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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
30/08/2007 |
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Data da última atualização: |
18/03/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FORLANO, M. D.; TEIXEIRA, K. R. dos S.; SCOFIELD, A.; ELISEI, C.; YOTOKO, K. S. C.; FERNANDES, K. R.; LINHARES, G. F. C.; EWING, S. A.; MASSARD, C. L. |
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Afiliação: |
M. D. Forlano, UCLA; Kátia Regina dos Santos Teixeira, Embrapa Agrobiologia; A. Scofield, UFPA; C. Elisei, Embrapa Gado de Corte; K. S. C. Yotoko, PUCRS; K. R. Fernandes, UFRRJ; G. F. C. Linhares, UFGO; S. A. Ewing, Oklahoma State University; C. L. Massard, UFRRJ. |
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Título: |
Molecular characterization of Hepatozoon sp. from Brazilian dogs and its phylogenetic relationship with other Hepatozoon spp. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
Veterinary Parasitology, Amsterdam, v. 145, n. 1-2, p. 21-30, apr. 2007. |
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Idioma: |
Inglês |
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Notas: |
Parceria: UCLA, Universidade Federal do Pará, Embrapa Gado de Corte, Universidade Católica do Rio Grande do Sul, UFRRJ, Universidade Federal de Goiás, Oklahoma State University |
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Conteúdo: |
To characterize phylogenetically the species which causes canine hepatozoonosis at two rural areas of Rio de Janeiro State, Brazil, we used universal or Hepatozoon spp. primer sets for the 18S SSU rRNA coding region. DNA extracts were obtained from blood samples of thirteen dogs naturally infected, from four experimentally infected, and from five puppies infected by vertical transmission from a dam, that was experimentally infected. DNA of sporozoites of Hepatozoon americanum was used as positive control. The amplification of DNA extracts from blood of dogs infected with sporozoites of Hepatozoon spp. was observed in the presence of primers to 18S SSU rRNA gene of Hepatozoon spp., whereas DNA of H. americanum sporozoites was amplified in the presence of either universal or Hepatozoon spp.-specific primer sets; the amplified products were approximately 600 bp in size. Cloned PCR products obtained from DNA extracts of blood from two dogs experimentally infected with Hepatozoon sp. were sequenced. The consensus sequence, derived from six sequence data sets, were blasted against sequences of 18S SSU rRNA of Hepatozoon spp. available at GenBank and aligned to homologous sequences to perform the phylogenetic analysis. This analysis clearly showed that our sequence clustered, independently of H. americanum sequences, within a group comprising other Hepatozoon canis sequences. Our results confirmed the hypothesis that the agent causing hepatozoonosis in the areas studied in Brazil is H. canis, supporting previous reports that were based on morphological and morphometric analyses. MenosTo characterize phylogenetically the species which causes canine hepatozoonosis at two rural areas of Rio de Janeiro State, Brazil, we used universal or Hepatozoon spp. primer sets for the 18S SSU rRNA coding region. DNA extracts were obtained from blood samples of thirteen dogs naturally infected, from four experimentally infected, and from five puppies infected by vertical transmission from a dam, that was experimentally infected. DNA of sporozoites of Hepatozoon americanum was used as positive control. The amplification of DNA extracts from blood of dogs infected with sporozoites of Hepatozoon spp. was observed in the presence of primers to 18S SSU rRNA gene of Hepatozoon spp., whereas DNA of H. americanum sporozoites was amplified in the presence of either universal or Hepatozoon spp.-specific primer sets; the amplified products were approximately 600 bp in size. Cloned PCR products obtained from DNA extracts of blood from two dogs experimentally infected with Hepatozoon sp. were sequenced. The consensus sequence, derived from six sequence data sets, were blasted against sequences of 18S SSU rRNA of Hepatozoon spp. available at GenBank and aligned to homologous sequences to perform the phylogenetic analysis. This analysis clearly showed that our sequence clustered, independently of H. americanum sequences, within a group comprising other Hepatozoon canis sequences. Our results confirmed the hypothesis that the agent causing hepatozoonosis in the areas studied in Brazil i... Mostrar Tudo |
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Thesaurus Nal: |
Hepatozoon canis. |
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Categoria do assunto: |
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Marc: |
LEADER 02505naa a2200241 a 4500
001 1629413
005 2015-03-18
008 2007 bl uuuu u00u1 u #d
100 1 $aFORLANO, M. D.
245 $aMolecular characterization of Hepatozoon sp. from Brazilian dogs and its phylogenetic relationship with other Hepatozoon spp.
