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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
25/11/2011 |
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Data da última atualização: |
26/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GUTIÉRREZ, I. E. M.; NEPOMUCENO, C. F.; LEDO, C. A. da S.; SANTANA, J. R. F. |
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Afiliação: |
I. E. M. GUTIÉRREZ, UEFS; C. F. NEPOMUCENO, UEFS; CARLOS ALBERTO DA SILVA LEDO, CNPMF; J. R. F. SANTANA, UEFS. |
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Título: |
Micropropagation and acclimatization of Bauhinia cheilantha (an important medicinal plant). |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
African Journal of Biotechnology, v. 10, n. 8, p. 1353-1358, February, 2011. |
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ISSN: |
1684-5315 © 2011 |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of this study is to establish a micropropagation protocol for Bauhinia cheilantha. This was undertaken through an evaluation of 6-benzylaminopurine (BAP) effects, alone or in combination with a-napthaleneacetic acid (NAA), on the morphogenesis of different explants, taken from 20 day old seedlings grown in vitro. In the rooting, different auxins, indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) and NAA were tested under the influence of activated charcoal. The nodal segments showed an organogenic capacity which is 2.4 times greater than the cotyledonary segment. The highest number of shoots (2.84) was obtained at 2.0 mgl-1 BAP. The use of 0.5 mgl-1 IAA, 1.0 mgl-1 NAA or 0.25 mgl-1 IBA gave the highest percentage of rooting (about 65.0%). Concerning the activated charcoal (AC), there was a beneficial effect in most of the analyzed characteristics at a concentration of 0.5 gl-1. The culture tube, with forced ventilation during rooting, increased the percentage of seedling survival during the acclimatization in greenhouse. This finding reveals that it is possible to obtain in vitro culture of B. cheilantha using the segment nodal as a source of explants in woody plant medium (WPM) supplemented with BAP. |
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Palavras-Chave: |
Activated charcoal; Bauhinia cheilantha; Seedling node. |
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Thesaurus Nal: |
in vitro culture; organogenesis. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 01961naa a2200229 a 4500
001 1907258
005 2023-05-26
008 2011 bl uuuu u00u1 u #d
022 $a1684-5315 © 2011
100 1 $aGUTIÉRREZ, I. E. M.
245 $aMicropropagation and acclimatization of Bauhinia cheilantha (an important medicinal plant).$h[electronic resource]
260 $c2011
520 $aThe objective of this study is to establish a micropropagation protocol for Bauhinia cheilantha. This was undertaken through an evaluation of 6-benzylaminopurine (BAP) effects, alone or in combination with a-napthaleneacetic acid (NAA), on the morphogenesis of different explants, taken from 20 day old seedlings grown in vitro. In the rooting, different auxins, indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) and NAA were tested under the influence of activated charcoal. The nodal segments showed an organogenic capacity which is 2.4 times greater than the cotyledonary segment. The highest number of shoots (2.84) was obtained at 2.0 mgl-1 BAP. The use of 0.5 mgl-1 IAA, 1.0 mgl-1 NAA or 0.25 mgl-1 IBA gave the highest percentage of rooting (about 65.0%). Concerning the activated charcoal (AC), there was a beneficial effect in most of the analyzed characteristics at a concentration of 0.5 gl-1. The culture tube, with forced ventilation during rooting, increased the percentage of seedling survival during the acclimatization in greenhouse. This finding reveals that it is possible to obtain in vitro culture of B. cheilantha using the segment nodal as a source of explants in woody plant medium (WPM) supplemented with BAP.
650 $ain vitro culture
650 $aorganogenesis
653 $aActivated charcoal
653 $aBauhinia cheilantha
653 $aSeedling node
700 1 $aNEPOMUCENO, C. F.
700 1 $aLEDO, C. A. da S.
700 1 $aSANTANA, J. R. F.
773 $tAfrican Journal of Biotechnology$gv. 10, n. 8, p. 1353-1358, February, 2011.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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| Registros recuperados : 4 | |
| 3. |  | GUTIÉRREZ, I. E. M. de; CAMPOS, V. C. A.; NEPOMUCENO, C. F.; LEDO, C. A. da S.; SANTANA, J. R. F. de. Multiplicação de Bauhinia cheilantha (Bong.) Steud. (Fabaceae). In: CONGRESSO BRASILEIRO DE FLORICULTURA E PLANTAS ORNAMENTAIS, 17.; CONGRESSO BRASILEIRO DE CULTURA DE TECIDOS DE PLANTAS, 4.; 2009, Aracaju. Ciência, inovação e sustentabilidade: [anais...]. Aracaju: Embrapa Tabuleiros Costeiros; Cruz das Almas: Embrapa Mandioca e Fruticultura Tropical, 2009. 1 CD-ROM. (Embrapa Tabuleiros Costeiros. Documentos, 150). Organizadores: Ana da Silva Lédo; Fernanda Vidigal D. Souza; Vivian Loges; Everton Hilo de Souza; Ana Cecília R. de Castro.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 4. | | GUTIÉRREZ, I. E. M. de; CAMPOS, V. C. A.; NEPOMUCENO, J. R. F. de S.; LEDO, C. A. da S.; SANTANA, J. R. F. de. Multiplicação de Tapirira guianensis Aubl. (Anacardiaceae). In: CONGRESSO BRASILEIRO DE FLORICULTURA E PLANTAS ORNAMENTAIS, 17.; CONGRESSO BRASILEIRO DE CULTURA DE TECIDOS DE PLANTAS, 4.; 2009, Aracaju. Ciência, inovação e sustentabilidade: [anais...].Aracaju: Embrapa Tabuleiros Costeiros; Cruz das Almas: Embrapa Mandioca e Fruticultura Tropical, 2009. 1 CD-ROM. (Embrapa Tabuleiros Costeiros. Documentos, 150). Organizadores: Ana da Silva Lédo; Fernanda Vidigal D. Souza; Vivian Loges; Everton Hilo de Souza; Ana Cecília R. de Castro.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| Registros recuperados : 4 | |
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