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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
21/05/2015 |
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Data da última atualização: |
18/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOARES, T. L.; SOUZA, E. H. de; SAMPAIO, L. F. S.; COSTA, M. A. P. de C.; SILVA, S. de O. e; SEREJO, J. A. dos S. |
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Afiliação: |
TALIANE LEILA SOARES; EVERTON HILO DE SOUZA; LAECIO FERNANDES SOUZA SAMPAIO; MARIA ANGÉLICA PEREIRA DE CARVALHO COSTA; SEBASTIÃO DE OLIVEIRA E SILVA; JANAY ALMEIDA DOS SANTOS SEREJO, CNPMF. |
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Título: |
Effect of collection time on the viability of banana pollen grains. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
African Journal of Biotechnology, v.14, n. 4, p.1207-1214, April, 2015. |
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ISSN: |
1684-5315 |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of this study was to assess the pollen viability of six improve ddiploid banana plants (AA) collected in different periods of the day , in two seasons of the year (winter and summer), usingin vitro germination test and staining. Pollen grains collected at 8 am (anthesis), 10am, 12pm, 2pm and 4pm were evaluated . We used a culture medium for pollen germination containing 15% sucrose, 0.01% H3BO3, 0.01%KNO3, 0.03%Ca(NO3)2.4H2O, and 0.02%MgSO4.7H2O, solidified with 0.8% agar, adjusted todifferent pH. The pollen viability was evaluated by staining with 2,3,5-triphenyltetrazolium chloride(TTC).The highest pollen germination rates and viability were obtained at 8amand the lowest at 4pm, in both seasons. The average in vitro germination percentage and viability level were negatively influenced by the number of hours after anthesis. During the summer,the pollen viability and in vitrogermination rates werehighest when compared to winter period. The results presented can help at the selection of genetic materials and enable inferences on the best pollencollection time for use in cross breedingprograms for plant improvemen. |
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Thesagro: |
Banana. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01753naa a2200205 a 4500
001 2015985
005 2023-05-18
008 2015 bl uuuu u00u1 u #d
022 $a1684-5315
100 1 $aSOARES, T. L.
245 $aEffect of collection time on the viability of banana pollen grains.$h[electronic resource]
260 $c2015
520 $aThe objective of this study was to assess the pollen viability of six improve ddiploid banana plants (AA) collected in different periods of the day , in two seasons of the year (winter and summer), usingin vitro germination test and staining. Pollen grains collected at 8 am (anthesis), 10am, 12pm, 2pm and 4pm were evaluated . We used a culture medium for pollen germination containing 15% sucrose, 0.01% H3BO3, 0.01%KNO3, 0.03%Ca(NO3)2.4H2O, and 0.02%MgSO4.7H2O, solidified with 0.8% agar, adjusted todifferent pH. The pollen viability was evaluated by staining with 2,3,5-triphenyltetrazolium chloride(TTC).The highest pollen germination rates and viability were obtained at 8amand the lowest at 4pm, in both seasons. The average in vitro germination percentage and viability level were negatively influenced by the number of hours after anthesis. During the summer,the pollen viability and in vitrogermination rates werehighest when compared to winter period. The results presented can help at the selection of genetic materials and enable inferences on the best pollencollection time for use in cross breedingprograms for plant improvemen.
650 $aBanana
700 1 $aSOUZA, E. H. de
700 1 $aSAMPAIO, L. F. S.
700 1 $aCOSTA, M. A. P. de C.
700 1 $aSILVA, S. de O. e
700 1 $aSEREJO, J. A. dos S.
773 $tAfrican Journal of Biotechnology$gv.14, n. 4, p.1207-1214, April, 2015.
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