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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
29/07/2015 |
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Data da última atualização: |
18/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
RAMOS-GONZALEZ, P. L.; SARUBBI-ORUE, H.; GONZALES-SEGNANA, L.; CHABI-JESUS, C.; ASTUA, J. de F.; KITAJIMA, E. W. |
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Afiliação: |
PEDRO LUIS RAMOS-GONZALEZ, IAC; HUMBERTO SARUBBI-ORUE, Universidad Nacional de Asuncion; LUIS GONZALES-SEGNANA, Universidad Nacional de Asuncion; CAMILA CHABI-JESUS, IAC; JULIANA DE FREITAS ASTUA, CNPMF; ELIOT WATANABE KITAJIMA, ESALQ. |
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Título: |
Orchid Fleck Virus Infecting Orchids in Paraguay: First Report and Use of Degenerate Primers for its Detection. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Journal of Phytopathology, 2015. |
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Páginas: |
6p. |
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Série: |
1439-0434 |
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Idioma: |
Inglês |
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Conteúdo: |
The presence of Orchid fleck virus (OFV) in Paraguay was confirmed in orchid plants collected during a survey carried out in 2013. Leaves displayed ringspot and fleck symptoms, and in infected tissues, non-enveloped, short, rodlike viral particles were observed. Partial OFV N and L genes were amplified using specific and degenerate primers, respectively; the nucleotide sequences demonstrated high identities (98%) with other OFV isolates. Degenerate primers for the L gene were designed considering conserved regions within all of the available OFV sequences and those from the closely related isolates citrus leprosis virus nuclear type (CiLV-N) and citrus necrotic spot virus. Degenerate primers were also successfully used for the detection of CiLV-N from infected citrus samples. |
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Palavras-Chave: |
Citrus leprosis virus. |
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Thesagro: |
Doença de planta; Orchidaceae; Orquídea. |
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Categoria do assunto: |
O Insetos e Entomologia |
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Marc: |
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008 2015 bl uuuu u00u1 u #d
100 1 $aRAMOS-GONZALEZ, P. L.
245 $aOrchid Fleck Virus Infecting Orchids in Paraguay$bFirst Report and Use of Degenerate Primers for its Detection.$h[electronic resource]
260 $c2015
300 $a6p.
490 $a1439-0434
520 $aThe presence of Orchid fleck virus (OFV) in Paraguay was confirmed in orchid plants collected during a survey carried out in 2013. Leaves displayed ringspot and fleck symptoms, and in infected tissues, non-enveloped, short, rodlike viral particles were observed. Partial OFV N and L genes were amplified using specific and degenerate primers, respectively; the nucleotide sequences demonstrated high identities (98%) with other OFV isolates. Degenerate primers for the L gene were designed considering conserved regions within all of the available OFV sequences and those from the closely related isolates citrus leprosis virus nuclear type (CiLV-N) and citrus necrotic spot virus. Degenerate primers were also successfully used for the detection of CiLV-N from infected citrus samples.
650 $aDoença de planta
650 $aOrchidaceae
650 $aOrquídea
653 $aCitrus leprosis virus
700 1 $aSARUBBI-ORUE, H.
700 1 $aGONZALES-SEGNANA, L.
700 1 $aCHABI-JESUS, C.
700 1 $aASTUA, J. de F.
700 1 $aKITAJIMA, E. W.
773 $tJournal of Phytopathology, 2015.
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| 1. |  | RODRIGUEZ, M. A. D.; WAALWIJK, C.; DIAZ, C.; D'HONT, A.; YAHIAOUE, N.; BAKRLY, F.; SOUZA JR. M.; KEMA, G. H. J. Characterization of Musa germplasm for resistance to tropical race 4 of fusarium oxysporum f. sp. cubense. In: PROMUSA SYMPOSIUM, 2011, Salvador. Bananas and plantains: toward sustainable global production and improved uses: abstracts. [Montepellier.]: [Bioversity International, 2011. p.37.| Tipo: Resumo em Anais de Congresso |
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