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 | Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
31/01/2013 |
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Data da última atualização: |
04/07/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOUZA, M. T. de; LIMA. M. I.; WERNECK, J. O. S.; DIAS, J. M. C. S. |
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Afiliação: |
MARLENE TEIXEIRA DE SOUZA, UnB; MARIA ISABEL LIMA, UnB; JOSEILDE OLIVEIRA SILVA WERNECK, CENARGEN; JOSE M. C. S. DIAS. |
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Título: |
Ultrastructural and molecular characterization of the Parasporal Crystal Proteins of Bacillus thuringiensis subsp. kurstaki S93 active against Spodoptera frugiperda. |
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Ano de publicação: |
1999 |
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Fonte/Imprenta: |
Biocell, v. 23, n. 1, p. 43-49, 1999. |
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Idioma: |
Inglês |
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Conteúdo: |
A Brazilian strain of Bacillus thuringiensis subsp. kurstaki (S93), which was previously shown to be more toxic to Spodoptera frugiperda than the commercially available strain HDI DIPEL, was characterized. The S93 isolate was shown to produce bypiramidal and cuboidal shape crystals. SDS-PAGE of sporulating cultures showed the presence of mainly two polypeptides of ca. 135- and 65-kDa, respectively. The presence of these polypeptides was in agreement with the type of crystals found. crylA- and cryIIA-type genes were also detected in the plasmidial DNA from this strains by using PCR. Furthermore, plasmidial profile of this strain has a very similar content to the HD1 DIPEL strain. These results hint that the Brazilian strain is very similar to the HD1 in several aspects. However, the reasons for the increased activity of the Brazilian isolate towards S. frugiperda remain unknown. |
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Palavras-Chave: |
Cry proteins. |
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Thesagro: |
Bacillus Thuringiensis; Spodoptera Frugiperda. |
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Thesaurus Nal: |
biological control. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01577naa a2200205 a 4500
001 1947172
005 2025-07-04
008 1999 bl uuuu u00u1 u #d
100 1 $aSOUZA, M. T. de
245 $aUltrastructural and molecular characterization of the Parasporal Crystal Proteins of Bacillus thuringiensis subsp. kurstaki S93 active against Spodoptera frugiperda.$h[electronic resource]
260 $c1999
520 $aA Brazilian strain of Bacillus thuringiensis subsp. kurstaki (S93), which was previously shown to be more toxic to Spodoptera frugiperda than the commercially available strain HDI DIPEL, was characterized. The S93 isolate was shown to produce bypiramidal and cuboidal shape crystals. SDS-PAGE of sporulating cultures showed the presence of mainly two polypeptides of ca. 135- and 65-kDa, respectively. The presence of these polypeptides was in agreement with the type of crystals found. crylA- and cryIIA-type genes were also detected in the plasmidial DNA from this strains by using PCR. Furthermore, plasmidial profile of this strain has a very similar content to the HD1 DIPEL strain. These results hint that the Brazilian strain is very similar to the HD1 in several aspects. However, the reasons for the increased activity of the Brazilian isolate towards S. frugiperda remain unknown.
650 $abiological control
650 $aBacillus Thuringiensis
650 $aSpodoptera Frugiperda
653 $aCry proteins
700 1 $aLIMA. M. I.
700 1 $aWERNECK, J. O. S.
700 1 $aDIAS, J. M. C. S
773 $tBiocell$gv. 23, n. 1, p. 43-49, 1999.
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| 2. |  | BRAZ, T. G. dos S.; JANK, L.; FONSECA, D. M. da; RAMÍREZ, N. E. M.; GUTIÉRREZ, M. M. A.; MARTUSCELLO, J. A. Análise dos componentes principais em híbridos de Panicum maximum. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 47., 2010, Salvador. Empreendedorismo e progresso científicos na zootecnia brasileira de vanguarda: anais. Salvador: SBZ, 2010. 1 CD-ROM. 3 p. R3708| Tipo: Artigo em Anais de Congresso |
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