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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
28/07/2015 |
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Data da última atualização: |
03/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CARMO, C. D.; SANTOS, D. B.; ALVES, L. B.; OLIVEIRA, G. A. F.; OLIVEIRA, E. J. de. |
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Afiliação: |
C. D. CARMO, UFRB; D. B. SANTOS, UFRB; L. B. ALVES, UFRB; G. A. F. OLIVEIRA, UFRB; EDER JORGE DE OLIVEIRA, CNPMF. |
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Título: |
Development of TRAP (Target Region Amplification Polymorphism) as New Tool for Molecular Genetic Analysis in Cassava. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Plant Molecular Biology Reporte, 18 April 2015. |
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Páginas: |
14P. |
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Idioma: |
Inglês |
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Conteúdo: |
Cassava (Manihot esculenta Crantz) lacks molecular studies for use in breeding and germplasm bank maintenance. This work aimed to develop and validate TRAP (target region amplification olymorphism) markers for cassava and evaluate their potential for structuring the genetic diversity of this species. Preliminary analyses with 396 combinations found 64%of combinations with a good amplification pattern and polymorphism. The 69 most polymorphic TRAP combinations were used to characterize 46 cassava genotypes, from which 606 alleles (range 3 to 18 with a mean of 8.8 alleles per combination) were identified. The polymorphic information content (PIC) ranged from 0.03 to 0.38 (average 0.23), while 31 combinations showed a PIC >0.25. The resolving power (Rp) parameter ranged from 0.10 to 6.30 (average 3.21). The primers that were related to starch and carotenoid biosynthesis, cyanogenic compounds, post-harvest physiological deterioration, root formation, and defense responses were the most polymorphic (>70 % of polymorphic fragments, PIC>0.25, and Rp>3.21). A total of 37 private alleles were identified in 20 accessions. Bayesian clustering as implemented in STRU CTURE revealed the presence of two major clusters (K=2) and four subclusters (K=4). The group differentiation based on the molecular variance analysis (AMOVA) showed that most of the genetic variation is within groups but with significant differences between groups. Therefore, TRAP primers have a high polymorphism for use as a molecular tool in cassava, in addition to the association with genetic regions that may increase the chances of obtaining functional markers. MenosCassava (Manihot esculenta Crantz) lacks molecular studies for use in breeding and germplasm bank maintenance. This work aimed to develop and validate TRAP (target region amplification olymorphism) markers for cassava and evaluate their potential for structuring the genetic diversity of this species. Preliminary analyses with 396 combinations found 64%of combinations with a good amplification pattern and polymorphism. The 69 most polymorphic TRAP combinations were used to characterize 46 cassava genotypes, from which 606 alleles (range 3 to 18 with a mean of 8.8 alleles per combination) were identified. The polymorphic information content (PIC) ranged from 0.03 to 0.38 (average 0.23), while 31 combinations showed a PIC >0.25. The resolving power (Rp) parameter ranged from 0.10 to 6.30 (average 3.21). The primers that were related to starch and carotenoid biosynthesis, cyanogenic compounds, post-harvest physiological deterioration, root formation, and defense responses were the most polymorphic (>70 % of polymorphic fragments, PIC>0.25, and Rp>3.21). A total of 37 private alleles were identified in 20 accessions. Bayesian clustering as implemented in STRU CTURE revealed the presence of two major clusters (K=2) and four subclusters (K=4). The group differentiation based on the molecular variance analysis (AMOVA) showed that most of the genetic variation is within groups but with significant differences between groups. Therefore, TRAP primers have a high polymorphism for use as... Mostrar Tudo |
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Palavras-Chave: |
Marcador molelular. |
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Thesagro: |
Mandioca; Marcador genético. |
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Thesaurus Nal: |
Cassava; Genetic markers. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02362naa a2200241 a 4500
001 2020697
005 2023-03-03
008 2015 bl uuuu u00u1 u #d
100 1 $aCARMO, C. D.
245 $aDevelopment of TRAP (Target Region Amplification Polymorphism) as New Tool for Molecular Genetic Analysis in Cassava.$h[electronic resource]
260 $c2015
300 $a14P.
520 $aCassava (Manihot esculenta Crantz) lacks molecular studies for use in breeding and germplasm bank maintenance. This work aimed to develop and validate TRAP (target region amplification olymorphism) markers for cassava and evaluate their potential for structuring the genetic diversity of this species. Preliminary analyses with 396 combinations found 64%of combinations with a good amplification pattern and polymorphism. The 69 most polymorphic TRAP combinations were used to characterize 46 cassava genotypes, from which 606 alleles (range 3 to 18 with a mean of 8.8 alleles per combination) were identified. The polymorphic information content (PIC) ranged from 0.03 to 0.38 (average 0.23), while 31 combinations showed a PIC >0.25. The resolving power (Rp) parameter ranged from 0.10 to 6.30 (average 3.21). The primers that were related to starch and carotenoid biosynthesis, cyanogenic compounds, post-harvest physiological deterioration, root formation, and defense responses were the most polymorphic (>70 % of polymorphic fragments, PIC>0.25, and Rp>3.21). A total of 37 private alleles were identified in 20 accessions. Bayesian clustering as implemented in STRU CTURE revealed the presence of two major clusters (K=2) and four subclusters (K=4). The group differentiation based on the molecular variance analysis (AMOVA) showed that most of the genetic variation is within groups but with significant differences between groups. Therefore, TRAP primers have a high polymorphism for use as a molecular tool in cassava, in addition to the association with genetic regions that may increase the chances of obtaining functional markers.
650 $aCassava
650 $aGenetic markers
650 $aMandioca
650 $aMarcador genético
653 $aMarcador molelular
700 1 $aSANTOS, D. B.
700 1 $aALVES, L. B.
700 1 $aOLIVEIRA, G. A. F.
700 1 $aOLIVEIRA, E. J. de
773 $tPlant Molecular Biology Reporte, 18 April 2015.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| 1. |  | RODRIGUEZ, M. A. D.; WAALWIJK, C.; MUTUA, P.; DALY, A.; CHANG, P. F. L.; CORCOLON, B. M.; KEMA, G. H. J. Detecting fusarium oxysporum f. Sp. cubense tropical race 4 in soil and symptomless banana tissues. In:PROMUSA SYMPOSIUM, 2011, Salvador. Bananas and plantains: toward sustainable global production and improved uses: abstracts. [Montepellier.]: [Bioversity International, 2011. p. 36.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 2. | | RODRIGUEZ, M. A. D.; WAALWIJK, C.; MUTUA, P.; DALY, A.; CHANG, P. F. L.; CORCOLON, B. M.; PAIVA, L.; SOUZA, M.; KEMA, G. H. J. Detecting Fusarium oxysporum f. sp. cubense tropical race 4 in soil and symptomless banana tissues. Acta Horticulturae, n. 986, p. 127-130, April, 2013. Trabalho apresentado no ISHS - ProMusa Symposium on Bananas and Plantains: Towards Sustainable Global Production and Improved Uses. Eds.: I. Van den Bergh et al.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| Registros recuperados : 2 | |
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