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| Registros recuperados : 19 | |
| 7. |  | MATTOS, L. A.; AMORIM, E. P.; COHEN, K. de O.; AMORIM, T. B. de; SILVA, S. de O. e. Agronomic, physical and chemical characterization of banana fruits. Crop Breeding and Applied Biotechnology, Londrina, v. 10, n. 3, p. 225-231, set. 2010.| Biblioteca(s): Embrapa Cerrados; Embrapa Mandioca e Fruticultura. |
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| 8. | | MEIRA, C. F.; MARTINS, F. M.; MATTOS, L. A.; AMORIM, E. P.; LEDO, C. A. da S.; FERREIRA, C. F. Histological characterization of banana (Musa Spp.) genotypes submitted to water deficit. In: INTERNATIONAL HORTICULTURAL CONGRESS, 29., 2014, Brisbane. Sustaining lives, livelihoods and landscapes. [Abstracts...]. Brisbane: AuSHS : ISHS, 2014.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 9. | | AMORIM, E. P.; MATTOS, L. A; AMORIM, V. B. O; TAMYRES, A. B; COHEN, K. de O.; SILVA, S. O. Genetic diversity between accessions of banana through microsatellite markers. In: INTERNATIONAL HORTICULTURAL CONGRESS, 28., 2010, Lisboa. Science and horticulture for people: programme & book of abstracts. Lisboa: ISHS, 2010. 1 CD-ROM. 2 p. 747 S18.004| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 10. | | MATTOS, L. A.; AMORIM, E. P.; COHEN, K. de O.; SILVA, S. de O. e; AMORIM, T. B. do. Caracterização físico-química de genótipos de bananeira. In: ENCONTRO DA REDE DE RECURSOS GENÉTICOS VEGETAIS DO ESTADO DA BAHIA, 3.; SIMPÓSIO DE RECURSOS GENÉTICOS DE PLANTAS CULTIVADAS NO NORDESTE BRASILEIRO, 2., 2008, Vitória da Conquista. [Anais]... Vitória da Conquista: Universidade Estadual do Sudoeste da Bahia, 2008.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 11. | | MATTOS, L. A.; AMORIM, E. P.; COHEN, K. de O.; SILVA, S. de O. e; AMORIM, T. B. do. Variabilidade genética entre acessos de bananeira com teores variáveis de compostos funcionais. In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 21., 2010, Natal. Frutas: saúde, inovação e responsabilidade: anais. Natal: Sociedade Brasileira de Fruticultura, 2010. pdf 1870| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 12. | | AMORIM, E. P.; MATTOS, L. A; AMORIM, V. B.; TAMYRES, A. B.; COHEN, K. de O.; SILVA, S. O; LEDO, C. A. da S. Agronomical and molecular characterization of banana accessions. In: INTERNATIONAL HORTICULTURAL CONGRESS, 28., 2010, Lisboa. Science and horticulture for people: programme & book of abstracts. Lisboa: ISHS, 2010. 1 CD-ROM. v.2 p. 749 PDF. S18.010.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 13. | | MATTOS, L. A.; AMORIM, E. P.; AMORIM, V. B. de O.; COHEN, K. de O.; LEDO, C. A. da S.; SILVA, S. de O. e. Agronomical and molecular characterization of banana germplasm. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 45, n. 2, p. 146-154, fev. 2010.| Biblioteca(s): Embrapa Mandioca e Fruticultura; Embrapa Unidades Centrais. |
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| 14. | | SOARES FILHO, W. dos S.; LEDO, C. A. da S.; PASSOS, O. S.; SOUZA, A. da S.; MATTOS, L. A.; QUINTELA, M. P. Parentais femininos monoembriônicos na obtenção de porta-enxertos híbridos de citros. Revista Brasileira de Fruticultura, Jaboticabal, v. 30, n. 1, p. 215-218, mar. 2008.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 15. | | SOARES FILHO, W. dos S.; LEDO, C. A. da S.; QUINTELA, M. P.; MATTOS, L. A.; PASSOS, O. S.; SOUZA, A. da S. Cruzamentos em citros: frequência e vigor de híbridos. Revista Brasileira de Fruticultura, Jaboticabal, v. 29, n. 2, p. 393-398, ago. 2007.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 16. | | SOARES FILHO, W. dos S.; LEDO, C. A. da S.; SOUZA, A. da S.; PASSOS, O. S.; QUINTELA, M. P.; MATTOS, L. A. Potencial de obtenção de novos porta-enxertos em cruzamentos envolvendo limoeiro 'Cravo', laranjeira 'Azeda', tangerineira 'Sunki' e híbridos de Poncirus Trifoliata. Revista Brasileira de Fruticultura, Jaboticabal, v. 30, n. 1, p. 223-228, mar. 2008.