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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
28/09/2010 |
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Data da última atualização: |
30/06/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SARAIVA, N. Z.; OLIVEIRA, C. S.; TETZNER, T. A. D.; SOUZA, M. M.; LIMA, M. R. de; NICIURA, S. C. M.; GARCIA, J. M. |
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Afiliação: |
N. Z. SARAIVA, UNESP/JABOTICABAL; C. S. OLIVEIRA, UNESP/JABOTICABAL; T. A. D. TETZNER, UNESP/JABOTICABAL; M. M. SOUZA, UNESP/JABOTICABAL; M. R. DE LIMA, UNESP/JABOTICABAL; SIMONE CRISTINA MEO NICIURA, CPPSE; J. M. GARCIA, UNESP/JABOTICABAL. |
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Título: |
Bovine cytoplasts prepared by demecolcine-induced enucleation of activated oocytes. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
In: ANNUAL CONFERENCE OT THE INTERNATIONAL EMBRYO TRANSFER SOCIETY, 36., 2010, Cordoba. Proceedings... Córdoba: ITES, 2010; Reproduction, Fertility and development, v. 22, n. 1, 2010. |
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Páginas: |
p. 197 |
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Idioma: |
Português |
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Conteúdo: |
One of the most critical steps of the standard NT procedure is the removal of oocyte chromosomes for the production of enucleated cytoplasts. This process involves ultraviolet (UV) irradiation, which causes damage to the membrane and intracellular components of bovine oocytes. The objective of the present study was to evaluate the use of demecolcine, a microtubule depolymerizing agent, for chemical-induced enucleation of activated bovine oocytes. In the first experiment, oocytes obtained from cow ovaries collected in a slaughterhouse were IVM in TCM-199 medium supplemented with 10% FCS, FSH, hCG, estradiol, pyruvate, and amikacin for 26 h; then, they were artificially activated with 5 ?M ionomycin for 5 min and 10 ?g mL-1 cycloheximide for 4 h. The following groups were established: control, C (no activation and no demecolcine); activated, A (exposure only to activating agents); and demecolcine-exposed oocytes, DEME (activation for 4 h and 0.05 ?g mL-1 demecolcine for 2 to 4 h after 0, 0.5, 1.0, 1.5, and 2.0 h of activation). The treatment DEME comprised the groups G1 (demecolcine from 0 to 2 h of activation; DEME 0-2 h); G2 (DEME 0-4 h); G3 (DEME 0.5-2.5 h); G4 (DEME 0.5-4 h); G5 (DEME 1-3 h); G6 (DEME 1-4 h); G7 (DEME 1.5-3.5 h); G8 (DEME 1.5-4 h); and G9 (DEME 2-4h). The oocytes were stained with 10 ?g mL-1 Hoechst 33342 for 15 min and enucleation rates (EN) were evaluated under epifluorescence microscope (330-385 nm).After EN evaluation, a second experiment was performed to verify the efficiency of demecolcine-induced enucleation in group G9, which showed the best result in the first experiment. After demecolcine treatment, the oocytes were incubated in HEPES-buffered SOF with 10% FCS and cytochalasin B for removal of the 2PB and minimal adjacent cytoplast under an inverted optical microscope. Traditional enucleation was performed on the control group without exposure of the oocytes to demecolcine. The same conditions were employed except that UV light was used to confirm enucleation. Samples of cytoplasts were stained with Hoechst for 15 min and analyzed for enucleation efficiency. Five