along with 37.5 ug of D-cloprostenol i.m. 16 h before and 50 IU of oxytocin i.v. 20 min before uterine flushing. In Trial 1, uterine flushing could be accomplished in 38% of ewes in the EB-PGF-OT and 27% those in the PGF-OT (P>0.05) group. Flushing fluid recovery averaged 90% and there were 1.0 ± 1.1 embryos/ewe collected with mean duration of the flushing procedure being ˜36 min. In Trial 2, uterine flushing was accomplished in 78% of ewes in the EB-PGF-OT and 44% of those in the EC-PGF-OT group (P>0.05) with mean flushing fluid recovery rate being 88% and time elapsing to complete flushing being ˜33 min. Within the subsets of animals treated with EB, the percentages of successful transcervical penetrations were 38% compared with 78% in Trials 1 and 2, respectively (i.e., with EB administered 10 h compared with 16 h before uterine flushing: P<0.05). The interval from EB administration to the beginning of transcervical penetration can affect the efficacy of embryo recovery procedures utilizing a combined EB/d-cloprostenol/oxytocin pre-treatment.
650 $aAnimal reproduction
650 $aCloprostenol
650 $aEmbryo transfer
650 $aOxytocin
650 $aSheep
653 $aCervical dilation
653 $aEstradiol esters
653 $aTranscervical embryo recovery
700 1 $aZAMBRINI, F. N.
700 1 $aGUIMARÃES, J. D.
700 1 $aSILVA, M. R.
700 1 $aOLIVEIRA, M. E. F.
700 1 $aBARTLEWSKI, P. M.
700 1 $aSOUZA-FABJAN, J. M. G.
773 $tAnimal Reproduction Science$gv. 203, p. 25-32, Apr. 2019.
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Embrapa Caprinos e Ovinos (CNPC) |
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