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 17. | | MATTOS, L. A.; AMORIM, T. B. do; FERREIRA, C. F.; LEDO, C. A. da S.; AMORIM, E. P.; COELHO, M. F.; PEREIRA, V. M. Variação da condutância estomática em variedades comerciais de bananeira (musa spp.). In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 21., 2010, Natal. Frutas: saúde, inovação e responsabilidade: anais. Natal: Sociedade Brasileira de Fruticultura, 2010. pdf 1869| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 19. | | AMORIM, E. P.; COHEN, K. de O.; AMORIM, V. B. de O.; MATTOS, L. A.; SANTOS-SEREJO, J. A. dos; SILVA, S. de O. e; PAES, N. S.; MONTE, D. C. Conteúdo de vitamina C e carotenóides totais em bananeira. In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 20.; ANNUAL MEETING OF THE INTERAMERICAN SOCIETY FOR TROPICAL HORTICULTURE, 54., 2008, Vitória. Frutas para todos: estratégias, tecnologias e visão sustentável: anais. Vitória: INCAPER: Sociedade Brasileira de Fruticultura, 2008.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| Registros recuperados : 19 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Cerrados. Para informações adicionais entre em contato com cpac.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Cerrados; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
11/12/2020 |
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Data da última atualização: |
11/12/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
MARQUES, T. C.; SANTOS, E. C. da S.; DIESEL, T. O.; MARTINS, C. F.; CUMPA, H. C. B.; LEME, L. de O.; DODE, M. A. N.; ALVES, B. G.; COSTA, F. P. H.; OLIVEIRA, E. B.; GAMBARINI, M. L. |
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Afiliação: |
CARLOS FREDERICO MARTINS, CPAC; MARGOT ALVES NUNES DODE, Cenargen. |
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Título: |
Blastocoel fluid removal and melatonin supplementation in the culture medium improve the viability of vitrified bovine embryos. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Theriogenology, Vv. 160, p. 134-141, 2021. |
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Páginas: |
p. 134-141 |
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ISSN: |
0093-691X |
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Idioma: |
Português |
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Conteúdo: |
In this study, we investigated the effects of melatonin supplementation in the culture medium and blastocoel fluid removal (BFR) before vitrification on the quality and viability of in vitro-derived bovine embryos. After fertilization, presumptive zygotes were assigned to one of the following treatments: control, in vitro standard culture (IVC) medium; IVC þ M109 , IVC medium supplemented 109 M melatonin; or IVC þ M109 BFR, IVC medium supplemented with 109 M melatonin plus BFR on day 7 (D7) of culture. D7 blastocysts were vitrified by the Cryotop method and, after 5 mo of storage, were warmed and incubated for an additional 72 h. The re-expansion rate was evaluated after 2 and 24 h, and the hatching rate was evaluated after 24, 48, and 72 h. At 72 h, the total number of cells (TNC); number of apoptotic cells (NAC); and expression of genes related to oxidative stress (HSPA5), cell metabolism (SLC2A3), cell repair (MSH6), placentation (KRT8 and PLAC8), and implantation (FOSL1) were assessed in the blastocysts. Less than 30% of the control blastocysts re-expanded until 2 h, whereas more than 85% of the IVC þ M109 and IVC þ M109 BFR blastocysts re-expanded (P < 0.05). The hatching rate of IVC þ M109 BFR blastocysts increased at all time points (P < 0.05), reaching 66.8% at 72 h of incubation. The TNC was similar among treatments (P > 0.05), regardless of vitrification/warming and re-cultivation. The NAC:TNC was smaller for melatonin-treated blastocysts (P < 0.05). BFR increased HSPA5 (P ¼ 0.0118) expression and did not affect SLC2A3, MSH6, KRT8, and FOSL1 expression (P > 0.05). In conclusion, melatonin (109 M) supplementation in the culture medium and BFR on D7 of culture increased the hatching rate 24, 48, and 72 h after warming of the vitrified embryos, indicating an improvement in cryotolerance. MenosIn this study, we investigated the effects of melatonin supplementation in the culture medium and blastocoel fluid removal (BFR) before vitrification on the quality and viability of in vitro-derived bovine embryos. After