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Registro Completo |
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
24/12/2016 |
Data da última atualização: |
24/12/2016 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SOARES, T. L.; SOUZA, E. H. de; COSTA, M. A. P. de C.; SILVA, S. de O. e; SEREJO, J. A. dos S. |
Afiliação: |
TALIANE LEILA SOARES; EVERTON HILO DE SOUZA; MARIA ANGÉLICA PEREIRA DE CARVALHO COSTA, UFRB; SEBASTIÃO DE OLIVEIRA E SILVA, UFRB; JANAY ALMEIDA DOS SANTOS SEREJO, CNPMF. |
Título: |
Viability of pollen grains of tetraploid banana. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Bragantia, Campinas, v. 75, n. 2, p.145-151, 2016. |
ISSN: |
1678-4499 |
Idioma: |
Inglês |
Conteúdo: |
Obtaining banana tetraploid cultivars from triploid strains results in total or partial reestablishment of fertility, allowing the occurrence of some fruits with seeds, a feature that is undesirable from a marketing perspective. The objective of this study was to assess the viability of pollen of 12 banana tetraploid hybrids (AAAB) by means of in vitro germination and two histochemical tests (acetocarmine and 2,3,5-triphenyltetrazolium chloride). The pollen tube growth was evaluated by germinating grains in three culture media ? M1: 0.03% Ca(NO3)?4H2O, 0.02% Mg(SO4)?7H2O, 0.01% KNO3, 0.01% H3BO3 and 15% sucrose; M2: 0.03% Ca(NO3)?4H2O, 0.01% KNO3, 0.01% H3BO3 and 10% sucrose; and M3: 0.015% H3BO3, 0.045% Ca3(PO4)2 and 25% sucrose. The acetocarmine staining indicated high viability (above 80%), except for the genotypes YB42-17 and Caprichosa, which were 76 and 70%, respectively. However, the in vitro germination rate was lower than 50% for all the genotypes, except for the hybrids YB42-17 (M1) and YB42-47 (M1). The medium M1 provided the greatest germination percentage and pollen tube growth. Among the genotypes assessed, YB42-47 presented the highest germination rate (61.5%) and tube length (5.0 mm). On the other hand, the Vitória cultivar had the lowest germination percentage (8.2%) in medium M1. Studies of meiosis can shed more light on the differences observed in the evaluated tetraploids, since meiotic irregularities can affect pollen viability. |
Thesagro: |
Banana. |
Categoria do assunto: |
G Melhoramento Genético |
Marc: |
LEADER 02009naa a2200193 a 4500 001 2059262 005 2016-12-24 008 2016 bl uuuu u00u1 u #d 022 $a1678-4499 100 1 $aSOARES, T. L. 245 $aViability of pollen grains of tetraploid banana.$h[electronic resource] 260 $c2016 520 $aObtaining banana tetraploid cultivars from triploid strains results in total or partial reestablishment of fertility, allowing the occurrence of some fruits with seeds, a feature that is undesirable from a marketing perspective. The objective of this study was to assess the viability of pollen of 12 banana tetraploid hybrids (AAAB) by means of in vitro germination and two histochemical tests (acetocarmine and 2,3,5-triphenyltetrazolium chloride). The pollen tube growth was evaluated by germinating grains in three culture media ? M1: 0.03% Ca(NO3)?4H2O, 0.02% Mg(SO4)?7H2O, 0.01% KNO3, 0.01% H3BO3 and 15% sucrose; M2: 0.03% Ca(NO3)?4H2O, 0.01% KNO3, 0.01% H3BO3 and 10% sucrose; and M3: 0.015% H3BO3, 0.045% Ca3(PO4)2 and 25% sucrose. The acetocarmine staining indicated high viability (above 80%), except for the genotypes YB42-17 and Caprichosa, which were 76 and 70%, respectively. However, the in vitro germination rate was lower than 50% for all the genotypes, except for the hybrids YB42-17 (M1) and YB42-47 (M1). The medium M1 provided the greatest germination percentage and pollen tube growth. Among the genotypes assessed, YB42-47 presented the highest germination rate (61.5%) and tube length (5.0 mm). On the other hand, the Vitória cultivar had the lowest germination percentage (8.2%) in medium M1. Studies of meiosis can shed more light on the differences observed in the evaluated tetraploids, since meiotic irregularities can affect pollen viability. 650 $aBanana 700 1 $aSOUZA, E. H. de 700 1 $aCOSTA, M. A. P. de C. 700 1 $aSILVA, S. de O. e 700 1 $aSEREJO, J. A. dos S. 773 $tBragantia, Campinas$gv. 75, n. 2, p.145-151, 2016.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
22/11/2020 |
Data da última atualização: |
23/11/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
MELO, B. P. de; LOURENCO-TESSUTTI, I. T.; PAIXÃO, J. F. R.; NORIEGA, D. D.; SILVA, M. C. M.; ALMEIDA-ENGLER, J. de; FONTES, E. P. B.; GROSSI-DE-SA, M. F. |
Afiliação: |
BRUNO PAES DE MELO, UFV; ISABELA TRISTAN LOURENCO TESSUTTI, Cenargen; JOAQUIN FELIPE ROCA PAIXÃO, UFRJ; DANIEL DAVID NORIEGA, UCB; MARIA CRISTINA MATTAR DA SILVA, Cenargen; JANICE DE ALMEIDA-ENGLER, UMR INSTITUT SOPHIA AGROBITECH INRA/CNRS/UNS, FRANCE; ELIZABETH PACHECO BATISTA FONTES, UFV; MARIA FATIMA GROSSI DE SA, Cenargen. |
Título: |
Transcriptional modulation of AREB-1 by cRiSpRa improves plant physiological performance under severe water deficit. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Scientific Reports, v. 10, 16231, 2020. |
DOI: |
https://doi.org/10.1038/s41598-020-72464-y |
Idioma: |
Inglês |
Palavras-Chave: |
AREB-1; CRISPRa; Water deficit. |
Thesaurus NAL: |
Drought tolerance; Genetic engineering. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/218071/1/s41598-020-72464-y.pdf
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Marc: |
LEADER 00842naa a2200265 a 4500 001 2126905 005 2020-11-23 008 2020 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1038/s41598-020-72464-y$2DOI 100 1 $aMELO, B. P. de 245 $aTranscriptional modulation of AREB-1 by cRiSpRa improves plant physiological performance under severe water deficit.$h[electronic resource] 260 $c2020 650 $aDrought tolerance 650 $aGenetic engineering 653 $aAREB-1 653 $aCRISPRa 653 $aWater deficit 700 1 $aLOURENCO-TESSUTTI, I. T. 700 1 $aPAIXÃO, J. F. R. 700 1 $aNORIEGA, D. D. 700 1 $aSILVA, M. C. M. 700 1 $aALMEIDA-ENGLER, J. de 700 1 $aFONTES, E. P. B. 700 1 $aGROSSI-DE-SA, M. F. 773 $tScientific Reports$gv. 10, 16231, 2020.
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