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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
12/08/2010 |
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Data da última atualização: |
12/08/2010 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ROMEIRO, R. S.; LANNA FILHO, R.; MACAGNAN, D.; GARCIA, F. A. O.; SILVA, H. S. A. |
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Afiliação: |
Reginaldo da Silva Romeiro, UVF; Roberto Lanna Filho, UFV; Dirceu Macagnan, CEFET-RIO VERDE; Flávio Augusto de Oliveira Garcia, UFV; HARLLEN SANDRO ALVES SILVA, CNPMF. |
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Título: |
Evidence that the biocontrol agent Bacillus cereus synthesizes protein that can elicit increased resistance of tomato leaves to Corynespora cassiicola. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Tropical Plant Pathology, Brasília, DF, v. 35, n. 1, p. 11-15, jan./fev. 2010. |
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Idioma: |
Inglês |
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Conteúdo: |
Isolate UFV-101 of Bacillus cereus was selected in previous studies for promoting growth inducing resistance in plants. In a previous study, supernatant from cultures of the microorganism in a liquid medium was found to induce resistance in tomato foliage against the pathogens Pseudomonas syringae pv. tomato, Xanthomonas vesicatoria, Alternaria solani and Corynespora cassiicola. In the present work the microorganism was grown in a minimal medium for 48 h and the cells precipitated for centrifugation. The supernatant was concentrated by lyophilization, dialyzed in a 12 kDa cut-off point membrane and fractioned in column containing Sephadex G25 balanced in PBS buffer. The fractions corresponding to a protein peak were applied to tomato seedlings. After four days leaflets were collected and inoculated with the pathogen. C. cassiicola. The numbers of lesions produced by the pathogen on leaflets exposed to the bacterial supernatant were similar to those exposed to acibenzolar-S-methyl but fewer than in those treated with water. It is concluded that the supernatant contained protein which induced resistance in the tomato leaves against C. cassiicola. |
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Palavras-Chave: |
PGPR; Rhizobacteria. |
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Thesagro: |
Bactéria; Doença de Planta; Patógeno. |
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Thesaurus Nal: |
induced resistance. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 01915naa a2200241 a 4500
001 1859832
005 2010-08-12
008 2010 bl uuuu u00u1 u #d
100 1 $aROMEIRO, R. S.
245 $aEvidence that the biocontrol agent Bacillus cereus synthesizes protein that can elicit increased resistance of tomato leaves to Corynespora cassiicola.
260 $c2010
520 $aIsolate UFV-101 of Bacillus cereus was selected in previous studies for promoting growth inducing resistance in plants. In a previous study, supernatant from cultures of the microorganism in a liquid medium was found to induce resistance in tomato foliage against the pathogens Pseudomonas syringae pv. tomato, Xanthomonas vesicatoria, Alternaria solani and Corynespora cassiicola. In the present work the microorganism was grown in a minimal medium for 48 h and the cells precipitated for centrifugation. The supernatant was concentrated by lyophilization, dialyzed in a 12 kDa cut-off point membrane and fractioned in column containing Sephadex G25 balanced in PBS buffer. The fractions corresponding to a protein peak were applied to tomato seedlings. After four days leaflets were collected and inoculated with the pathogen. C. cassiicola. The numbers of lesions produced by the pathogen on leaflets exposed to the bacterial supernatant were similar to those exposed to acibenzolar-S-methyl but fewer than in those treated with water. It is concluded that the supernatant contained protein which induced resistance in the tomato leaves against C. cassiicola.
650 $ainduced resistance
650 $aBactéria
650 $aDoença de Planta
650 $aPatógeno
653 $aPGPR
653 $aRhizobacteria
700 1 $aLANNA FILHO, R.
700 1 $aMACAGNAN, D.
700 1 $aGARCIA, F. A. O.
700 1 $aSILVA, H. S. A.
773 $tTropical Plant Pathology, Brasília, DF$gv. 35, n. 1, p. 11-15, jan./fev. 2010.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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