fertilization, presumptive zygotes were assigned to one of the following treatments: control, in vitro standard culture (IVC) medium; IVC þ M109 , IVC medium supplemented 109 M melatonin; or IVC þ M109 BFR, IVC medium supplemented with 109 M melatonin plus BFR on day 7 (D7) of culture. D7 blastocysts were vitrified by the Cryotop method and, after 5 mo of storage, were warmed and incubated for an additional 72 h. The re-expansion rate was evaluated after 2 and 24 h, and the hatching rate was evaluated after 24, 48, and 72 h. At 72 h, the total number of cells (TNC); number of apoptotic cells (NAC); and expression of genes related to oxidative stress (HSPA5), cell metabolism (SLC2A3), cell repair (MSH6), placentation (KRT8 and PLAC8), and implantation (FOSL1) were assessed in the blastocysts. Less than 30% of the control blastocysts re-expanded until 2 h, whereas more than 85% of the IVC þ M109 and IVC þ M109 BFR blastocysts re-expanded (P < 0.05). The hatching rate of IVC þ M109 BFR blastocysts increased at all time points (P < 0.05), reaching 66.8% at 72 h of incubation. The TNC was similar among treatments (P > 0.05), regardless of vitrification/warming and re-cultivation. The NAC:TNC was smaller for melatonin-treated blastocysts (P < 0.05). BFR increased ... Mostrar Tudo |
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Palavras-Chave: |
Apoptose celular; Criotolerância; Fertilização; Melatonina; Vitrificação. |
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Thesagro: |
Cultura de Embrião; Embrião. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02843naa a2200349 a 4500
001 2127986
005 2020-12-11
008 2021 bl uuuu u00u1 u #d
022 $a0093-691X
100 1 $aMARQUES, T. C.
245 $aBlastocoel fluid removal and melatonin supplementation in the culture medium improve the viability of vitrified bovine embryos.$h[electronic resource]
260 $c2021
300 $ap. 134-141
520 $aIn this study, we investigated the effects of melatonin supplementation in the culture medium and blastocoel fluid removal (BFR) before vitrification on the quality and viability of in vitro-derived bovine embryos. After fertilization, presumptive zygotes were assigned to one of the following treatments: control, in vitro standard culture (IVC) medium; IVC þ M109 , IVC medium supplemented 109 M melatonin; or IVC þ M109 BFR, IVC medium supplemented with 109 M melatonin plus BFR on day 7 (D7) of culture. D7 blastocysts were vitrified by the Cryotop method and, after 5 mo of storage, were warmed and incubated for an additional 72 h. The re-expansion rate was evaluated after 2 and 24 h, and the hatching rate was evaluated after 24, 48, and 72 h. At 72 h, the total number of cells (TNC); number of apoptotic cells (NAC); and expression of genes related to oxidative stress (HSPA5), cell metabolism (SLC2A3), cell repair (MSH6), placentation (KRT8 and PLAC8), and implantation (FOSL1) were assessed in the blastocysts. Less than 30% of the control blastocysts re-expanded until 2 h, whereas more than 85% of the IVC þ M109 and IVC þ M109 BFR blastocysts re-expanded (P < 0.05). The hatching rate of IVC þ M109 BFR blastocysts increased at all time points (P < 0.05), reaching 66.8% at 72 h of incubation. The TNC was similar among treatments (P > 0.05), regardless of vitrification/warming and re-cultivation. The NAC:TNC was smaller for melatonin-treated blastocysts (P < 0.05). BFR increased HSPA5 (P ¼ 0.0118) expression and did not affect SLC2A3, MSH6, KRT8, and FOSL1 expression (P > 0.05). In conclusion, melatonin (109 M) supplementation in the culture medium and BFR on D7 of culture increased the hatching rate 24, 48, and 72 h after warming of the vitrified embryos, indicating an improvement in cryotolerance.
650 $aCultura de Embrião
650 $aEmbrião
653 $aApoptose celular
653 $aCriotolerância
653 $aFertilização
653 $aMelatonina
653 $aVitrificação
700 1 $aSANTOS, E. C. da S.
700 1 $aDIESEL, T. O.
700 1 $aMARTINS, C. F.
700 1 $aCUMPA, H. C. B.
700 1 $aLEME, L. de O.
700 1 $aDODE, M. A. N.
700 1 $aALVES, B. G.
700 1 $aCOSTA, F. P. H.
700 1 $aOLIVEIRA, E. B.
700 1 $aGAMBARINI, M. L.
773 $tTheriogenology, Vv. 160, p. 134-141, 2021.
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