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Registro Completo |
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
05/02/2010 |
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Data da última atualização: |
12/03/2025 |
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Autoria: |
STANTON, J. M.; MCNICOL, C. D.; STEELE, V. |
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Título: |
Non-manual lysis of second-stage Meloidogyne juvenilis for identification of pure and mixed samples based on the polymerase chain reaction. |
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Ano de publicação: |
1998 |
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Fonte/Imprenta: |
Australasian Plant Pathology, v. 27, n. 2, p. 112-115, 1998. |
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Idioma: |
Inglês |
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Conteúdo: |
Non-manual methods oflysing single second-stage juveniles (J2s) of Meloidogyne and direct squashing of nematodes were assessed for consistency by the success of subsequent amplification of mitochondrial DNA by polymerase chain reaction. Microwave heating and boiling resulted in amplification of DNA from only 10% of J2s; treatment with proteinase K, 20%; direct squashing, 50%; and 24 h incubation in NaOH, 81%. Components of mixtures of mtDNA types could be detected consistently by DNA amplification only if they constituted at least 30% of the mixture. |
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Palavras-Chave: |
Meloidogyne juveniles. |
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Thesagro: |
Nematóide. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01126naa a2200169 a 4500
001 1656880
005 2025-03-12
008 1998 bl uuuu u00u1 u #d
100 1 $aSTANTON, J. M.
245 $aNon-manual lysis of second-stage Meloidogyne juvenilis for identification of pure and mixed samples based on the polymerase chain reaction.$h[electronic resource]
260 $c1998
520 $aNon-manual methods oflysing single second-stage juveniles (J2s) of Meloidogyne and direct squashing of nematodes were assessed for consistency by the success of subsequent amplification of mitochondrial DNA by polymerase chain reaction. Microwave heating and boiling resulted in amplification of DNA from only 10% of J2s; treatment with proteinase K, 20%; direct squashing, 50%; and 24 h incubation in NaOH, 81%. Components of mixtures of mtDNA types could be detected consistently by DNA amplification only if they constituted at least 30% of the mixture.
650 $aNematóide
653 $aMeloidogyne juveniles
700 1 $aMCNICOL, C. D.
700 1 $aSTEELE, V.
773 $tAustralasian Plant Pathology$gv. 27, n. 2, p. 112-115, 1998.
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Registro original: |
Embrapa Florestas (CNPF) |
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