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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
11/02/2008 |
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Data da última atualização: |
12/02/2008 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BRASILEIRO-VIDAL, A. C.; SANTOS-SEREJO, J. A. dos; SOARES FILHO, W. dos S.; GUERRA, M. |
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Afiliação: |
A. C. Brasileiro-Vidal, UFPE; Janay Almeida dos Santos-Serejo, CNPMF; Walter dos Santos Soares Filho, CNPMF; M. Guerra, UFPE. |
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Título: |
A simple chromosomal marker can reliably distinguishes Poncirus from Citrus species. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
Genetica, Netherlands, v. 129, n. 3, p. 273-279, mar. 2007. |
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Idioma: |
Português |
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Notas: |
DOI 10.1007/s10709-006-0007-4 |
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Conteúdo: |
Several chromosome types have been recognized in Citrus and related genera by chromomycin A3 (CMA) banding patterns and fluorescent in situ hybridization (FISH). They can be used to characterize cultivars and species or as markers in hybridization and backcrossing experiments. In the present work, characterization of six cultivars of P. trifoliata ("Barnes", "Fawcett", "Flying Dragon", "Pomeroy", "Rubidoux", "USDA") and one P. trifoliata × C. limonia hybrid was performed by sequential analyses of CMA banding and FISH using 5S and 45S rDNA as probes. All six cultivars showed a similar CMA+ banding pattern with the karyotype formula 4B + 8D + 6F. The capital letters indicate chromosomal types: B, a chromosome with one telomeric and one proximal band; D, with only one telomeric band; F, without bands. In situ hybridization labeling was also similar among cultivars. Three chromosome pairs displayed a closely linked set of 5S and 45S rDNA sites, two of them co-located with the proximal band of the B type chromosomes (B/5S-45S) and the third one co-located with the terminal band of a D pair (D/5S-45S). The B/5S-45S chromosome has never been found in any citrus accessions investigated so far. Therefore, this B chromosome can be used as a marker to recognize the intergeneric Poncirus × Citrus hybrids. The intergeneric hybrid analyzed here displayed the karyotype formula 4B + 8D + 6F, with two chromosome types B/5S-45S and two D/5S-45S. The karyotype formula and the presence of two B/5S-45S chromosomes clearly indicate that the plant investigated is a symmetric hybrid. It also demonstrates the suitability of karyotype analyses to differentiate zygotic embryos or somatic cell fusions involving trifoliate orange germplasm. MenosSeveral chromosome types have been recognized in Citrus and related genera by chromomycin A3 (CMA) banding patterns and fluorescent in situ hybridization (FISH). They can be used to characterize cultivars and species or as markers in hybridization and backcrossing experiments. In the present work, characterization of six cultivars of P. trifoliata ("Barnes", "Fawcett", "Flying Dragon", "Pomeroy", "Rubidoux", "USDA") and one P. trifoliata × C. limonia hybrid was performed by sequential analyses of CMA banding and FISH using 5S and 45S rDNA as probes. All six cultivars showed a similar CMA+ banding pattern with the karyotype formula 4B + 8D + 6F. The capital letters indicate chromosomal types: B, a chromosome with one telomeric and one proximal band; D, with only one telomeric band; F, without bands. In situ hybridization labeling was also similar among cultivars. Three chromosome pairs displayed a closely linked set of 5S and 45S rDNA sites, two of them co-located with the proximal band of the B type chromosomes (B/5S-45S) and the third one co-located with the terminal band of a D pair (D/5S-45S). The B/5S-45S chromosome has never been found in any citrus accessions investigated so far. Therefore, this B chromosome can be used as a marker to recognize the intergeneric Poncirus × Citrus hybrids. The intergeneric hybrid analyzed here displayed the karyotype formula 4B + 8D + 6F, with two chromosome types B/5S-45S and two D/5S-45S. The karyotype formula and the presence of two B... Mostrar Tudo |
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Palavras-Chave: |
Chromosome; CMA; rDNA. |
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Thesaurus Nal: |
Citrus; fish; Poncirus. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02411naa a2200241 a 4500
001 1654619
005 2008-02-12
008 2007 bl --- 0-- u #d
100 1 $aBRASILEIRO-VIDAL, A. C.
245 $aA simple chromosomal marker can reliably distinguishes Poncirus from Citrus species.
260 $c2007
500 $aDOI 10.1007/s10709-006-0007-4
520 $aSeveral chromosome types have been recognized in Citrus and related genera by chromomycin A3 (CMA) banding patterns and fluorescent in situ hybridization (FISH). They can be used to characterize cultivars and species or as markers in hybridization and backcrossing experiments. In the present work, characterization of six cultivars of P. trifoliata ("Barnes", "Fawcett", "Flying Dragon", "Pomeroy", "Rubidoux", "USDA") and one P. trifoliata × C. limonia hybrid was performed by sequential analyses of CMA banding and FISH using 5S and 45S rDNA as probes. All six cultivars showed a similar CMA+ banding pattern with the karyotype formula 4B + 8D + 6F. The capital letters indicate chromosomal types: B, a chromosome with one telomeric and one proximal band; D, with only one telomeric band; F, without bands. In situ hybridization labeling was also similar among cultivars. Three chromosome pairs displayed a closely linked set of 5S and 45S rDNA sites, two of them co-located with the proximal band of the B type chromosomes (B/5S-45S) and the third one co-located with the terminal band of a D pair (D/5S-45S). The B/5S-45S chromosome has never been found in any citrus accessions investigated so far. Therefore, this B chromosome can be used as a marker to recognize the intergeneric Poncirus × Citrus hybrids. The intergeneric hybrid analyzed here displayed the karyotype formula 4B + 8D + 6F, with two chromosome types B/5S-45S and two D/5S-45S. The karyotype formula and the presence of two B/5S-45S chromosomes clearly indicate that the plant investigated is a symmetric hybrid. It also demonstrates the suitability of karyotype analyses to differentiate zygotic embryos or somatic cell fusions involving trifoliate orange germplasm.
