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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
09/01/2013 |
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Data da última atualização: |
25/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA, E. J. de; OLIVEIRA, G. A. F.; COSTA, J. L.; OLIVEIRA, V. J. dos S. de; DANTAS, A. C. V. L.; DANTAS, J. L. L.; PADUA, J. G. |
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Afiliação: |
EDER JORGE DE OLIVEIRA, CNPMF; GILMARA ALVARENGA FACHARDO OLIVEIRA, UFRB; JULIANA LELES COSTA, UFRB; VÂNIA JESUS DOS SANTOS DE OLIVEIRA, UFRB; ANA CRISTINA VELLO LOYOLA DANTAS, UFRB; JORGE LUIZ LOYOLA DANTAS, CNPMF; JULIANO GOMES PADUA, CENARGEN. |
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Título: |
Genetic diversity and marker-assisted inbreeding in papaya. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Scientia Horticulturae, v. 147, p.20-28, 2012. |
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Idioma: |
Inglês |
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Conteúdo: |
Conventional methods for the selection of papaya inbred lines are time-consuming. Procedures involving the use of molecular markers can reduce this time considerably, by indirect selection of homozygous plants. The objective of this study was to use microsatellite markers in marker-assisted selection (MAS) to develop new papaya inbred lines, and categorize the inbred lines into useful groups based on the molecular profiles and agronomic traits. Thirty-six lines from F4 (Calimosa × Common papaya) and S3 derivate from open-population accessions of germplasm, and 6 controls were used for the molecular analysis with 27 microsatellite primers. Twelve polymorphic and 15 monomorphic microsatellite primers were identified, allowing the identification of 59 alleles, with an average of 2.19 alleles per primer. The observed heterozygosity values (Ho) were low for both the markers (0.00-0.24) and the individual lines (0.00-0.19). The inbreeding coefficient (f) ranged from 0.80 to 1.00. Eleven papaya inbred lines and checks (landrace, cultivars, and other pure lines) showed complete inbreeding (f = 1). A broad genetic variability for all agronomic traits analyzed, especially for productivity (10.34-86.39 t ha-1), was observed and was confirmed using agronomic and molecular data simultaneously to group the new papaya inbred lines and checks. Hierarchical and silhouette-width method using Gower's dissimilarity revealed 9 major clusters. Despite the fact that self-pollination and selection lead to reduction of genetic variability, papaya inbred lines are proved to be genetically diverse representing a large proportion of the genetic variation occurring in ?Solo? and ?Formosa? groups. This seems to be promising for success in further developments of new hybrids in breeding programs. MenosConventional methods for the selection of papaya inbred lines are time-consuming. Procedures involving the use of molecular markers can reduce this time considerably, by indirect selection of homozygous plants. The objective of this study was to use microsatellite markers in marker-assisted selection (MAS) to develop new papaya inbred lines, and categorize the inbred lines into useful groups based on the molecular profiles and agronomic traits. Thirty-six lines from F4 (Calimosa × Common papaya) and S3 derivate from open-population accessions of germplasm, and 6 controls were used for the molecular analysis with 27 microsatellite primers. Twelve polymorphic and 15 monomorphic microsatellite primers were identified, allowing the identification of 59 alleles, with an average of 2.19 alleles per primer. The observed heterozygosity values (Ho) were low for both the markers (0.00-0.24) and the individual lines (0.00-0.19). The inbreeding coefficient (f) ranged from 0.80 to 1.00. Eleven papaya inbred lines and checks (landrace, cultivars, and other pure lines) showed complete inbreeding (f = 1). A broad genetic variability for all agronomic traits analyzed, especially for productivity (10.34-86.39 t ha-1), was observed and was confirmed using agronomic and molecular data simultaneously to group the new papaya inbred lines and checks. Hierarchical and silhouette-width method using Gower's dissimilarity revealed 9 major clusters. Despite the fact that self-pollination and selection ... Mostrar Tudo |
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Palavras-Chave: |
Genetic diversity; Papaya. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02415naa a2200217 a 4500
001 1944564
005 2023-05-25
008 2012 bl uuuu u00u1 u #d
100 1 $aOLIVEIRA, E. J. de
245 $aGenetic diversity and marker-assisted inbreeding in papaya.$h[electronic resource]
260 $c2012
520 $aConventional methods for the selection of papaya inbred lines are time-consuming. Procedures involving the use of molecular markers can reduce this time considerably, by indirect selection of homozygous plants. The objective of this study was to use microsatellite markers in marker-assisted selection (MAS) to develop new papaya inbred lines, and categorize the inbred lines into useful groups based on the molecular profiles and agronomic traits. Thirty-six lines from F4 (Calimosa × Common papaya) and S3 derivate from open-population accessions of germplasm, and 6 controls were used for the molecular analysis with 27 microsatellite primers. Twelve polymorphic and 15 monomorphic microsatellite primers were identified, allowing the identification of 59 alleles, with an average of 2.19 alleles per primer. The observed heterozygosity values (Ho) were low for both the markers (0.00-0.24) and the individual lines (0.00-0.19). The inbreeding coefficient (f) ranged from 0.80 to 1.00. Eleven papaya inbred lines and checks (landrace, cultivars, and other pure lines) showed complete inbreeding (f = 1). A broad genetic variability for all agronomic traits analyzed, especially for productivity (10.34-86.39 t ha-1), was observed and was confirmed using agronomic and molecular data simultaneously to group the new papaya inbred lines and checks. Hierarchical and silhouette-width method using Gower's dissimilarity revealed 9 major clusters. Despite the fact that self-pollination and selection lead to reduction of genetic variability, papaya inbred lines are proved to be genetically diverse representing a large proportion of the genetic variation occurring in ?Solo? and ?Formosa? groups. This seems to be promising for success in further developments of new hybrids in breeding programs.
653 $aGenetic diversity
653 $aPapaya
700 1 $aOLIVEIRA, G. A. F.
700 1 $aCOSTA, J. L.
700 1 $aOLIVEIRA, V. J. dos S. de
700 1 $aDANTAS, A. C. V. L.
700 1 $aDANTAS, J. L. L.
700 1 $aPADUA, J. G.
773 $tScientia Horticulturae$gv. 147, p.20-28, 2012.
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| 1. |  | KANEKO, N.; KAWAGUCHI, T.; TOYOTA, A.; NISHIZAWA, T.; NAGAOSA, K.; IWASHIMA, N.; OHTA, H.; KATO, K.; MASUNAGA, T.; MURAKAMI, M.; HIURA, T. Link earthworm activity to plant growth by cast analysis in a cool temperate forest in Japan. In: INTERNATIONAL COLLOQUIUM ON SOIL ZOOLOGY, 15; INTERNATIONAL COLLOQUIUM ON APTERYGOTA, 12., 2008, Curitiba. Biodiversity, conservation and sustainabele management of soil animal: abstracts. Colombo: Embrapa Florestas. Editors: George Gardner Brown; Klaus Dieter Sautter; Renato Marques; Amarildo Pasini. 1 CD-ROM.| Biblioteca(s): Embrapa Florestas. |
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