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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
01/09/2003 |
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Data da última atualização: |
28/08/2023 |
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Autoria: |
GOOTWINE, E.; BARASH, I.; BOR, A.; DEKEL, I.; FRIEDLER, A.; HELLER, M.; ZAHARONI, U.; ZENUE, A.; SHANI, M. |
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Título: |
Factors affecting success of embryo collection and transfer in a transgenic goat program. |
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Ano de publicação: |
1997 |
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Fonte/Imprenta: |
Theriogenology, v. 48, n. 3, p. 485-499, Aug., 1997. |
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DOI: |
10.1016/s0093-691x(97)00257-4 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: During a goat transgenic program that took place in Israel from July 1995 to February 1996, Saanen (n=343) and Nubian x Damascus (n=378) crossbred goats of mixed ages were used as donors (n=433) and recipients (n=288). The effects of season, age, number of surgical procedures, previous hormonal treatments and ovulation rate on the number of microinjectable embryos collected were studied. Likewise, the effects of these parameters on the pregnancy rate as well as the number of embryos transplanted, endogenous progesterone concentrations and exogenous progesterone supplementation were studied in recipient does. Following superovulation with ovine follicle stimulating hormone, 85% of the does responded with 13.6:t5.7 (mean +- .S D) ovulatlons/doe. Age, month and number of previous hormonal treatments significantly affected the ovulation rate. The average recovery rate was 70%, and it was affected only by the ovulation rate. Pronuciei were visualized in about 30% of the flushed embryos (including unfertilized ova), and those were microinjected with human serum albumin gene construct. About 68% of the injected embryos underwent at least one division during an overnight incubation, and those embryos were transferred, giving about 2.0 transferred embryos per ovulated donor. Of the recipients, 86% responded following synchronization with 3.1:t 1.6 (mean +- .S D) ovulations per doe. Breed and month had a significant effect on the ovulation rate. Two or three microinjected embryos were transferred to each recipient, resulting in more than a 40% pregnancy rate during September to November. Lower pregnancy rates were obtained before and after that period. By monitoring plasma progesterone concentrations In the recipients it was found that progesterone concentration was correlated with the ovulation rate. However, the pregnancy rate was not affected by progesterone concentration. During January and February, 30 to 50% of the recipients failed to develop functional corpora lutea (C L) following embryo transfer, which explained- the lower pregnancy rate In those months. Of the 86 kids bora 4 were transgenic. MenosAbstract: During a goat transgenic program that took place in Israel from July 1995 to February 1996, Saanen (n=343) and Nubian x Damascus (n=378) crossbred goats of mixed ages were used as donors (n=433) and recipients (n=288). The effects of season, age, number of surgical procedures, previous hormonal treatments and ovulation rate on the number of microinjectable embryos collected were studied. Likewise, the effects of these parameters on the pregnancy rate as well as the number of embryos transplanted, endogenous progesterone concentrations and exogenous progesterone supplementation were studied in recipient does. Following superovulation with ovine follicle stimulating hormone, 85% of the does responded with 13.6:t5.7 (mean +- .S D) ovulatlons/doe. Age, month and number of previous hormonal treatments significantly affected the ovulation rate. The average recovery rate was 70%, and it was affected only by the ovulation rate. Pronuciei were visualized in about 30% of the flushed embryos (including unfertilized ova), and those were microinjected with human serum albumin gene construct. About 68% of the injected embryos underwent at least one division during an overnight incubation, and those embryos were transferred, giving about 2.0 transferred embryos per ovulated donor. Of the recipients, 86% responded following synchronization with 3.1:t 1.6 (mean +- .S D) ovulations per doe. Breed and month had a significant effect on the ovulation rate. Two or three microinjected em... Mostrar Tudo |
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Palavras-Chave: |
Coleta; Transgênico; Transgenics. |
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Thesagro: |
Caprino; Embrião. |
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Thesaurus Nal: |
Embryo transfer; Goats; Progesterone; Reproduction; Superovulation. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 03063naa a2200349 a 4500
001 1529614
005 2023-08-28
008 1997 bl uuuu u00u1 u #d
024 7 $a10.1016/s0093-691x(97)00257-4$2DOI
100 1 $aGOOTWINE, E.
245 $aFactors affecting success of embryo collection and transfer in a transgenic goat program.$h[electronic resource]
260 $c1997
520 $aAbstract: During a goat transgenic program that took place in Israel from July 1995 to February 1996, Saanen (n=343) and Nubian x Damascus (n=378) crossbred goats of mixed ages were used as donors (n=433) and recipients (n=288). The effects of season, age, number of surgical procedures, previous hormonal treatments and ovulation rate on the number of microinjectable embryos collected were studied. Likewise, the effects of these parameters on the pregnancy rate as well as the number of embryos transplanted, endogenous progesterone concentrations and exogenous progesterone supplementation were studied in recipient does. Following superovulation with ovine follicle stimulating hormone, 85% of the does responded with 13.6:t5.7 (mean +- .S D) ovulatlons/doe. Age, month and number of previous hormonal treatments significantly affected the ovulation rate. The average recovery rate was 70%, and it was affected only by the ovulation rate. Pronuciei were visualized in about 30% of the flushed embryos (including unfertilized ova), and those were microinjected with human serum albumin gene construct. About 68% of the injected embryos underwent at least one division during an overnight incubation, and those embryos were transferred, giving about 2.0 transferred embryos per ovulated donor. Of the recipients, 86% responded following synchronization with 3.1:t 1.6 (mean +- .S D) ovulations per doe. Breed and month had a significant effect on the ovulation rate. Two or three microinjected embryos were transferred to each recipient, resulting in more than a 40% pregnancy rate during September to November. Lower pregnancy rates were obtained before and after that period. By monitoring plasma progesterone concentrations In the recipients it was found that progesterone concentration was correlated with the ovulation rate. However, the pregnancy rate was not affected by progesterone concentration. During January and February, 30 to 50% of the recipients failed to develop functional corpora lutea (C L) following embryo transfer, which explained- the lower pregnancy rate In those months. Of the 86 kids bora 4 were transgenic.
650 $aEmbryo transfer
650 $aGoats
650 $aProgesterone
650 $aReproduction
650 $aSuperovulation
650 $aCaprino
650 $aEmbrião
653 $aColeta
653 $aTransgênico
653 $aTransgenics
700 1 $aBARASH, I.
700 1 $aBOR, A.
700 1 $aDEKEL, I.
700 1 $aFRIEDLER, A.
700 1 $aHELLER, M.
700 1 $aZAHARONI, U.
700 1 $aZENUE, A.
700 1 $aSHANI, M.
773 $tTheriogenology$gv. 48, n. 3, p. 485-499, Aug., 1997.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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