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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
03/10/2011 |
Data da última atualização: |
05/02/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
BRITO, M. A. V. P. e; SOMKUTI, G. A.; RENYE JUNIOR, J. A. |
Afiliação: |
MARIA APARECIDA V PAIVA E BRITO, CNPGL; G. A. SOMKUTI, Eastern Regional Research Center, U. S. Departmento of Agriculture; J. A. RENYE JUNIOR, Eastern Regional Research Center, U. S. Departmento of Agriculture. |
Título: |
Production of antilisterial bacteriocins by staphylococci isolated from bovine milk. |
Ano de publicação: |
2011 |
Fonte/Imprenta: |
Journal of Dairy Science, v. 94, p. 1194-1200, 2011. |
DOI: |
https://doi.org/10.3168/jds.2010-3849 |
Idioma: |
Inglês |
Conteúdo: |
A collection of 111 staphylococcal isolates recovered from healthy cows in 41 dairy herds in Brazil was surveyed for the production of bacteriocins. The group included 94 coagulase-positive and 17 coagulase-negative strains of staphylococci. All cultures were grown in tryptic soy broth for 18h at 37°C, and cell-free supernatants were tested for antimicrobial activity against several target organisms by using the agar diffusion method. Filtrates of 57 staphylococci showed strong activity against Listeria monocytogenes Scott A, and 52 isolates also inhibited the growth of Stapylococcus aureus Newbould 305, a major causative agent of bovine mastitis in the United States. The plasmid profiles of staphylococci invariably included an 8-kb plasmid. Staphylococcal isolates were tested for the production of aureocins A70 and A53, 2 bacteriocins of coagulase-positive staphylococci known to be associated with 8-kb and 10.2-kb plasmids, respectively. The presence of the A70 or A53 bacteriocin gene was checked by PCR techniques using primers based on nucleotide sequences flanking the structural gene of each bacteriocin. Agarose gel analysis of amplified PCR products of plasmid templates from all 58 isolates showed only a 525-bp fragment corresponding to the structural gene of the bacteriocin aureocin A70. The results indicated that the apparently widespread association of A70-producing staphylococci with healthy cows in Brazil may be beneficial in controlling undesirable bacteria in dairy herds. MenosA collection of 111 staphylococcal isolates recovered from healthy cows in 41 dairy herds in Brazil was surveyed for the production of bacteriocins. The group included 94 coagulase-positive and 17 coagulase-negative strains of staphylococci. All cultures were grown in tryptic soy broth for 18h at 37°C, and cell-free supernatants were tested for antimicrobial activity against several target organisms by using the agar diffusion method. Filtrates of 57 staphylococci showed strong activity against Listeria monocytogenes Scott A, and 52 isolates also inhibited the growth of Stapylococcus aureus Newbould 305, a major causative agent of bovine mastitis in the United States. The plasmid profiles of staphylococci invariably included an 8-kb plasmid. Staphylococcal isolates were tested for the production of aureocins A70 and A53, 2 bacteriocins of coagulase-positive staphylococci known to be associated with 8-kb and 10.2-kb plasmids, respectively. The presence of the A70 or A53 bacteriocin gene was checked by PCR techniques using primers based on nucleotide sequences flanking the structural gene of each bacteriocin. Agarose gel analysis of amplified PCR products of plasmid templates from all 58 isolates showed only a 525-bp fragment corresponding to the structural gene of the bacteriocin aureocin A70. The results indicated that the apparently widespread association of A70-producing staphylococci with healthy cows in Brazil may be beneficial in controlling undesirable bacteria in dair... Mostrar Tudo |
Palavras-Chave: |
