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Registro Completo |
Biblioteca(s): |
Embrapa Meio Ambiente. |
Data corrente: |
29/12/2015 |
Data da última atualização: |
25/01/2016 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
RODRIGUES, A. C. F.; MONERÓ, T. de O.; FRIGHETTO, R. T. S.; ALMEIDA, E. A. de. |
Afiliação: |
ALINE CRISTINA FERREIRA RODRIGUES, IBILCE-UNESP; TATIANA DE OLIVEIRA MONERÓ, IBILCE-UNESP; ROSA TOYOKO SHIRAISHI FRIGHETTO, CNPMA; EDUARDO ALVES DE ALMEIDA, IBILCE-UNESP. |
Título: |
E2 potentializes benzo(a)pyrene-induced hepatic cytochrome P450 enzyme activities in Nile tilapia at high concentrations. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
Environmental Science & Pollution Research, Berlin, v. 22, n. 22, p. 17367-17374, 2015. |
Idioma: |
Inglês |
Conteúdo: |
In the aquatic environment, biotransformation enzymes are established biomarkers for assessing PAH exposure in fish, but little is known about the effect of 17b-estradiol (E2) on these enzymes during exposure to benzo(a)pyrene (BaP). In this study, Nile tilapia ( Oreochromis niloticus) were exposed for 3, 5, and 10 days to BaP (300 μg L) and E2 (5 μg L). These substances were applied isolated or mixed. In the mixture experiment, fish were analyzed pre- and postexposure in order to better understand whether preexposure to the hormone masks the responses activated by PAH or vice versa. Phase I enzymes ethoxyresorufin-O-deethylase (EROD), pentoxyresorufin-O-depenthylase (PROD), and benzyloxyresorufin-O-debenzylase (BROD) activities as well as the phase II enzyme glutathione S-transferase (GST) were analyzed. Isolated E2 treatment decreased EROD activity after 3 days, but this enzyme activity returned to control values after 5 and 10 days of exposure. Isolated BaP treatment significantly induced EROD activity after 3 and 5 days, and the activity returned to control levels after ten exposure days. Combined treatment (E2 + Bap) significantly increased EROD activity, both in the pre- and postexposure. This increase was even higher than in the isolated BaP treatment, suggesting a synergism between these two compounds. When E2 and BaP were used singly, they did not change BROD and PROD activities. However, combined treatment (E2 + Bap) significantly increased PROD activity. Isolated BaP treatment increased GST activity after 10 days. However, this response was not observed in the mixture treatment, suggesting that E2 suppressed the GST induction modulated by BaP. The results put together indicated that E2 altered the biotransformation pathway regarding enzymes activated by BaP in Nile tilapia. MenosIn the aquatic environment, biotransformation enzymes are established biomarkers for assessing PAH exposure in fish, but little is known about the effect of 17b-estradiol (E2) on these enzymes during exposure to benzo(a)pyrene (BaP). In this study, Nile tilapia ( Oreochromis niloticus) were exposed for 3, 5, and 10 days to BaP (300 μg L) and E2 (5 μg L). These substances were applied isolated or mixed. In the mixture experiment, fish were analyzed pre- and postexposure in order to better understand whether preexposure to the hormone masks the responses activated by PAH or vice versa. Phase I enzymes ethoxyresorufin-O-deethylase (EROD), pentoxyresorufin-O-depenthylase (PROD), and benzyloxyresorufin-O-debenzylase (BROD) activities as well as the phase II enzyme glutathione S-transferase (GST) were analyzed. Isolated E2 treatment decreased EROD activity after 3 days, but this enzyme activity returned to control values after 5 and 10 days of exposure. Isolated BaP treatment significantly induced EROD activity after 3 and 5 days, and the activity returned to control levels after ten exposure days. Combined treatment (E2 + Bap) significantly increased EROD activity, both in the pre- and postexposure. This increase was even higher than in the isolated BaP treatment, suggesting a synergism between these two compounds. When E2 and BaP were used singly, they did not change BROD and PROD activities. However, combined treatment (E2 + Bap) significantly increased PROD activity. Isolate... Mostrar Tudo |
Palavras-Chave: |
17b-Estradiol; Biotransformation enzymes; Hidrocarboneto aromático policíclico. |
Thesagro: |
Oreochromis niloticus; Poluição da água; Substância tóxica; Tilápia nilótica. |
Thesaurus Nal: |
Benzopyrenes; Polycyclic aromatic hydrocarbons; Water pollution. |
Categoria do assunto: |
H Saúde e Patologia |
Marc: |
LEADER 02768naa a2200277 a 4500 001 2032529 005 2016-01-25 008 2015 bl uuuu u00u1 u #d 100 1 $aRODRIGUES, A. C. F. 245 $aE2 potentializes benzo(a)pyrene-induced hepatic cytochrome P450 enzyme activities in Nile tilapia at high concentrations.$h[electronic resource] 260 $c2015 520 $aIn the aquatic environment, biotransformation enzymes are established biomarkers for assessing PAH exposure in fish, but little is known about the effect of 17b-estradiol (E2) on these enzymes during exposure to benzo(a)pyrene (BaP). In this study, Nile tilapia ( Oreochromis niloticus) were exposed for 3, 5, and 10 days to BaP (300 μg L) and E2 (5 μg L). These substances were applied isolated or mixed. In the mixture experiment, fish were analyzed pre- and postexposure in order to better understand whether preexposure to the hormone masks the responses activated by PAH or vice versa. Phase I enzymes ethoxyresorufin-O-deethylase (EROD), pentoxyresorufin-O-depenthylase (PROD), and benzyloxyresorufin-O-debenzylase (BROD) activities as well as the phase II enzyme glutathione S-transferase (GST) were analyzed. Isolated E2 treatment decreased EROD activity after 3 days, but this enzyme activity returned to control values after 5 and 10 days of exposure. Isolated BaP treatment significantly induced EROD activity after 3 and 5 days, and the activity returned to control levels after ten exposure days. Combined treatment (E2 + Bap) significantly increased EROD activity, both in the pre- and postexposure. This increase was even higher than in the isolated BaP treatment, suggesting a synergism between these two compounds. When E2 and BaP were used singly, they did not change BROD and PROD activities. However, combined treatment (E2 + Bap) significantly increased PROD activity. Isolated BaP treatment increased GST activity after 10 days. However, this response was not observed in the mixture treatment, suggesting that E2 suppressed the GST induction modulated by BaP. The results put together indicated that E2 altered the biotransformation pathway regarding enzymes activated by BaP in Nile tilapia. 650 $aBenzopyrenes 650 $aPolycyclic aromatic hydrocarbons 650 $aWater pollution 650 $aOreochromis niloticus 650 $aPoluição da água 650 $aSubstância tóxica 650 $aTilápia nilótica 653 $a17b-Estradiol 653 $aBiotransformation enzymes 653 $aHidrocarboneto aromático policíclico 700 1 $aMONERÓ, T. de O. 700 1 $aFRIGHETTO, R. T. S. 700 1 $aALMEIDA, E. A. de 773 $tEnvironmental Science & Pollution Research, Berlin$gv. 22, n. 22, p. 17367-17374, 2015.
