|
Registro Completo |
|
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
|
Data corrente: |
31/03/2008 |
|
Data da última atualização: |
20/05/2025 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
PACHECO, L. G. C.; PENA, R. R.; CASTRO, T. L. P.; DORELLA, F. A.; BAHIA, R. C.; CARMINATI, R.; FROTA, M. N. L.; OLIVEIRA, S. C.; MEYER, R.; ALVES, F. S. F.; MIYOSHI, A.; AZEVEDO, V. |
|
Afiliação: |
LUIS G. C. PACHECO, UNIVERSIDADE FEDERAL DE MINAS GERAIS; ROBERTA R. PENA, UNIVERSIDADE FEDERAL DE MINAS GERAIS; UNIVERSIDADE FEDERAL DE MINAS GERAIS; UNIVERSIDADE FEDERAL DE MINAS GERAIS; ROBSON C. BAHIA, UNIVERSIDADE FEDERAL DA BAHIA; RENATO CARMINATI, UNIVERSIDADE FEDERAL DA BAHIA; MAURÍCIO N. L. FROTA, UNIVERSIDADE ESTADUAL VALE DO ACARAÚ; SÉRGIO C. OLIVEIRA, UNIVERSIDADE FEDERAL DE MINAS GERAIS; ROBERTO MEYER, UNIVERSIDADE FEDERAL DA BAHIA; FRANCISCO SELMO FERNANDES ALVES, CNPC; ANDERSON MIYOSHI, UNIVERSIDADE FEDERAL DE MINAS GERAIS; VASCO AZEVEDO, UNIVERSIDADE FEDERAL DE MINAS GERAIS. |
|
Título: |
Multiplex PCR assay for identification of Corynebacterium pseudotuberculosis from pure cultures and for rapid detection of this pathogen in clinical samples. |
|
Ano de publicação: |
2007 |
|
Fonte/Imprenta: |
Journal of Medical Microbiology, v. 56, n. 4, p. 480-486, Apr. 2007. |
|
DOI: |
10.1099/jmm.0.46997-0 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Abstract: Corynebacterium pseudotuberculosis is the aetiological agent of caseous lymphadenitis (CLA), a debilitating disease of sheep and goats. Accurate diagnosis of CLA primarily relies on microbiological examination, followed by biochemical identification of isolates. In an effort to facilitate C. pseudotuberculosis detection, a multiplex PCR (mPCR) assay was developed targeting three genes of this bacterium: the 16S rRNA gene, rpoB and pld. This method allowed efficient identification of 40 isolates of this bacterium that had been identified previously by biochemical testing. Analysis of taxonomically related species did not generate the C. pseudotuberculosis mPCR amplification profile, thereby demonstrating the assay's specificity. As little as 1 pg of C. pseudotuberculosis genomic DNA was detected by this mPCR assay, demonstrating the sensitivity of the method. The detection limit in clinical samples was estimated to be 103 c.f.u. C. pseudotuberculosis could be detected directly in pus samples from infected sheep and goats (n=56) with a high diagnostic sensitivity (94.6 %). The developed assay significantly improves rapid C. pseudotuberculosis detection and could supersede bacteriological culture for microbiological and epidemiological diagnosis of CLA. |
|
Palavras-Chave: |
Bacterial diseases; Base eequence; CLA; Corynebacterium Infections; Molecular sequence data; PCR; RNA bacterial. |
|
Thesagro: |
Caprino; Corynebacterium Pseudotuberculosis; Linfadenite Caseosa; Ovino. |
|
Thesaurus Nal: |
Caseous lymphadenitis; Lymph nodes; Polymerase chain reaction; Sheep diseases. |
|
Categoria do assunto: |
H Saúde e Patologia |
|
Marc: |
LEADER 02618naa a2200445 a 4500
001 1533624
005 2025-05-20
008 2007 bl uuuu u00u1 u #d
024 7 $a10.1099/jmm.0.46997-0$2DOI
100 1 $aPACHECO, L. G. C.
245 $aMultiplex PCR assay for identification of Corynebacterium pseudotuberculosis from pure cultures and for rapid detection of this pathogen in clinical samples.$h[electronic resource]
260 $c2007
520 $aAbstract: Corynebacterium pseudotuberculosis is the aetiological agent of caseous lymphadenitis (CLA), a debilitating disease of sheep and goats. Accurate diagnosis of CLA primarily relies on microbiological examination, followed by biochemical identification of isolates. In an effort to facilitate C. pseudotuberculosis detection, a multiplex PCR (mPCR) assay was developed targeting three genes of this bacterium: the 16S rRNA gene, rpoB and pld. This method allowed efficient identification of 40 isolates of this bacterium that had been identified previously by biochemical testing. Analysis of taxonomically related species did not generate the C. pseudotuberculosis mPCR amplification profile, thereby demonstrating the assay's specificity. As little as 1 pg of C. pseudotuberculosis genomic DNA was detected by this mPCR assay, demonstrating the sensitivity of the method. The detection limit in clinical samples was estimated to be 103 c.f.u. C. pseudotuberculosis could be detected directly in pus samples from infected sheep and goats (n=56) with a high diagnostic sensitivity (94.6 %). The developed assay significantly improves rapid C. pseudotuberculosis detection and could supersede bacteriological culture for microbiological and epidemiological diagnosis of CLA.
650 $aCaseous lymphadenitis
650 $aLymph nodes
650 $aPolymerase chain reaction
650 $aSheep diseases
650 $aCaprino
650 $aCorynebacterium Pseudotuberculosis
650 $aLinfadenite Caseosa
650 $aOvino
653 $aBacterial diseases
653 $aBase eequence
653 $aCLA
653 $aCorynebacterium Infections
653 $aMolecular sequence data
653 $aPCR
653 $aRNA bacterial
700 1 $aPENA, R. R.
700 1 $aCASTRO, T. L. P.
700 1 $aDORELLA, F. A.
700 1 $aBAHIA, R. C.
700 1 $aCARMINATI, R.
700 1 $aFROTA, M. N. L.
700 1 $aOLIVEIRA, S. C.
700 1 $aMEYER, R.
700 1 $aALVES, F. S. F.
700 1 $aMIYOSHI, A.
700 1 $aAZEVEDO, V.
773 $tJournal of Medical Microbiology$gv. 56, n. 4, p. 480-486, Apr. 2007.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
|
