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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
29/12/2009 |
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Data da última atualização: |
27/01/2010 |
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Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
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Autoria: |
SANTOS, K. C. dos; ANDRADE, E. C. de. |
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Afiliação: |
Keila Cidreira dos Santos, FAMAM; Eduardo Chumbinho de Andrade, CNPMF. |
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Título: |
Detecção do vírus que causa a murcha do abacaxi por meio de RT-PCR, utilizando primers específicos e degenerados. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
In: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA TROPICAL, 3., 2009, Cruz das Almas. Anais... Cruz das Almas: Embrapa Mandioca e Fruticultura Tropical, 2009. 1 CD-ROM. |
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Idioma: |
Português |
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Conteúdo: |
O abacaxizeiro (Ananas comosus var. comosus), por ser de propagação vegetativa, possui a vantagem da multiplicação clonal do material de plantio pela utilização de brotações gerados em cada planta. Entretanto, a propagação vegetativa favorece a disseminação de doenças, como o complexo de vírus associado à murcha do abacaxizeiro (Pineapple mealybug associated virus, PMWaV). Além dos danos diretos, existe uma grande dificuldade de se detectar o vírus em mudas comercializadas, acarretando na maior disseminação/introdução do vírus. A detecção do vírus pode ser feita por meio da técnica de RT-PCR, utilizando-se oligonucleotídeos específicos para os tipos de PMWaV. Entretanto, é interessante que se aprimore a metodologia, de forma a obter uma indexação simultânea dos três vírus, para gerar uma economia de tempo e dinheiro. Para isso, foram desenhados oligonucleotídeos degenerados, baseados no alinhamento das sequências do PMWaV-1 e 2, e utilizados para a detecção dos vírus em plantas de abacaxi. Os resultados demonstram que é possível a detecção dos vírus, tanto com os oligonucleotídeos específicos quanto com os degenerados. |
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Palavras-Chave: |
Ananas comosus var; comosus; Pineapple mealybug associated virus; Vírus da murcha. |
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Thesagro: |
Abacaxi; Muda. |
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Categoria do assunto: |
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Marc: |
LEADER 01924naa a2200205 a 4500
001 1656518
005 2010-01-27
008 2009 bl uuuu u00u1 u #d
100 1 $aSANTOS, K. C. dos
245 $aDetecção do vírus que causa a murcha do abacaxi por meio de RT-PCR, utilizando primers específicos e degenerados.
260 $c2009
520 $aO abacaxizeiro (Ananas comosus var. comosus), por ser de propagação vegetativa, possui a vantagem da multiplicação clonal do material de plantio pela utilização de brotações gerados em cada planta. Entretanto, a propagação vegetativa favorece a disseminação de doenças, como o complexo de vírus associado à murcha do abacaxizeiro (Pineapple mealybug associated virus, PMWaV). Além dos danos diretos, existe uma grande dificuldade de se detectar o vírus em mudas comercializadas, acarretando na maior disseminação/introdução do vírus. A detecção do vírus pode ser feita por meio da técnica de RT-PCR, utilizando-se oligonucleotídeos específicos para os tipos de PMWaV. Entretanto, é interessante que se aprimore a metodologia, de forma a obter uma indexação simultânea dos três vírus, para gerar uma economia de tempo e dinheiro. Para isso, foram desenhados oligonucleotídeos degenerados, baseados no alinhamento das sequências do PMWaV-1 e 2, e utilizados para a detecção dos vírus em plantas de abacaxi. Os resultados demonstram que é possível a detecção dos vírus, tanto com os oligonucleotídeos específicos quanto com os degenerados.
650 $aAbacaxi
650 $aMuda
653 $aAnanas comosus var
653 $acomosus
653 $aPineapple mealybug associated virus
653 $aVírus da murcha
700 1 $aANDRADE, E. C. de
773 $tIn: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA TROPICAL, 3., 2009, Cruz das Almas. Anais... Cruz das Almas: Embrapa Mandioca e Fruticultura Tropical, 2009. 1 CD-ROM.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Florestas. Para informações adicionais entre em contato com cnpf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
22/09/2008 |
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Data da última atualização: |
22/09/2008 |
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Autoria: |
BISPO, A.; CREAMER, R.; DOMBOS, M.; FUCHS, M.; GRAEFE, U.; SOUSA, J. P.; PERES, G.; ROMBKE, J.; RUTGERS, M.; WINDING, A. |
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Título: |
Monitoring the decline of soil biodiversity in European soils. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: INTERNATIONAL COLLOQUIUM ON SOIL ZOOLOGY, 15; INTERNATIONAL COLLOQUIUM ON APTERYGOTA, 12., 2008, Curitiba. Biodiversity, conservation and sustainabele management of soil animal: abstracts. Colombo: Embrapa Florestas. Editors: George Gardner Brown; Klaus Dieter Sautter; Renato Marques; Amarildo Pasini. 1 CD-ROM. |
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Idioma: |
Inglês |
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Conteúdo: |
The soil biota have important fundamental roles in soil processes and contribute both directly
and indirectly to many important ecosystem functions such as nutrient cycling, soil structure,
decomposition of organic matter. It is essential to understand the importance of species
diversity in soil and their impact upon soil quality and function. The EU project ENVASSO
(Environmental Assessment of Soil for Monitoring) addressed the 8 threats to soil identified
by the Commission (CEC, 2002). The aim of the project was to design and test a single,
integrated and operational set of EU-wide criteria and indicators that will provide a basis for
a harmonised comprehensive soil and land information system for monitoring in Europe. To
identify suitable indicators for monitoring the decline in soil biodiversity, indicators were
selected both from a literature review and an inventory of national monitoring EU programmes.