Antilisterial activity; Bacteriocin; Staphylococci from milk. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02131naa a2200193 a 4500 001 1902017 005 2024-02-05 008 2011 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.3168/jds.2010-3849$2DOI 100 1 $aBRITO, M. A. V. P. e 245 $aProduction of antilisterial bacteriocins by staphylococci isolated from bovine milk.$h[electronic resource] 260 $c2011 520 $aA collection of 111 staphylococcal isolates recovered from healthy cows in 41 dairy herds in Brazil was surveyed for the production of bacteriocins. The group included 94 coagulase-positive and 17 coagulase-negative strains of staphylococci. All cultures were grown in tryptic soy broth for 18h at 37°C, and cell-free supernatants were tested for antimicrobial activity against several target organisms by using the agar diffusion method. Filtrates of 57 staphylococci showed strong activity against Listeria monocytogenes Scott A, and 52 isolates also inhibited the growth of Stapylococcus aureus Newbould 305, a major causative agent of bovine mastitis in the United States. The plasmid profiles of staphylococci invariably included an 8-kb plasmid. Staphylococcal isolates were tested for the production of aureocins A70 and A53, 2 bacteriocins of coagulase-positive staphylococci known to be associated with 8-kb and 10.2-kb plasmids, respectively. The presence of the A70 or A53 bacteriocin gene was checked by PCR techniques using primers based on nucleotide sequences flanking the structural gene of each bacteriocin. Agarose gel analysis of amplified PCR products of plasmid templates from all 58 isolates showed only a 525-bp fragment corresponding to the structural gene of the bacteriocin aureocin A70. The results indicated that the apparently widespread association of A70-producing staphylococci with healthy cows in Brazil may be beneficial in controlling undesirable bacteria in dairy herds. 653 $aAntilisterial activity 653 $aBacteriocin 653 $aStaphylococci from milk 700 1 $aSOMKUTI, G. A. 700 1 $aRENYE JUNIOR, J. A. 773 $tJournal of Dairy Science$gv. 94, p. 1194-1200, 2011.
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Embrapa Gado de Leite (CNPGL) |
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Biblioteca(s): |
Embrapa Pecuária Sul. |
Data corrente: |
17/11/2017 |
Data da última atualização: |
18/12/2017 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
MELLO, M. B. de; SILVA, G. M. da. |
Afiliação: |
Marlon Bandeira de Mello, UNIJUÍ; GUSTAVO MARTINS DA SILVA, CPPSUL. |
Título: |
Análise e diagnóstico da produção de sementes de azevém (Lolium multiflorum Lam.). |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
In: SIMPÓSIO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA PECUÁRIA SUL, 7., 2017, Bagé. Resumos... Bagé: Embrapa Pecuária Sul, 2017. |
Páginas: |
p. 12. |
Idioma: |
Português |
Notas: |
Claudia Cristina Gulias Gomes, editora técnica. |
Conteúdo: |
O objetivo do trabalho foi avaliar o processo de produção de sementes de azevém na região norte do Rio Grande do Sul, identificando e buscando soluções para os principais fatores limitantes. |
Thesagro: |
Azevém; Gramínea forrageira; Lolium multiflorum; Semente. |
Categoria do assunto: |
-- |
URL: |
https://www.alice.cnptia.embrapa.br/alice/bitstream/doc/1080056/1/AnaliseediagnosticodaproducaodesementesdeazevemLoliummultiflorumLam..pdf
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Marc: |
LEADER 00880nam a2200193 a 4500 001 2080056 005 2017-12-18 008 2017 bl uuuu u00u1 u #d 100 1 $aMELLO, M. B. de 245 $aAnálise e diagnóstico da produção de sementes de azevém (Lolium multiflorum Lam.).$h[electronic resource] 260 $aIn: SIMPÓSIO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA PECUÁRIA SUL, 7., 2017, Bagé. Resumos... Bagé: Embrapa Pecuária Sul$c2017 300 $ap. 12. 500 $aClaudia Cristina Gulias Gomes, editora técnica. 520 $aO objetivo do trabalho foi avaliar o processo de produção de sementes de azevém na região norte do Rio Grande do Sul, identificando e buscando soluções para os principais fatores limitantes. 650 $aAzevém 650 $aGramínea forrageira 650 $aLolium multiflorum 650 $aSemente 700 1 $aSILVA, G. M. da
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