and 3 replicates were evaluated, respectively, in experiments 1 and 2, and the results were analyzed by chi-square test in the statistical software Minitab®, release 14.1 (Minitab Inc., State College, PA, USA). A level of 5% significance was used. Regarding enucleation rates, all treated groups were significantly different compared with the C (0/114) and A (0/130) groups. Considering the DEME groups, G8 (46/109; 42.2%) and G9 (61/113; 54.0%) presented superior rates (P < 0.05) to all other groups (26.8 to 36.3%), but they were similar despite the great tendency (P = 0.07) to difference. We observed high efficiency (P < 0.05) of demecolcine-induced enucleation (90/110; 81.8%) compared with a traditional technique (61/95; 64.2%). In conclusion, the present study shows that demecolcine-induced enucleation of activated oocytes enables good rates of enucleation and has greater efficiency than the traditional technique as well as avoiding UV irradiation of the cytoplast. MenosOne of the most critical steps of the standard NT procedure is the removal of oocyte chromosomes for the production of enucleated cytoplasts. This process involves ultraviolet (UV) irradiation, which causes damage to the membrane and intracellular components of bovine oocytes. The objective of the present study was to evaluate the use of demecolcine, a microtubule depolymerizing agent, for chemical-induced enucleation of activated bovine oocytes. In the first experiment, oocytes obtained from cow ovaries collected in a slaughterhouse were IVM in TCM-199 medium supplemented with 10% FCS, FSH, hCG, estradiol, pyruvate, and amikacin for 26 h; then, they were artificially activated with 5 ?M ionomycin for 5 min and 10 ?g mL-1 cycloheximide for 4 h. The following groups were established: control, C (no activation and no demecolcine); activated, A (exposure only to activating agents); and demecolcine-exposed oocytes, DEME (activation for 4 h and 0.05 ?g mL-1 demecolcine for 2 to 4 h after 0, 0.5, 1.0, 1.5, and 2.0 h of activation). The treatment DEME comprised the groups G1 (demecolcine from 0 to 2 h of activation; DEME 0-2 h); G2 (DEME 0-4 h); G3 (DEME 0.5-2.5 h); G4 (DEME 0.5-4 h); G5 (DEME 1-3 h); G6 (DEME 1-4 h); G7 (DEME 1.5-3.5 h); G8 (DEME 1.5-4 h); and G9 (DEME 2-4h). The oocytes were stained with 10 ?g mL-1 Hoechst 33342 for 15 min and enucleation rates (EN) were evaluated under epifluorescence microscope (330-385 nm).After EN evaluation, a second experiment was performed... Mostrar Tudo |
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Palavras-Chave: |
Bovine; Cytoplasts. |
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Thesaurus Nal: |
oocytes. |
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Categoria do assunto: |
W Química e Física |
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Marc: |
LEADER 03900nam a2200229 a 4500
001 1863081
005 2023-06-30
008 2010 bl uuuu u00u1 u #d
100 1 $aSARAIVA, N. Z.
245 $aBovine cytoplasts prepared by demecolcine-induced enucleation of activated oocytes.$h[electronic resource]
260 $aIn: ANNUAL CONFERENCE OT THE INTERNATIONAL EMBRYO TRANSFER SOCIETY, 36., 2010, Cordoba. Proceedings... Córdoba: ITES, 2010; Reproduction, Fertility and development, v. 22, n. 1, 2010.$c2010
300 $ap. 197