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Embrapa Meio Ambiente (CNPMA) |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
Data corrente: |
29/11/1997 |
Data da última atualização: |
04/11/2015 |
Autoria: |
TOZZATO, E.; BONATO, P. S.; CERDEIRA, A. L.; GOMES, M. A. F.; CARVALHO, D.; LANCHOTE, V. L.; QUEIROZ, R. H. C. |
Afiliação: |
USP; USP; ANTONIO LUIZ CERDEIRA, CNPMA; MARCO ANTONIO FERREIRA GOMES, CNPMA; USP; USP; USP. |
Título: |
Method for bioassaying ametryne in water. |
Ano de publicação: |
1997 |
Fonte/Imprenta: |
In: CONGRESS OF PHARMACEUTICAL SCIENCES, 1., 1997,Ribeirao Preto, SP. Bollettino Chimico Farmaceutico, v.136, p.49, 1997. Abstract. |
Idioma: |
Inglês |
Conteúdo: |
The beneficial effects of herbicides are sometimes mitigated by their persistence in the environment. Water may be contaminated with herbicides from aerial spraying, runoff from land treated with herbicides, and direct introduction of herbicides into water to control aquatic weeds. The availability of a monitoring system for herbicides, particularly in flowing wates such as irrigation canals is essential so that damage to the environment and crop plants through the use of contaminated water can ve avoided. Therefore, a simple and rapid quantitative method is presented for the screening and determination of small amounts of ametryne in aqueous solution. This method is based on the activity of the ametryne in inhibition the growth of the primary root and shoot of germinating Lactuca sativa seed. Solutions of ametryne of unknown concentration, may be subjected to the procedure herein described, and growth inhibition may be expressed in percentage of control. This may then be compared with the standard curve, and the presumptive concentration of ametryne may be thereby ascertained. It is sensitive to 0.02 ug/l and is applicable from this concentration to 2 ug/l. Initial surface sterilization of the seed, selection of pregerminated seed of certain root lengths, and special equipment are not necessary. |
Palavras-Chave: |
Ametryne; Método de bioensaio. |
Categoria do assunto: |
W Química e Física |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/87149/1/1997RAs-007.PDF
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Marc: |
LEADER 01970nam a2200205 a 4500 001 1012761 005 2015-11-04 008 1997 bl uuuu u00u1 u #d 100 1 $aTOZZATO, E. 245 $aMethod for bioassaying ametryne in water.$h[electronic resource] 260 $aIn: CONGRESS OF PHARMACEUTICAL SCIENCES, 1., 1997,Ribeirao Preto, SP. Bollettino Chimico Farmaceutico, v.136, p.49, 1997. Abstract.$c1997 520 $aThe beneficial effects of herbicides are sometimes mitigated by their persistence in the environment. Water may be contaminated with herbicides from aerial spraying, runoff from land treated with herbicides, and direct introduction of herbicides into water to control aquatic weeds. The availability of a monitoring system for herbicides, particularly in flowing wates such as irrigation canals is essential so that damage to the environment and crop plants through the use of contaminated water can ve avoided. Therefore, a simple and rapid quantitative method is presented for the screening and determination of small amounts of ametryne in aqueous solution. This method is based on the activity of the ametryne in inhibition the growth of the primary root and shoot of germinating Lactuca sativa seed. Solutions of ametryne of unknown concentration, may be subjected to the procedure herein described, and growth inhibition may be expressed in percentage of control. This may then be compared with the standard curve, and the presumptive concentration of ametryne may be thereby ascertained. It is sensitive to 0.02 ug/l and is applicable from this concentration to 2 ug/l. Initial surface sterilization of the seed, selection of pregerminated seed of certain root lengths, and special equipment are not necessary. 653 $aAmetryne 653 $aMétodo de bioensaio 700 1 $aBONATO, P. S. 700 1 $aCERDEIRA, A. L. 700 1 $aGOMES, M. A. F. 700 1 $aCARVALHO, D. 700 1 $aLANCHOTE, V. L. 700 1 $aQUEIROZ, R. H. C.
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