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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
27/04/2011 |
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Data da última atualização: |
29/04/2011 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
MARTINS, P. K.; MAFRA, V. S.; KISHI, L. T.; FREITAS-ASTUA, J. |
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Afiliação: |
Polyanna Kelly Martins, APTA; Valéria S. Mafra, APTA; Luciano T. Kishi, APTA; JULIANA DE FREITAS ASTUA, CNPMF. |
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Título: |
Phylogenetic and comparative gene expression analysis of citrus WRKY transcription factor family. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3, 2011, Ilhéus. Resumos. [S. l.]: Sociedade Brasileira de Genética, 2011. 1 CD-ROM. |
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Idioma: |
Inglês |
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Notas: |
pdf 35130 |
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Conteúdo: |
Plants have a variety of active defense mechanisms to protect themselves from pathogen infection. Plant defense response results from the transcriptional activation of a large number of genes upon pathogen infection or treatment with pathogen elicitors. Among them, WRKY proteins are considered important transcriptional regulators in defense signaling, including the activation of SAR (systemic acquired resistance). Several genes regulating plant defense, such as NPR1 and PR (Pathogenesis-related proteins), have W-box elements in their promoters that are specifically recognized by WRKY proteins also required for induction or expression. The defining feature of WRKY transcription factors is their DNA binding domain. The WRKY domain is about 60 residues in length that contains the WRKY signature and also an atypical zinc-finger structure at the C-terminus (Cx4?5Cx22?23HxH or Cx7Cx23HxC). The aim of this work was to identify all potential WRKY transcription factors in citrus, retrieved from the CitEST (Citrus ESTs) database and to construct a phylogenetic tree with orthologs from Arabidopsis thaliana and Oryza sativa. To find ESTs coding for WRKY proteins in citrus we performed a tBLASTn search on the CitEST database. The WRKY domain sequences of seven Arabidopsis WRKY family members each representing one of the WRKY subgroups were used as query sequences. Phylogenetic tree were constructed by the neighbor-joining (NJ) method in MEGA 4. NJ analysis was performed with the Pairwise Deletion option and the Poisson correction. For statistical reliability, bootstrap analysis was conducted with 1,000 replicates to assess statistical support for each node. The primary search resulted in 71 non-redundant hits, of which 9 were removed as they did not contain the conserved WRKY domain signature. The remaining 62 sequences were used to construct the phylogenetic tree; this analysis identified 19 unigenes for the group 1; 6 unigenes for the subgroup 2a, 3 unigenes for the subgroup 2b, 12 unigenes for the subgroup 2c, 13 unigenes for the group 2d, 9 unigenes for subgroup 2e and 7 unigenes for the group 3. To complete the phylogenetic analysis, WRKY sequences from the draft sequencing of the genomes of Citrus clementina and C. sinensis will be included. We are particularly interested in WRKY proteins involved in biotic stress, so the next step will be to evaluate the transcriptional profile of some WRKY genes under different biotic stress conditions. This study will be crucial to select candidates for citrus genetic transformation. MenosPlants have a variety of active defense mechanisms to protect themselves from pathogen infection. Plant defense response results from the transcriptional activation of a large number of genes upon pathogen infection or treatment with pathogen elicitors. Among them, WRKY proteins are considered important transcriptional regulators in defense signaling, including the activation of SAR (systemic acquired resistance). Several genes regulating plant defense, such as NPR1 and PR (Pathogenesis-related proteins), have W-box elements in their promoters that are specifically recognized by WRKY proteins also required for induction or expression. The defining feature of WRKY transcription factors is their DNA binding domain. The WRKY domain is about 60 residues in length that contains the WRKY signature and also an atypical zinc-finger structure at the C-terminus (Cx4?5Cx22?23HxH or Cx7Cx23HxC). The aim of this work was to identify all potential WRKY transcription factors in citrus, retrieved from the CitEST (Citrus ESTs) database and to construct a phylogenetic tree with orthologs from Arabidopsis thaliana and Oryza sativa. To find ESTs coding for WRKY proteins in citrus we performed a tBLASTn search on the CitEST database. The WRKY domain sequences of seven Arabidopsis WRKY family members each representing one of the WRKY subgroups were used as query sequences. Phylogenetic tree were constructed by the neighbor-joining (NJ) method in MEGA 4. NJ analysis was performed with the Pairwise... Mostrar Tudo |
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Palavras-Chave: |
CitEST; SAR; W-box. |
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Thesaurus Nal: |
Citrus; transcription factors. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 03255nam a2200217 a 4500
001 1886952
005 2011-04-29
008 2011 bl uuuu u00u1 u #d
100 1 $aMARTINS, P. K.
245 $aPhylogenetic and comparative gene expression analysis of citrus WRKY transcription factor family.