520 $aOne of the most critical steps of the standard NT procedure is the removal of oocyte chromosomes for the production of enucleated cytoplasts. This process involves ultraviolet (UV) irradiation, which causes damage to the membrane and intracellular components of bovine oocytes. The objective of the present study was to evaluate the use of demecolcine, a microtubule depolymerizing agent, for chemical-induced enucleation of activated bovine oocytes. In the first experiment, oocytes obtained from cow ovaries collected in a slaughterhouse were IVM in TCM-199 medium supplemented with 10% FCS, FSH, hCG, estradiol, pyruvate, and amikacin for 26 h; then, they were artificially activated with 5 ?M ionomycin for 5 min and 10 ?g mL-1 cycloheximide for 4 h. The following groups were established: control, C (no activation and no demecolcine); activated, A (exposure only to activating agents); and demecolcine-exposed oocytes, DEME (activation for 4 h and 0.05 ?g mL-1 demecolcine for 2 to 4 h after 0, 0.5, 1.0, 1.5, and 2.0 h of activation). The treatment DEME comprised the groups G1 (demecolcine from 0 to 2 h of activation; DEME 0-2 h); G2 (DEME 0-4 h); G3 (DEME 0.5-2.5 h); G4 (DEME 0.5-4 h); G5 (DEME 1-3 h); G6 (DEME 1-4 h); G7 (DEME 1.5-3.5 h); G8 (DEME 1.5-4 h); and G9 (DEME 2-4h). The oocytes were stained with 10 ?g mL-1 Hoechst 33342 for 15 min and enucleation rates (EN) were evaluated under epifluorescence microscope (330-385 nm).After EN evaluation, a second experiment was performed to verify the efficiency of demecolcine-induced enucleation in group G9, which showed the best result in the first experiment. After demecolcine treatment, the oocytes were incubated in HEPES-buffered SOF with 10% FCS and cytochalasin B for removal of the 2PB and minimal adjacent cytoplast under an inverted optical microscope. Traditional enucleation was performed on the control group without exposure of the oocytes to demecolcine. The same conditions were employed except that UV light was used to confirm enucleation. Samples of cytoplasts were stained with Hoechst for 15 min and analyzed for enucleation efficiency. Five and 3 replicates were evaluated, respectively, in experiments 1 and 2, and the results were analyzed by chi-square test in the statistical software Minitab®, release 14.1 (Minitab Inc., State College, PA, USA). A level of 5% significance was used. Regarding enucleation rates, all treated groups were significantly different compared with the C (0/114) and A (0/130) groups. Considering the DEME groups, G8 (46/109; 42.2%) and G9 (61/113; 54.0%) presented superior rates (P < 0.05) to all other groups (26.8 to 36.3%), but they were similar despite the great tendency (P = 0.07) to difference. We observed high efficiency (P < 0.05) of demecolcine-induced enucleation (90/110; 81.8%) compared with a traditional technique (61/95; 64.2%). In conclusion, the present study shows that demecolcine-induced enucleation of activated oocytes enables good rates of enucleation and has greater efficiency than the traditional technique as well as avoiding UV irradiation of the cytoplast.
650 $aoocytes
653 $aBovine
653 $aCytoplasts
700 1 $aOLIVEIRA, C. S.
700 1 $aTETZNER, T. A. D.
700 1 $aSOUZA, M. M.
700 1 $aLIMA, M. R. de
700 1 $aNICIURA, S. C. M.
700 1 $aGARCIA, J. M.
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| Registros recuperados : 388 | |
