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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
19/09/2019 |
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Data da última atualização: |
06/12/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FERREIRA, C. F.; GUTIERREZ, D. L.; KREUZE, J. F.; ISKRA-CARUANA, M. L.; CHABANNES, M.; BARBOSA, A. C. O.; SANTOS, T. A.; SILVA, A. G. S.; SANTOS, R. M. F.; AMORIM, E. P.; OLIVEIRA, S. A. S. de; JESUS, O. N. de. |
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Afiliação: |
CLAUDIA FORTES FERREIRA, CNPMF; D. L. GUTIERREZ, INIA; J. F. KREUZE, CIP; M. L. ISKRA-CARUANA, CIRAD; M. CHABANNES, CIRAD; A. C. O. BARBOSA, UFRB; T. A. SANTOS, UFRB; A. G. S. SILVA, UFRB; R.M.F. SANTOS, UEFS; EDSON PERITO AMORIM, CNPMF; SAULO ALVES SANTOS DE OLIVEIRA, CNPMF; ONILDO NUNES DE JESUS, CNPMF. |
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Título: |
Rapid plant DNA and RNA extraction protocol using a bench drill Brief Note. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 18, n.3, 2019. |
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Idioma: |
Inglês |
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Notas: |
gmr18394 |
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Conteúdo: |
Plant DNA and RNA extraction methods are well established, with a wide range of protocols, depending on the purposes of each laboratory/research. Nowadays, quick, inexpensive and easy plant DNA and RNA extraction methods are highly sought after. We developed an optimized protocol for plant DNA and RNA extraction that uses an inexpensive bench drill and plastic bags and does not require liquid nitrogen. DNA from leaves and RNA from leaves and roots of banana, pineapple, citrus, papaya, passion fruit and cassava, were extracted using a basic cetyltrimethylammonium bromide method. Both nucleic acids were quantified and evaluated for quality based on agarose gel electrophoresis. The DNA and RNA extractions were successful for all species, and RNA quality in pellets was maintained after storage at room temperature for three weeks. This protocol can reduce costs considerably in laboratories with ongoing routine activities of DNA and RNA extraction for genetic diversity and gene expression analyses, where other conventional methods have not been successful due to explant, condition of samples and quantity and quality of nucleic acids. This is especially relevant for many laboratories in developing countries where the cost and availability of liquid nitrogen may be a constraint. |
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Thesagro: |
Melhoramento Vegetal. |
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Thesaurus Nal: |
Breeding and Genetic Improvement. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/202165/1/-rapid-plant-dna-and-rna-extraction-protocol-using-bench-drill.pdf
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Marc: |
LEADER 02135naa a2200289 a 4500
001 2112332
005 2019-12-06
008 2019 bl uuuu u00u1 u #d
100 1 $aFERREIRA, C. F.
245 $aRapid plant DNA and RNA extraction protocol using a bench drill Brief Note.$h[electronic resource]
260 $c2019
500 $agmr18394
520 $aPlant DNA and RNA extraction methods are well established, with a wide range of protocols, depending on the purposes of each laboratory/research. Nowadays, quick, inexpensive and easy plant DNA and RNA extraction methods are highly sought after. We developed an optimized protocol for plant DNA and RNA extraction that uses an inexpensive bench drill and plastic bags and does not require liquid nitrogen. DNA from leaves and RNA from leaves and roots of banana, pineapple, citrus, papaya, passion fruit and cassava, were extracted using a basic cetyltrimethylammonium bromide method. Both nucleic acids were quantified and evaluated for quality based on agarose gel electrophoresis. The DNA and RNA extractions were successful for all species, and RNA quality in pellets was maintained after storage at room temperature for three weeks. This protocol can reduce costs considerably in laboratories with ongoing routine activities of DNA and RNA extraction for genetic diversity and gene expression analyses, where other conventional methods have not been successful due to explant, condition of samples and quantity and quality of nucleic acids. This is especially relevant for many laboratories in developing countries where the cost and availability of liquid nitrogen may be a constraint.
650 $aBreeding and Genetic Improvement
650 $aMelhoramento Vegetal
700 1 $aGUTIERREZ, D. L.
700 1 $aKREUZE, J. F.
700 1 $aISKRA-CARUANA, M. L.
700 1 $aCHABANNES, M.
700 1 $aBARBOSA, A. C. O.
700 1 $aSANTOS, T. A.
700 1 $aSILVA, A. G. S.
700 1 $aSANTOS, R. M. F.
700 1 $aAMORIM, E. P.