260 $aIn: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3, 2011, Ilhéus. Resumos. [S. l.]: Sociedade Brasileira de Genética, 2011. 1 CD-ROM.$c2011
500 $apdf 35130
520 $aPlants have a variety of active defense mechanisms to protect themselves from pathogen infection. Plant defense response results from the transcriptional activation of a large number of genes upon pathogen infection or treatment with pathogen elicitors. Among them, WRKY proteins are considered important transcriptional regulators in defense signaling, including the activation of SAR (systemic acquired resistance). Several genes regulating plant defense, such as NPR1 and PR (Pathogenesis-related proteins), have W-box elements in their promoters that are specifically recognized by WRKY proteins also required for induction or expression. The defining feature of WRKY transcription factors is their DNA binding domain. The WRKY domain is about 60 residues in length that contains the WRKY signature and also an atypical zinc-finger structure at the C-terminus (Cx4?5Cx22?23HxH or Cx7Cx23HxC). The aim of this work was to identify all potential WRKY transcription factors in citrus, retrieved from the CitEST (Citrus ESTs) database and to construct a phylogenetic tree with orthologs from Arabidopsis thaliana and Oryza sativa. To find ESTs coding for WRKY proteins in citrus we performed a tBLASTn search on the CitEST database. The WRKY domain sequences of seven Arabidopsis WRKY family members each representing one of the WRKY subgroups were used as query sequences. Phylogenetic tree were constructed by the neighbor-joining (NJ) method in MEGA 4. NJ analysis was performed with the Pairwise Deletion option and the Poisson correction. For statistical reliability, bootstrap analysis was conducted with 1,000 replicates to assess statistical support for each node. The primary search resulted in 71 non-redundant hits, of which 9 were removed as they did not contain the conserved WRKY domain signature. The remaining 62 sequences were used to construct the phylogenetic tree; this analysis identified 19 unigenes for the group 1; 6 unigenes for the subgroup 2a, 3 unigenes for the subgroup 2b, 12 unigenes for the subgroup 2c, 13 unigenes for the group 2d, 9 unigenes for subgroup 2e and 7 unigenes for the group 3. To complete the phylogenetic analysis, WRKY sequences from the draft sequencing of the genomes of Citrus clementina and C. sinensis will be included. We are particularly interested in WRKY proteins involved in biotic stress, so the next step will be to evaluate the transcriptional profile of some WRKY genes under different biotic stress conditions. This study will be crucial to select candidates for citrus genetic transformation.
650 $aCitrus
650 $atranscription factors
653 $aCitEST
653 $aSAR
653 $aW-box
700 1 $aMAFRA, V. S.
700 1 $aKISHI, L. T.
700 1 $aFREITAS-ASTUA, J.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
09/05/2022 |
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Data da última atualização: |
01/06/2022 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
MARCHESINI, P.; TEIXEIRA, A. L. C.; CARDOSO, S. J.; PRATA, M. C. de A.; NASCIMENTO, R. M.; KLAFKE, G.; COSTA-JUNIOR, L. M.; MATURANO, R.; LOPES, W. D. Z.; BITTENCOURT, V. R. E. P.; MONTEIRO, C. M. |
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Afiliação: |
PAULA BARROSO CRUZ MARCHESINI, Universidade Federal de Goiás; ANA LÚCIA COUTINHO TEIXEIRA, Universidade Federal de Goiás; SIMONE JAQUELINE CARDOSO, Universidade Federal de Juiz de Fora; MARCIA CRISTINA DE AZEVEDO PRATA, CNPGL; RAFAEL MOREIRA DO NASCIMENTO, Universidade Federal Rural do Rio de Janeiro; GUILHERME KLAFKE, Instituto de Pesquisas Veterinárias Desidério Finamor; LÍVIO MARTINS COSTA JÚNIOR, Universidade Federal do Maranhão; RALPH MATURANO, Universidade Federal de Juiz de Fora; WELBER DANIEL ZANETTI LOPES, Universidade Federal de Goiás; VÂNIA RITA ELIAS PINHEIRO BITTENCOURT, Universidade Federal Rural do Rio de Janeiro; CAIO MONTEIRO, Universidade Federal de Goiás. |
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Título: |
Atividade carrapaticida do alfa-bisabolol sobre populações de Rhipicephalus microplus (acari: ixodidae) com diferentes perfis de resistência. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
In: SIMPÓSIO BRASILEIRO DE ACAROLOGIA, 7., 2021. Acarologia em tempos de conectividade: [resumos]. México: Sociedad Latinoamericana de Acarologia, 2022. |
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Idioma: |
Português |
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Notas: |
Evento online. |
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Conteúdo: |
O presente estudo teve como objetivo investigar a atividade acaricida do alfa-bisabolol sobre populações de Rhipicephalus microplus, com diferentes perfis de resistência. |
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Palavras-Chave: |
Resistência cruzada; Sesquiterpeno. |
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Thesagro: |
Acaricida; Bovino; Carrapaticida; Carrapato. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1142772/1/Atividade-carrapaticida.pdf
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Marc: |
LEADER 01232nam a2200313 a 4500
001 2142772
005 2022-06-01
008 2022 bl uuuu u00u1 u #d
100 1 $aMARCHESINI, P.
245 $aAtividade carrapaticida do alfa-bisabolol sobre populações de Rhipicephalus microplus (acari$bixodidae) com diferentes perfis de resistência.$h[electronic resource]
260 $aIn: SIMPÓSIO BRASILEIRO DE ACAROLOGIA, 7., 2021. Acarologia em tempos de conectividade: [resumos]. México: Sociedad Latinoamericana de Acarologia$c2022
500 $aEvento online.
520 $aO presente estudo teve como objetivo investigar a atividade acaricida do alfa-bisabolol sobre populações de Rhipicephalus microplus, com diferentes perfis de resistência.
650 $aAcaricida
650 $aBovino
650 $aCarrapaticida
650 $aCarrapato
653 $aResistência cruzada
653 $aSesquiterpeno
700 1 $aTEIXEIRA, A. L. C.
700 1 $aCARDOSO, S. J.
700 1 $aPRATA, M. C. de A.
700 1 $aNASCIMENTO, R. M.
700 1 $aKLAFKE, G.
700 1 $aCOSTA-JUNIOR, L. M.
700 1 $aMATURANO, R.
700 1 $aLOPES, W. D. Z.
700 1 $aBITTENCOURT, V. R. E. P.
700 1 $aMONTEIRO, C. M.
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