| 321. |  | QUINTINO, A. da C.; ABREU, J. G.; ALMEIDA, R. G. de; MACEDO, M. C. M.; CABRAL, L. DA S.; GALATI, R. L. Valor nutritivo de silagem de capim-piatã em monocultivo e em consórcio com sorgo de corte e pastejo. Ciência Animal Brasileira, Goiânia, v. 17, n. 2, p. 185-191, abr./jun. 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 322. | | SANTOS, V. A. C. dos; QUINTINO, A. da C.; ALMEIDA, R. G. de; ABREU, J. G. de; MACEDO, M. C. M.; BUNGENSTAB, D. J. Valor nutritivo da silagem de capim-piatã com e sem adição de farelo de arroz em diferentes idades de corte da forrageira, no outono-inverno¹. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 48., 2011, Belém, PA. O desenvolvimento da produção animal e a responsabilidade frente a novos desafios: anais. Belém, PA: SBZ, 2011. 1 CD-ROM. SBZ 2011. 1-3 1 CD-ROM SBZ 2011| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 323. | | VOLPI, D.; ALVES, F. V.; SARAIVA, E. F.; VIGAS, V. P.; ARGUELHO, A. da S.; ZOPOLLATTO, M.; ALMEIDA, R. G. de. Validação da bioacústica como alternativa à avaliação visual para monitoramento do comportamento ingestivo de bovinos em pastagens. Campo Grande, MS: Embrapa Gado de Corte, 2018 32 p. (Embrapa Gado de Corte. Boletim de Pesquisa e desenvolvimento, 40).| Tipo: Boletim de Pesquisa e Desenvolvimento |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 324. | | SERRA, A. P.; MARCHETTI, M. E.; DUPAS, E.; ENSINAS, S. C.; LOURENTE, E. R. P.; SILVA, E. F. da; ALMEIDA, R. G. de; CARDUCCI, C. E.; ALOVISI, A. M. T. Best Management Practices (BMPs) for Nitrogen Fertilizer in Forage Grasses. In: EDVAN, R. L. New Perspectives in Forage Crops. London, United Kingdom: IntechOpen, 2018 P. 243| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 325. | | SILVEIRA, M. C. T. da; SANTOS, F. C. dos; ALBUQUERQUE FILHO, M. R. de; ALMEIDA, R. G. de; ALVES, F. V.; VILELA, L.; BARBOSA, T. A.; PINTO, C. F.; FEIJO, G. L. D.; SIMEÃO, R. M. Beef production with low carbon emission in Tropical pastures: a case study for the validation of guidelines. In: WORLD CONGRESS ON INTEGRATED CROP-LIVESTOCK-FORESTRY SYSTEMS, 2., 2021. Proceedings reference... Brasília, DF: Embrapa, 2021. p. 128-133. WCCLF. Evento online.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo; Embrapa Pecuária Sul. |
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| 326. | | SILVEIRA, M. C. T. da; SANTOS, F. C. dos; ALBUQUERQUE FILHO, M. R. de; ALMEIDA, R. G. de; ALVES, F. V.; VILELA, L.; BARBOSA, T. A.; PINTO, C. F.; FEIJO, G. L. D.; SIMEÃO, R. M. Beef production with low carbon emission in tropical pastures: a case study for the validation of guidelines. In: CONGRESSO MUNDIAL SOBRE SISTEMAS DE INTEGRAÇÃO LAVOURA-PECUÁRIA-FLORESTA, 2., 2021. Anais... Brasília, DF: Ministério da Agricultura, Pecuária e Abastecimento: Embrapa, 2021. Evento online. 169| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 327. | | ALVES, F. V.; ALMEIDA, R. G. de; LAURA, V. A.; PORFIRIO-DA-SILVA, V.; GOMES, R. da C.; MACEDO, M. C. M.; PEREIRA, M. de A.; FERREIRA, A. D.; BUNGENSTAB, D. J. 50 perguntas, 50 respostas sobre a Carne Carbono Neutro (CCN). Brasília, DF: Embrapa, 2018 29 p. (Embrapa Gado de Corte. Documentos, 245).| Biblioteca(s): Embrapa Florestas; Embrapa Gado de Corte. |
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| 328. | | MULLER, M. D.; BARROS, I. de; SILVEIRA, J. G. da; SILVA, J. J. N. da; SANTOS, F. M.; SALTON, J. C.; VERONEZE, A. J.; ALMEIDA, R. G. de; FERREIRA, I. G. M. Adotabilidade de sistemas de integração lavoura-pecuária-floresta no Mato Grosso do Sul. Juiz de Fora: Embrapa Gado de Leite, 2023. 24 p. (Embrapa Gado de Leite. Boletim de Pesquisa e Desenvolvimento, 47). Selo ODS 2, Selo ODS 12, Selo ODS 15.| Tipo: Boletim de Pesquisa e Desenvolvimento |
