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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
12/03/2004 |
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Data da última atualização: |
18/07/2025 |
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Autoria: |
VAJTA, G.; HOLM, P.; KUWAYAMA, M.; BOOTH, P. J.; JACOBSEN, H.; GREVE, T.; CALLESEN, H. |
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Título: |
Open pulled straw (OPS) vitrification: a new way ro reduce cryoinjuries of bovine ova and embryos. |
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Ano de publicação: |
1998 |
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Fonte/Imprenta: |
Molecular Reproduction and Development, v. 51, n. 1, p. 53-58, 1998. |
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Idioma: |
Inglês |
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Conteúdo: |
Although cryopreservation of certain mammalian embryos is now a routine procedure, considerable differences of efficiency exist depending on stage, species and origin (in vivo or in vitro produced). Factors that are suspected to cause most of these differences are the amount of the intracellular lipid droplets and the different microtubular structure leading to chilling injury as well as the volume/surface ratio influencing the penetration of cryoprotectants. A new approach, the Open Pu lied Straw (OPS) method, which renders very high cooling and warming rates (over 20,000ºC/min) and short contact with concentrated cryoprotective additives (less than 30 sec over -180ºc) offers a possibility to circumvent chilling injury and to decrease toxic and osmotic damage. ln this paper we report the vitrification by the ors method of in vitro produced bovine embryos at various stages of development. Embryos cryopreserved from Day 3 to' Day 7 (Day 0 = day of fertilization) exhibited development into blastocysts at rates equivalent to those of C contrai embryos; even those cryopreserved on Day 1 è or 2 exhibited only somewhat reduced survival. Eighty-one percent of Day 8 hatched blastocysts also survived the procedure. The method was also successfully used for bovine oocytes; of 184 vitrifled oocytes, 25% developed into blastocysts after fertilization and culture for 7 days. Pregnancies were achieved following trans- fer after vitrification at bath the oocyte and blastocyst stage. The ors vitrification offers a new way to salve basic problems of reproductive cryobiology and may have practical impact on animal biotechnology and human assisted reproduction. MenosAlthough cryopreservation of certain mammalian embryos is now a routine procedure, considerable differences of efficiency exist depending on stage, species and origin (in vivo or in vitro produced). Factors that are suspected to cause most of these differences are the amount of the intracellular lipid droplets and the different microtubular structure leading to chilling injury as well as the volume/surface ratio influencing the penetration of cryoprotectants. A new approach, the Open Pu lied Straw (OPS) method, which renders very high cooling and warming rates (over 20,000ºC/min) and short contact with concentrated cryoprotective additives (less than 30 sec over -180ºc) offers a possibility to circumvent chilling injury and to decrease toxic and osmotic damage. ln this paper we report the vitrification by the ors method of in vitro produced bovine embryos at various stages of development. Embryos cryopreserved from Day 3 to' Day 7 (Day 0 = day of fertilization) exhibited development into blastocysts at rates equivalent to those of C contrai embryos; even those cryopreserved on Day 1 è or 2 exhibited only somewhat reduced survival. Eighty-one percent of Day 8 hatched blastocysts also survived the procedure. The method was also successfully used for bovine oocytes; of 184 vitrifled oocytes, 25% developed into blastocysts after fertilization and culture for 7 days. Pregnancies were achieved following trans- fer after vitrification at bath the oocyte and blastocyst stage. The or... Mostrar Tudo |
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Palavras-Chave: |
OPS; Ovocito; Vitrificação. |
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Thesagro: |
Bovino; Criopreservação; Embrião; Reprodução Animal. |
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Thesaurus Nal: |
Cattle; Cryopreservation; Research methods. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02531naa a2200313 a 4500
001 1530362
005 2025-07-18
008 1998 bl uuuu u00u1 u #d
100 1 $aVAJTA, G.
245 $aOpen pulled straw (OPS) vitrification$ba new way ro reduce cryoinjuries of bovine ova and embryos.$h[electronic resource]
260 $c1998
520 $aAlthough cryopreservation of certain mammalian embryos is now a routine procedure, considerable differences of efficiency exist depending on stage, species and origin (in vivo or in vitro produced). Factors that are suspected to cause most of these differences are the amount of the intracellular lipid droplets and the different microtubular structure leading to chilling injury as well as the volume/surface ratio influencing the penetration of cryoprotectants. A new approach, the Open Pu lied Straw (OPS) method, which renders very high cooling and warming rates (over 20,000ºC/min) and short contact with concentrated cryoprotective additives (less than 30 sec over -180ºc) offers a possibility to circumvent chilling injury and to decrease toxic and osmotic damage. ln this paper we report the vitrification by the ors method of in vitro produced bovine embryos at various stages of development. Embryos cryopreserved from Day 3 to' Day 7 (Day 0 = day of fertilization) exhibited development into blastocysts at rates equivalent to those of C contrai embryos; even those cryopreserved on Day 1 è or 2 exhibited only somewhat reduced survival. Eighty-one percent of Day 8 hatched blastocysts also survived the procedure. The method was also successfully used for bovine oocytes; of 184 vitrifled oocytes, 25% developed into blastocysts after fertilization and culture for 7 days. Pregnancies were achieved following trans- fer after vitrification at bath the oocyte and blastocyst stage. The ors vitrification offers a new way to salve basic problems of reproductive cryobiology and may have practical impact on animal biotechnology and human assisted reproduction.
650 $aCattle
650 $aCryopreservation
650 $aResearch methods
650 $aBovino
650 $aCriopreservação
650 $aEmbrião
650 $aReprodução Animal
653 $aOPS
653 $aOvocito
653 $aVitrificação
700 1 $aHOLM, P.
700 1 $aKUWAYAMA, M.
700 1 $aBOOTH, P. J.
700 1 $aJACOBSEN, H.
700 1 $aGREVE, T.
700 1 $aCALLESEN, H.
773 $tMolecular Reproduction and Development$gv. 51, n. 1, p. 53-58, 1998.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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