650 $aCitrus
650 $afish
650 $aPoncirus
653 $aChromosome
653 $aCMA
653 $arDNA
700 1 $aSANTOS-SEREJO, J. A. dos
700 1 $aSOARES FILHO, W. dos S.
700 1 $aGUERRA, M.
773 $tGenetica, Netherlands$gv. 129, n. 3, p. 273-279, mar. 2007.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Instrumentação. Para informações adicionais entre em contato com cnpdia.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Instrumentação. |
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Data corrente: |
23/02/2005 |
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Data da última atualização: |
18/01/2013 |
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Autoria: |
CRUVINEL, P. E.; CRESTANA, S.; ARTAXO, P.; MARTINS, J. V.; ARMELIN, M. J. A. |
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Título: |
Studying the spatial variability of Cr in agricultural field using both proton induced X-ray emission (PIXE) and neutron activation analysis (NAA) technique. |
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Ano de publicação: |
1995 |
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Fonte/Imprenta: |
In: INTERNATIONAL CONFERENCE ON PARTICLE INDUCED X-RAY EMISSION AND ITS ANALITICAL APPLICATION, 7., May, 1995, Padua, Italy. Program and abstracts book... Padua: [s.n.], 1995. p. 53. |
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Idioma: |
Português |
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Conteúdo: |
In the Field of soil physics, a technique which permits a non destructive, accurate and fast elemental analysis with a minimum of sample preparation effort is often desired. Although trace elements are minor components of the solid phase, they play an important role in soil fertility. Cr is of nutritional importance because it is required element in human and animal nutrition. The Ímmobiliiy of Cr may be responsible for an inadequate Cr supply to plants. This work demonstrates not only the suitability of PIXE as a fast and non-destructive technique useful to measure Cr content in soil samples but also a study of spatial variability of that element in agricultural field is outlined. To demonstrate the capabiliiy of the method soil samples were collected inside an agricultural field having 3500m2. The soil samples were analyzed using both PIXE and NAA technique. Besides, it was used Fourier spectral analysis to verify the distribution of Cr along of lhe sampled field. NAA were carried out by means of the y ray emitted by the photo peak of 51 Cr (320 keV). Results showed that there is a good linear relationship between the elemental concentration of Cr obtained using those techniques, i.e. a correlation coefficient of r2=O.82 was achieved. |
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Palavras-Chave: |
Cr; NAA; sistema PIXE; spatial variability. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02047naa a2200217 a 4500
001 1026847
005 2013-01-18
008 1995 bl --- 0-- u #d
100 1 $aCRUVINEL, P. E.
245 $aStudying the spatial variability of Cr in agricultural field using both proton induced X-ray emission (PIXE) and neutron activation analysis (NAA) technique.
260 $c1995
520 $aIn the Field of soil physics, a technique which permits a non destructive, accurate and fast elemental analysis with a minimum of sample preparation effort is often desired. Although trace elements are minor components of the solid phase, they play an important role in soil fertility. Cr is of nutritional importance because it is required element in human and animal nutrition. The Ímmobiliiy of Cr may be responsible for an inadequate Cr supply to plants. This work demonstrates not only the suitability of PIXE as a fast and non-destructive technique useful to measure Cr content in soil samples but also a study of spatial variability of that element in agricultural field is outlined. To demonstrate the capabiliiy of the method soil samples were collected inside an agricultural field having 3500m2. The soil samples were analyzed using both PIXE and NAA technique. Besides, it was used Fourier spectral analysis to verify the distribution of Cr along of lhe sampled field. NAA were carried out by means of the y ray emitted by the photo peak of 51 Cr (320 keV). Results showed that there is a good linear relationship between the elemental concentration of Cr obtained using those techniques, i.e. a correlation coefficient of r2=O.82 was achieved.
653 $aCr
653 $aNAA
653 $asistema PIXE
653 $aspatial variability
700 1 $aCRESTANA, S.
700 1 $aARTAXO, P.
700 1 $aMARTINS, J. V.
700 1 $aARMELIN, M. J. A.
773 $tIn: INTERNATIONAL CONFERENCE ON PARTICLE INDUCED X-RAY EMISSION AND ITS ANALITICAL APPLICATION, 7., May, 1995, Padua, Italy. Program and abstracts book... Padua: [s.n.], 1995. p. 53.
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Embrapa Instrumentação (CNPDIA) |
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