| Biblioteca(s): Embrapa Agropecuária Oeste; Embrapa Gado de Corte; Embrapa Gado de Leite. |
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| 329. | | SALES, K. C.; FERREIRA, D. P.; ABREU, J. G. de; COELHO, M. S. S.; SILVA JUNIOR, C. L. S.; ALMEIDA, R. G. de; AZEVEDO, V. H. de; FERREIRA, E. A.; RODRIGUES, J. A. Animal production in piatã grass pasture in an integrated system. In: WORLD CONGRESS ON INTEGRATED CROP-LIVESTOCK-FORESTRY SYSTEMS: 100% DIGITAL, 2., 2021. WCCLF 2021 proceedings. Campo Grande, MS: Embrapa Gado de Corte, 2021. p. 365-368.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 330. | | REIS, R. H. P. dos; ABREU, J. G. de; ALMEIDA, R. G. de; CABRAL, L. da S.; BARROS, L. V. de; CABRAL, C. E. A.; BEHLING NETO, A.; MATTER, E.; ROYER, P. O.; HERRERA, D. M.; FARIAS, J. de M. Agronomic Characteristics, Chemical Composition and In vitro Gas Production of Sugarcane Cultivars (Saccharum spp.) for Feeding Ruminants. Journal of Experimental Agriculture International, v. 35, n. 1, p. 1-8, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: C - 0 |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 331. | | MONTEIRO, I. J. G.; ABREU, J. G. de; CABRAL, L. da S.; ALMEIDA, R. G. de; REIS, R. H. P. dos; BEHLING NETO, A.; CABRAL, C. E. A.; BARROS, L. V. de; AVELINO, A. C. D.; PENSO, S. Ensilagem de capim elefante acrescida de casca de soja ou farelo de arroz. Semina. ciências agrárias, Londrina, v. 37, n. 6, p. 4203-4212, nov./dez. 2016. Título em inglês: Ensiling of elephant grass with soybean hulls or rie bran.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 332. | | CORDEIRO, L. A. M.; VILELA, L.; MACEDO, M. C. M.; ZIMMER, A. H.; RAMOS, A. K. B.; BRAGA, G. J.; MACIEL, G. A.; MARCHAO, R. L.; ALMEIDA, R. G. de. Estratégias para recuperação e renovação de pastagens degradadas no Cerrado. Planaltina, DF: Embrapa Cerrados, 2022. 27 p. (Embrapa Cerrados. Documentos, 397).| Biblioteca(s): Embrapa Cerrados; Embrapa Gado de Corte. |
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| 333. | | FERREIRA, D. P.; ABREU, J. G. de; ALMEIDA, R. G. de; AZEVEDO, V. H. de; FERREIRA, E. A.; SALES, K. C.; RODRIGUES, J. A.; OLIVEIRA, E. S. B. de; OLIVEIRA NETO, V. J. de. Fiber and digestibility of Piatã grass in systems in integration. In: WORLD CONGRESS ON INTEGRATED CROP-LIVESTOCK-FORESTRY SYSTEMS: 100% DIGITAL, 2., 2021. WCCLF 2021 proceedings. Campo Grande, MS: Embrapa Gado de Corte, 2021. p. 278-282.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 334. | | QUINTINO, A. da C.; ABREU, J. G.; ALMEIDA, R. G. de; MACEDO, M. C. M.; ECHEVERRIA, D. M. S.; GAMARRA, E. L.; CARVALHO, P. H. V.; SANTOS, V. A. C. Avaliação do ph de silagens de capim-piatã e sorgo híbrido em diferentes idades de corte da forragem. In: JORNADA CIENTÍFICA DA EMBRAPA GADO DE CORTE, 6., 2010, Campo Grande, MS. Ética na pesquisa científica: [Anais da ...]. Campo Grande, MS: Embrapa Gado de Corte, 2010. 1 CD-ROM. Coordenadora: Vanessa Felipe de Souza 1 p.