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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
04/12/2017 |
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Data da última atualização: |
04/12/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, R. L. da; SOUZA, E. H. de; VIEIRA, L. de J.; PELACANI, C. R.; SOUZA, F. V. D. |
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Afiliação: |
RONILZE LEITE DA SILVA, EFS; EVERTON HILO DE SOUZA, CAPES; LÍVIA DE JESUS VIEIRA, CAPES; CLAUDINÉIA REGINA PELACANI, UEFS; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF. |
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Título: |
Cryopreservation of pollen of wild pineapple accessions. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Scientia Horticulturae, v.219, p.326-334, May 2017. |
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Idioma: |
Inglês |
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Conteúdo: |
Cryopreservation of pollen is an additional way to conserve many species. In particular, it can be used as an alternative strategy to preserve the genetic variation of the genus Ananas. The aim of this study was to cryopreserve pollen from wild pineapple accessions to conserve a gene pool of the genus Ananas, as well as to minimize problems of asynchronous flowering among accessions preserved in a germplasm bank. Accessions of all botanical varieties were used to investigate different protocols for freezing pollen, as well as to determine their viability in vitro/in vivo during the investigation and after freezing in liquid nitrogen. Anthers with pollen of the different accessions were dehydrated and then immersed in liquid nitrogen for periods of 24 h, 60 days and 120 days. After freezing, the viability percentage and pollen tube length were determined. In vivo viability tests were performed through 24 pollinations involving different accessions. The results showed that the cryopreservation of pollen was efficient, attaining in vitro germination rates ranging from 8.24% to 94.25%, with average of 62.67% achieved 120 days after cryopreservation and pollen tube lengths longer than 0.23 mm after this same period. In the in vivo pollinations with cryopreserved grains, the seed formation rate was 70.83% for the different combinations. It was possible to cryopreserve pollen from all the pineapple accessions investigated after dehydration for 6 h on silica gel, with significant in vitro germination percentages and production of viable seeds. MenosCryopreservation of pollen is an additional way to conserve many species. In particular, it can be used as an alternative strategy to preserve the genetic variation of the genus Ananas. The aim of this study was to cryopreserve pollen from wild pineapple accessions to conserve a gene pool of the genus Ananas, as well as to minimize problems of asynchronous flowering among accessions preserved in a germplasm bank. Accessions of all botanical varieties were used to investigate different protocols for freezing pollen, as well as to determine their viability in vitro/in vivo during the investigation and after freezing in liquid nitrogen. Anthers with pollen of the different accessions were dehydrated and then immersed in liquid nitrogen for periods of 24 h, 60 days and 120 days. After freezing, the viability percentage and pollen tube length were determined. In vivo viability tests were performed through 24 pollinations involving different accessions. The results showed that the cryopreservation of pollen was efficient, attaining in vitro germination rates ranging from 8.24% to 94.25%, with average of 62.67% achieved 120 days after cryopreservation and pollen tube lengths longer than 0.23 mm after this same period. In the in vivo pollinations with cryopreserved grains, the seed formation rate was 70.83% for the different combinations. It was possible to cryopreserve pollen from all the pineapple accessions investigated after dehydration for 6 h on silica gel, with significant in... Mostrar Tudo |
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Thesagro: |
Abacaxi. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02070naa a2200181 a 4500
001 2081351
005 2017-12-04
008 2017 bl uuuu u00u1 u #d
100 1 $aSILVA, R. L. da
245 $aCryopreservation of pollen of wild pineapple accessions.$h[electronic resource]
260 $c2017
520 $aCryopreservation of pollen is an additional way to conserve many species. In particular, it can be used as an alternative strategy to preserve the genetic variation of the genus Ananas. The aim of this study was to cryopreserve pollen from wild pineapple accessions to conserve a gene pool of the genus Ananas, as well as to minimize problems of asynchronous flowering among accessions preserved in a germplasm bank. Accessions of all botanical varieties were used to investigate different protocols for freezing pollen, as well as to determine their viability in vitro/in vivo during the investigation and after freezing in liquid nitrogen. Anthers with pollen of the different accessions were dehydrated and then immersed in liquid nitrogen for periods of 24 h, 60 days and 120 days. After freezing, the viability percentage and pollen tube length were determined. In vivo viability tests were performed through 24 pollinations involving different accessions. The results showed that the cryopreservation of pollen was efficient, attaining in vitro germination rates ranging from 8.24% to 94.25%, with average of 62.67% achieved 120 days after cryopreservation and pollen tube lengths longer than 0.23 mm after this same period. In the in vivo pollinations with cryopreserved grains, the seed formation rate was 70.83% for the different combinations. It was possible to cryopreserve pollen from all the pineapple accessions investigated after dehydration for 6 h on silica gel, with significant in vitro germination percentages and production of viable seeds.
650 $aAbacaxi
700 1 $aSOUZA, E. H. de
700 1 $aVIEIRA, L. de J.
700 1 $aPELACANI, C. R.
700 1 $aSOUZA, F. V. D.
773 $tScientia Horticulturae$gv.219, p.326-334, May 2017.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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| Registros recuperados : 16 | |
| 7. |  | SILVA, R. L. DA; SOUZA, F. V. D.; FERREIRA, C. F.; COSTA, M. A. P. de C. Uso de marcadores ISSR na avaliação de estabilidade genética de plantas de abacaxi conservadas in vitro. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 3., 2014, Santos, SP. Resumos... Brasília, DF: Sociedade Brasileira de Recursos Genéticos, 2014.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 8. | | SOUZA, F. V. D.; SOUZA, E. H. de; KAYA, E.; VIEIRA, L. de J.; SILVA, R. L. da. Cryopreservation of pineapple Shoot Tips by the droplet vitrification technique. In: LOYOLA-VARGAS, V.r M; Ochoa-Alejo, N. (ed.). Plant Cell Culture Protocols, Methods in Molecular Biology. New York, NY: Humana Press, 2018. 269-277 v.1815. Cap. 18.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 11. | | SILVA, R. L. da; SOUZA, F. V. D.; FERREIRA, C. F.; COSTA, M. A. P. de C. Viabilidade, estabilidade genética e limpeza viral de plantas de abacaxizeiro mantidas sob conservação in vitro por dez anos. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 3., 2014, Santos, SP. Resumos... Brasília, DF: Sociedade Brasileira de Recursos Genéticos, 2014.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 15. | | SILVA, R. L. da; SOUZA, E. H. de; PELACANI, C. R.; JESUS, J. S. de; SOUZA, C. P. F.; SOUZA, A. B. P.; SOUZA, F. V. D. Validation of in vitro conservation of pineapple germplasm [Ananas comosus (L.) Merr.] for ten years based on field morphological characterization. Genetic Resources and Crop Evolution, v. 68, p.2051-206, 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 16. | | SILVA, R. L. da; FERREIRA, C. F.; LEDO, C. A. da S.; SOUZA, E. H. de; SILVA, P. H. da; COSTA, M. A. P. de C.; SOUZA, F. V. D. Viability and genetic stability of pineapple germplasm after 10 years of in vitro conservation. Plant Cell, Tissue and Organ Culture, v.127, p.123-133, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| Registros recuperados : 16 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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