Within this project decline in soil biodiversity was defined as the reduction of forms of life
living in soils (both in terms of quantity and variety) and of related functions, causing a
deterioration or loss of one or more soil functions. Whereas the literature review allows the
identification of about 100 possible indicators the inventory of existing monitoring networks
shows that few indicators are really measured. For monitoring application it was considered
that only 3 key indicators per soil stress were practical, however this was considered a
difficult task for indicating biodiversity decline due to the complexity of soil biota and multifunctionality
in soils. Therefore stringent criteria were applied to the selection process to
evaluate: 1) methodology standardisation, 2) complementarity to other indicators, and 3)
interpretation at both scientific and policy levels. The key indicators selected were chosen
as representative of 3 functional levels in soil: a) abundance, biomass and species diversity
of Earthworms ? macrofauna, b) abundance and species diversity of Collembola ? mesofauna
and c) microbial respiration. Procedures and protocols were written based upon current ISO
standards and adapted for assessment at a European scale. Pilot sites were then established
in 4 countries (France, Ireland, Portugal and Hungary) to test the ease of measurement of
the selected indicators and their efficiency to indicate the decline in soil biodiversity. The
effectiveness of each indicator and their sensitivity to detect change across a range of landuse
categories at a European scale will be discussed using data from these sites. MenosThe soil biota have important fundamental roles in soil processes and contribute both directly
and indirectly to many important ecosystem functions such as nutrient cycling, soil structure,
decomposition of organic matter. It is essential to understand the importance of species
diversity in soil and their impact upon soil quality and function. The EU project ENVASSO
(Environmental Assessment of Soil for Monitoring) addressed the 8 threats to soil identified
by the Commission (CEC, 2002). The aim of the project was to design and test a single,
integrated and operational set of EU-wide criteria and indicators that will provide a basis for
a harmonised comprehensive soil and land information system for monitoring in Europe. To
identify suitable indicators for monitoring the decline in soil biodiversity, indicators were
selected both from a literature review and an inventory of national monitoring EU programmes.
Within this project decline in soil biodiversity was defined as the reduction of forms of life
living in soils (both in terms of quantity and variety) and of related functions, causing a
deterioration or loss of one or more soil functions. Whereas the literature review allows the
identification of about 100 possible indicators the inventory of existing monitoring networks
shows that few indicators are really measured. For monitoring application it was considered
that only 3 key indicators per soil stress were practical, however this was considered a
difficult task for in... Mostrar Tudo |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03419naa a2200229 a 4500
001 1314890
005 2008-09-22
008 2008 bl uuuu u00u1 u #d
100 1 $aBISPO, A.
245 $aMonitoring the decline of soil biodiversity in European soils.
260 $c2008
520 $aThe soil biota have important fundamental roles in soil processes and contribute both directly and indirectly to many important ecosystem functions such as nutrient cycling, soil structure, decomposition of organic matter. It is essential to understand the importance of species diversity in soil and their impact upon soil quality and function. The EU project ENVASSO (Environmental Assessment of Soil for Monitoring) addressed the 8 threats to soil identified by the Commission (CEC, 2002). The aim of the project was to design and test a single, integrated and operational set of EU-wide criteria and indicators that will provide a basis for a harmonised comprehensive soil and land information system for monitoring in Europe. To identify suitable indicators for monitoring the decline in soil biodiversity, indicators were selected both from a literature review and an inventory of national monitoring EU programmes. Within this project decline in soil biodiversity was defined as the reduction of forms of life living in soils (both in terms of quantity and variety) and of related functions, causing a deterioration or loss of one or more soil functions. Whereas the literature review allows the identification of about 100 possible indicators the inventory of existing monitoring networks shows that few indicators are really measured. For monitoring application it was considered that only 3 key indicators per soil stress were practical, however this was considered a difficult task for indicating biodiversity decline due to the complexity of soil biota and multifunctionality in soils. Therefore stringent criteria were applied to the selection process to evaluate: 1) methodology standardisation, 2) complementarity to other indicators, and 3) interpretation at both scientific and policy levels. The key indicators selected were chosen as representative of 3 functional levels in soil: a) abundance, biomass and species diversity of Earthworms ? macrofauna, b) abundance and species diversity of Collembola ? mesofauna and c) microbial respiration. Procedures and protocols were written based upon current ISO standards and adapted for assessment at a European scale. Pilot sites were then established in 4 countries (France, Ireland, Portugal and Hungary) to test the ease of measurement of the selected indicators and their efficiency to indicate the decline in soil biodiversity. The effectiveness of each indicator and their sensitivity to detect change across a range of landuse categories at a European scale will be discussed using data from these sites.
700 1 $aCREAMER, R.
700 1 $aDOMBOS, M.
700 1 $aFUCHS, M.
700 1 $aGRAEFE, U.
700 1 $aSOUSA, J. P.
700 1 $aPERES, G.
700 1 $aROMBKE, J.
700 1 $aRUTGERS, M.
700 1 $aWINDING, A.
773 $tIn: INTERNATIONAL COLLOQUIUM ON SOIL ZOOLOGY, 15; INTERNATIONAL COLLOQUIUM ON APTERYGOTA, 12., 2008, Curitiba. Biodiversity, conservation and sustainabele management of soil animal: abstracts. Colombo: Embrapa Florestas. Editors: George Gardner Brown; Klaus Dieter Sautter; Renato Marques; Amarildo Pasini. 1 CD-ROM.
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