260 $c2007
500 $aParceria: UCLA, Universidade Federal do Pará, Embrapa Gado de Corte, Universidade Católica do Rio Grande do Sul, UFRRJ, Universidade Federal de Goiás, Oklahoma State University
520 $aTo characterize phylogenetically the species which causes canine hepatozoonosis at two rural areas of Rio de Janeiro State, Brazil, we used universal or Hepatozoon spp. primer sets for the 18S SSU rRNA coding region. DNA extracts were obtained from blood samples of thirteen dogs naturally infected, from four experimentally infected, and from five puppies infected by vertical transmission from a dam, that was experimentally infected. DNA of sporozoites of Hepatozoon americanum was used as positive control. The amplification of DNA extracts from blood of dogs infected with sporozoites of Hepatozoon spp. was observed in the presence of primers to 18S SSU rRNA gene of Hepatozoon spp., whereas DNA of H. americanum sporozoites was amplified in the presence of either universal or Hepatozoon spp.-specific primer sets; the amplified products were approximately 600 bp in size. Cloned PCR products obtained from DNA extracts of blood from two dogs experimentally infected with Hepatozoon sp. were sequenced. The consensus sequence, derived from six sequence data sets, were blasted against sequences of 18S SSU rRNA of Hepatozoon spp. available at GenBank and aligned to homologous sequences to perform the phylogenetic analysis. This analysis clearly showed that our sequence clustered, independently of H. americanum sequences, within a group comprising other Hepatozoon canis sequences. Our results confirmed the hypothesis that the agent causing hepatozoonosis in the areas studied in Brazil is H. canis, supporting previous reports that were based on morphological and morphometric analyses.
650 $aHepatozoon canis
700 1 $aTEIXEIRA, K. R. dos S.
700 1 $aSCOFIELD, A.
700 1 $aELISEI, C.
700 1 $aYOTOKO, K. S. C.
700 1 $aFERNANDES, K. R.
700 1 $aLINHARES, G. F. C.
700 1 $aEWING, S. A.
700 1 $aMASSARD, C. L.
773 $tVeterinary Parasitology, Amsterdam$gv. 145, n. 1-2, p. 21-30, apr. 2007.
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Embrapa Agrobiologia (CNPAB) |
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| 1. |  | OLIVEIRA JÚNIOR, R. C. de. Declinação magnética. In: CURSO INTENSIVO DE HEVEICULTURA PARA TÉCNICOS AGRÍCOLAS, 5., 1981, Manaus. Coletânea de postilas. Manaus: EMBRAPA-CNPSD, 1982. p. 45.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 3. | | OLIVEIRA JUNIOR, R. C. de. A erosividade das chuvas na região de Conceição do Araguaia, PA. In: CONGRESSO LATINO AMERICANO DE CIÊNCIA DO SOLO, 13.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 1.; SIMPÓSIO BRASILEIRO SOBRE MICROBIOLOGIA DO SOLO, 4.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 6.; REUNIÃO BRASILEIRA DE MANEJO E CONSERVAÇÃO DO SOLO E DA ÁGUA, 11., 1996, Aguas de Lindoia. Solo suelo 96. Aguas de Lindoia: SBCS/SLCS, 1996. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 6. | | GALLO, J.; OLIVEIRA JUNIOR, R. C. de. Avaliação da aptidão agrícola das terras das comunidades Cuieiras, Carmelino, Itapeuá e Arimum da Resex Verde para Sempre, município Porto de Moz, PA. Espaço Científico, v. 11, n. 1/2, p. 95-114, 2010.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 7. | | GALLO, J.; OLIVEIRA JUNIOR, R. C. de. Avaliação das classes de capacidade de uso das terras das comunidades Cuieiras, Carmelino, Itapeuá e Arimum da Resex Verde para Sempre, município Porto de Moz, PA. Espaço Científico, v. 11, n. 1/2, p. 55-79, 2010.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 16. | | OLIVEIRA JUNIOR, R. C. de; KELLER, M.; CRILL, P. M. Fluxes of nitrous oxide and methane in commercial soybean, rice, and maize crops on the Santarem-Belterra plateau, Para State. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 28.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 12.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 10.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 7., 2008, Londrina. Desafios para o uso do solo com eficiência e qualidade ambiental: anais. Londrina: SBCS: Embrapa Soja: IAPAR: UEL, 2008. FertBio 2008.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| Registros recuperados : 225 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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