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 335. | | ALMEIDA, R. G. de; ALMEIDA, E, M.; GOMES, R. da C.; ALVES, F. V.; LAURA, V. A.; MACEDO, M. C. M.; COSTA, G. F.; MASTELARO, A. P.; BUNGENSTAB, D. J. Cattle production in silvopastoral systems compared to monoculture pasture in the Brazilian Cerrado. In: CONGRESO INTERNACIONAL DE SISTEMAS SILVOPASTORILES 10., 2019, Asunción. Por una produción sostenible: Libro de Actas. Asunción: CIPAV, 2019. p. 150-158| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 336. | | ALMEIDA, R. G. de; GOMES, R. da C.; PORFIRIO-DA-SILVA, V.; ALVES, F. V.; FEIJO, G. L. D.; FERREIRA, A. D.; OLIVEIRA, E. B. de; BUNGENSTAB, D. J. Carne carbono neutro: testando as diretrizes em um estudo de caso. In: SOTTA, E. D.; SAMPAIO, F. G.; GUADALUPE GALLARDO, J. V. (org.). Coletânea dos fatores de emissão e remoção de gases de efeito estufa da agricultura brasileira. Brasília, DF: MAPA: SENAR, 2020. p. 63| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Florestas; Embrapa Gado de Corte. |
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| 337. | | FREITAS, A. C. R. de; ALMEIDA, R. G. de; MACEDO, M. C. M.; SANTOS, F. C. dos; ALBUQUERQUE FILHO, M. R. de; SILVEIRA, M. C. T.; ALVES, B. J. R.; MADARI, B. E. Carbon footprint in integrated systems in the Brazilian Cerrado. In: INTERNATIONAL SYMPOSIUM ON INTEGRATED CROP-LIVESTOCK SYSTEMS, 4., CONGRESSO BRASILEIRO DE SISTEMAS INTEGRADOS DE PRODUÇÃO AGROPECUÁRIA, 3., ENCONTRO DE SISTEMAS INTEGRADOS DE PRODUÇÃO AGROPECUÁRIA NO SUL DO BRASIL, 7., Bento Gonçalves, 2023. Anais... IV ICLS.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia; Embrapa Arroz e Feijão; Embrapa Gado de Corte; Embrapa Milho e Sorgo; Embrapa Pecuária Sul. |
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| 338. | | ALVES, F. V.; ALMEIDA, R. G. de; LAURA, V. A.; PORFIRIO-DA-SILVA, V.; MACEDO, M. C. M.; MEDEIROS, S. R. de; FERREIRA, A. D.; GOMES, R. da C.; BUNGENSTAB, D. J.; REIS, M. Carbon Neutral Brazilian Beef: a sustainable concept for beef production. In: WORLD CONGRESS ON INTEGRATED CROP-LIVESTOCK-FOREST SYSTEMS; INTERNATIONAL SYMPOSIUM ON INTEGRATED CROP-LIVESTOCK SYSTEMS, 3., 2015, Brasília, DF. Towards sustainable intensification: proceedings. Brasília, DF: Embrapa, 2015. Resumo.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Florestas. |
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| 339. | | ALVES, F. V.; ALMEIDA, R. G. de; LAURA, V. A.; PORFIRIO-DA-SILVA, V.; MACEDO, M. C. M.; MEDEIROS, S. R. de; FERREIRA, A. D.; GOMES, R. da C.; BUNGENSTAB, D. J.; REIS, M. Carbon Neutral Brazilian Beef: a sustainable concept for beef production. In: WORLD CONGRESS ON INTEGRATED CROP-LIVESTOCK-FOREST SYSTEMS; INTERNATIONAL SYMPOSIUM ON INTEGRATED CROP-LIVESTOCK SYSTEMS, 3., 2015, Brasília, DF. Towards sustainable intensification: proceedings. Brasília, DF: Embrapa, 2015. 1 p.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 340. | | ALMEIDA, R. G. de; GOMES, R. da C.; PORFIRIO-DA-SILVA, V.; ALVES, F. V.; FEIJO, G. L. D.; FERREIRA, A. D.; OLIVEIRA, E. B. de; BUNGENSTAB, D. J. Carbon Neutral Brazilian Beef: testing its guidelines through a case study. In: INTERNATIONAL SYMPOSIUM ON GREENHOUSE GASES IN AGRICULTURE, 2., 2016, Campo Grande, MS. Proceedings... Brasília, DF: Embrapa, 2016. 502 p. (Embrapa Gado de Corte. Documentos, 216). Coordenador Roberto Giolo de Almeida. II SIGEE p. 277-281.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Florestas; Embrapa Gado de Corte. |
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| Registros recuperados : 388 | |
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