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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
22/02/2016 |
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Data da última atualização: |
05/12/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
VIEIRA, D. D. S. S.; EMILIANIC, G.; MICHELOZZI, M.; CENTRITTO, M.; LUROE, F. S; MORILLON, R.; LORETO, F.; GESTEIRA, A. da S.; MASERTI, B. |
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Afiliação: |
DAYSE DRIELLY SOUZA SANTANA VIEIRA, CNR-IPSP; GIOVANNI EMILIANIC, CNR-IVALSA; MARCO MICHELOZZI, CNR-IBBR; MAURO CENTRITTO; FRANÇOIS LUROE; RAPHAËL MORILLON, UMR AGAP-INRA; FRANCESCO LORETO, NR-DISBA; ABELMON DA SILVA GESTEIRA, CNPMF; BIANCAELENA MASERTI, CNR-IPSP. |
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Título: |
Polyploidization alters constitutive content of volatile organic compounds (VOC) and improves membrane stability under water deficit in Volkamer lemon (Citrus limonia Osb.) leaves. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Environmental and Experimental Botany, n.126, p. 1-9, 2016. |
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ISSN: |
0098-8472 |
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Idioma: |
Inglês |
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Conteúdo: |
In Citrus species chromosome doubling naturally occurs in somatic embryos and doubled diploid plants often show better adaptation to adverse environmental condition. To understand the molecular determinants of stress acclimation, we examined the response to water deficit in diploid (2VL) and doubled diploid (4VL) seedlings of Volkamer lemon (Citrus limonia Osb.) assessing the profile of constitutive volatile organic compound (VOC) in control and stressed conditions. Physiological parameters and leaf volatile compound profiles were measured during water deficit and 24 h after rehydration of plants to field capacity. Net photosynthesis and stomatal conductance were reduced in water stressed leaves, with no significant differences between 2VL and 4VL plants. Malondialdehyde concentration, a marker of lipid peroxidation of cellular membranes, was significantly more higher in stressed 2VL leaves than in 4VL. The blend of constitutive VOC was different in control leaves being oxygenated sesquiterpenoids more abundant in 2VL leaves, and monoterpenoids more abundant in 4VL leaves. Water deficit did not stimulate biosynthesis of terpenoids, whereas accumulation of trans- 2 hexenal, a green leaf volatile (GLV) synthesized after membrane denaturation, was observed in stressed leaves of 2VL leaves, but not in 4VL leaves. Semiquantitative PCR showed an increase of the expression of HPL, the gene encoding for hydroperoxidase lyase which catalyzes 2-hexenal formation, only in 2VL plants. The expression of the putative dehydration transcription factor DREB2A was also observed only in 2VL water stressed plants. This work shows that level of ploidy may alter constitutive content of GLV by Citrus, therefore likely affecting plants capacity of protection and interaction with other organisms. Whereas diploid and double diploid plants showed similar physiological responses to water deficit, a biochemical marker indicated that membranes of double diploid leaves were more resistant to the stress.These results provide intriguing MenosIn Citrus species chromosome doubling naturally occurs in somatic embryos and doubled diploid plants often show better adaptation to adverse environmental condition. To understand the molecular determinants of stress acclimation, we examined the response to water deficit in diploid (2VL) and doubled diploid (4VL) seedlings of Volkamer lemon (Citrus limonia Osb.) assessing the profile of constitutive volatile organic compound (VOC) in control and stressed conditions. Physiological parameters and leaf volatile compound profiles were measured during water deficit and 24 h after rehydration of plants to field capacity. Net photosynthesis and stomatal conductance were reduced in water stressed leaves, with no significant differences between 2VL and 4VL plants. Malondialdehyde concentration, a marker of lipid peroxidation of cellular membranes, was significantly more higher in stressed 2VL leaves than in 4VL. The blend of constitutive VOC was different in control leaves being oxygenated sesquiterpenoids more abundant in 2VL leaves, and monoterpenoids more abundant in 4VL leaves. Water deficit did not stimulate biosynthesis of terpenoids, whereas accumulation of trans- 2 hexenal, a green leaf volatile (GLV) synthesized after membrane denaturation, was observed in stressed leaves of 2VL leaves, but not in 4VL leaves. Semiquantitative PCR showed an increase of the expression of HPL, the gene encoding for hydroperoxidase lyase which catalyzes 2-hexenal formation, only in 2VL plants. T... Mostrar Tudo |
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Thesagro: |
Fruta citríca. |
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Categoria do assunto: |
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Marc: |
LEADER 02826naa a2200241 a 4500
001 2037897
005 2016-12-05
008 2016 bl uuuu u00u1 u #d
022 $a0098-8472
100 1 $aVIEIRA, D. D. S. S.
245 $aPolyploidization alters constitutive content of volatile organic compounds (VOC) and improves membrane stability under water deficit in Volkamer lemon (Citrus limonia Osb.) leaves.$h[electronic resource]
260 $c2016
520 $aIn Citrus species chromosome doubling naturally occurs in somatic embryos and doubled diploid plants often show better adaptation to adverse environmental condition. To understand the molecular determinants of stress acclimation, we examined the response to water deficit in diploid (2VL) and doubled diploid (4VL) seedlings of Volkamer lemon (Citrus limonia Osb.) assessing the profile of constitutive volatile organic compound (VOC) in control and stressed conditions. Physiological parameters and leaf volatile compound profiles were measured during water deficit and 24 h after rehydration of plants to field capacity. Net photosynthesis and stomatal conductance were reduced in water stressed leaves, with no significant differences between 2VL and 4VL plants. Malondialdehyde concentration, a marker of lipid peroxidation of cellular membranes, was significantly more higher in stressed 2VL leaves than in 4VL. The blend of constitutive VOC was different in control leaves being oxygenated sesquiterpenoids more abundant in 2VL leaves, and monoterpenoids more abundant in 4VL leaves. Water deficit did not stimulate biosynthesis of terpenoids, whereas accumulation of trans- 2 hexenal, a green leaf volatile (GLV) synthesized after membrane denaturation, was observed in stressed leaves of 2VL leaves, but not in 4VL leaves. Semiquantitative PCR showed an increase of the expression of HPL, the gene encoding for hydroperoxidase lyase which catalyzes 2-hexenal formation, only in 2VL plants. The expression of the putative dehydration transcription factor DREB2A was also observed only in 2VL water stressed plants. This work shows that level of ploidy may alter constitutive content of GLV by Citrus, therefore likely affecting plants capacity of protection and interaction with other organisms. Whereas diploid and double diploid plants showed similar physiological responses to water deficit, a biochemical marker indicated that membranes of double diploid leaves were more resistant to the stress.These results provide intriguing
650 $aFruta citríca
700 1 $aEMILIANIC, G.
700 1 $aMICHELOZZI, M.
700 1 $aCENTRITTO, M.
700 1 $aLUROE, F. S
700 1 $aMORILLON, R.
700 1 $aLORETO, F.
700 1 $aGESTEIRA, A. da S.
700 1 $aMASERTI, B.
773 $tEnvironmental and Experimental Botany$gn.126, p. 1-9, 2016.
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| 1. |  | ARGOLO, L. R.; SOUZA, A. da S.; SOARES FILHO, W. dos S.; GESTEIRA, A. da S.; SANTOS, K. C. F. dos; DENISE DOS SANTOS VILA VERDE. Identificação de plantas triploides espontâneas em genótipos de citros. In: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 10., 2016: Cruz das Almas, BA. Traduzindo ciência para o mundo : resumos. Brasília, DF : Embrapa, 2016.| Tipo: Resumo em Anais de Congresso |
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