700 1 $aOLIVEIRA, S. A. S. de
700 1 $aJESUS, O. N. de
773 $tGenetics and Molecular Research$gv. 18, n.3, 2019.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Biblioteca |
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Classificação |
Cutter |
Registro |
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Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
04/09/2001 |
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Data da última atualização: |
10/12/2018 |
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Autoria: |
SANGOI, L.; ENDER, M.; GUIDOLIN, A. F.; ALMEIDA, M. L. de; KONFLANZ, V. A. |
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Afiliação: |
Luís Sangoi, Universidade do Estado de Santa Catarina - UDESC/Departamento de Fitotecnia; Márcio Ender, Universidade do Estado de Santa Catarina - UDESC/Departamento de Fitotecnia; Altamir Frederico Guidolin, Universidade do Estado de Santa Catarina - UDESC/Departamento de Fitotecnia; Milton Luiz de Almeida, Universidade Estadual de Santa Catarina - UDESC/Departamento de Fitotecnia. CNPq scholar; Valmor Antônio Konflanz, Universidade Estadual de Santa Catarina - UDESC/Departamento de Fitotecnia. CNPq scholar. |
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Título: |
Nitrogen fertilization impact on agronomic traits of maize hybrids released at different decades. |
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Ano de publicação: |
2001 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 36, n. 5, p. 757-64, maio 2001 |
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Idioma: |
Inglês |
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Notas: |
Título em português: Impacto da fertilização nitrogenada sobre características agronômicas de híbridos de milho lançados em diferentes décadas. |
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Conteúdo: |
Genetic selection of maize hybrids is often conducted using high N rates during the breeding cycle. This procedure may either lead to the release of genotypes that present nitrogen luxury consumption or require a stronger N input to accomplish their yield potential. This work was carried out to evaluate the effects of N rates on grain yield and N use efficiency of hybrids cultivated in different decades in Southern Brazil. The trial was performed in Lages, Santa Catarina State. A split plot design was used. Hybrids Ag 12, Ag 28, Ag 303 and Ag 9012, released during the 60's, 70's, 80's and 90's, respectively, were evaluated in the main plots. Nitrogen rates equivalent to 0, 50, 100 and 200 kg ha-1 were side-dressed in the split-plots when each hybrid had six fully expanded leaves. Modern-day hybrid Ag 9012 had higher grain yield than hybrids of earlier eras, regardless of N rates. Under high doses of N, the older hybrids Ag 12 and Ag 28 took up more N and presented higher values of shoot dry matter at flowering than Ag 9012. Nonetheless, they set less grains per ear which contributed to decreasetheir grain yield and N use efficiency. |
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Palavras-Chave: |
Hibridos; Rendimento de cultura. |
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Thesagro: |
Absorção; Zea Mays. |
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Thesaurus NAL: |
absorption; crop yield; hybrids. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/AI-SEDE/19122/1/pab0757.pdf
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Marc: |
LEADER 02037naa a2200265 a 4500
001 1103890
005 2018-12-10
008 2001 bl uuuu u00u1 u #d
100 1 $aSANGOI, L.
245 $aNitrogen fertilization impact on agronomic traits of maize hybrids released at different decades.
260 $c2001
500 $aTítulo em português: Impacto da fertilização nitrogenada sobre características agronômicas de híbridos de milho lançados em diferentes décadas.
520 $aGenetic selection of maize hybrids is often conducted using high N rates during the breeding cycle. This procedure may either lead to the release of genotypes that present nitrogen luxury consumption or require a stronger N input to accomplish their yield potential. This work was carried out to evaluate the effects of N rates on grain yield and N use efficiency of hybrids cultivated in different decades in Southern Brazil. The trial was performed in Lages, Santa Catarina State. A split plot design was used. Hybrids Ag 12, Ag 28, Ag 303 and Ag 9012, released during the 60's, 70's, 80's and 90's, respectively, were evaluated in the main plots. Nitrogen rates equivalent to 0, 50, 100 and 200 kg ha-1 were side-dressed in the split-plots when each hybrid had six fully expanded leaves. Modern-day hybrid Ag 9012 had higher grain yield than hybrids of earlier eras, regardless of N rates. Under high doses of N, the older hybrids Ag 12 and Ag 28 took up more N and presented higher values of shoot dry matter at flowering than Ag 9012. Nonetheless, they set less grains per ear which contributed to decreasetheir grain yield and N use efficiency.
650 $aabsorption
650 $acrop yield
650 $ahybrids
650 $aAbsorção
650 $aZea Mays
653 $aHibridos
653 $aRendimento de cultura
700 1 $aENDER, M.
700 1 $aGUIDOLIN, A. F.
700 1 $aALMEIDA, M. L. de
700 1 $aKONFLANZ, V. A.
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 36, n. 5, p. 757-64, maio 2001
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Embrapa Unidades Centrais (AI-SEDE) |
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