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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
30/08/2002 |
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Data da última atualização: |
26/01/2024 |
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Autoria: |
MAUDET, C.; TABERLET, P. |
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Título: |
Detection of cows' milk in goats' cheeses inferred from mitochondrial DNA polymorphism. |
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Ano de publicação: |
2001 |
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Fonte/Imprenta: |
Journal of Dairey Research, v. 68, n. 2, p. 229-235, May, 2001. |
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DOI: |
https://doi.org/10.1017/S0022029901004794 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: A new polymerase chain reaction (PCR)-based method was developed to detect cows' milk in goat cheese. This method is based on mitochondrial DNA (mtDNA) control region sequence variations. DNA extractions from 150 mg of cheese were carried out using a spin column-based method. Subsequent PCR amplifications of DNA were performed with cow specific primers, demonstrating the ability to detect cows' milk in a variety of cheeses. This simple approach provides high quality DNA, and is shown to be very sensitive, with a detection limit of less than 0·1% of cows' milk. Analysis of an agarose gel digital image allows a rough estimation of the percentage of cows' milk used in adulteration. |
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Palavras-Chave: |
Cow milk; Detecção; Deteccao em PCR; DNA Mitochondrial; DNA mitocondrial; Genética mitocondrial; Leite de Vaca; PCR. |
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Thesagro: |
Bovino; Caprino; Citogenética Animal; DNA; Leite de Cabra; Polimorfismo; Queijo. |
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Thesaurus Nal: |
Adulterated products; Cattle; Cheese milk; Cheeses; Detection; Food analysis; Food chemistry; Food contamination; Genetic polymorphism; Goat cheese; Goat milk; Goats; Milk proteins; Mitochondrial DNA; Polymerase chain reaction. |
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Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02064naa a2200505 a 4500
001 1526433
005 2024-01-26
008 2001 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1017/S0022029901004794$2DOI
100 1 $aMAUDET, C.
245 $aDetection of cows' milk in goats' cheeses inferred from mitochondrial DNA polymorphism.$h[electronic resource]
260 $c2001
520 $aAbstract: A new polymerase chain reaction (PCR)-based method was developed to detect cows' milk in goat cheese. This method is based on mitochondrial DNA (mtDNA) control region sequence variations. DNA extractions from 150 mg of cheese were carried out using a spin column-based method. Subsequent PCR amplifications of DNA were performed with cow specific primers, demonstrating the ability to detect cows' milk in a variety of cheeses. This simple approach provides high quality DNA, and is shown to be very sensitive, with a detection limit of less than 0·1% of cows' milk. Analysis of an agarose gel digital image allows a rough estimation of the percentage of cows' milk used in adulteration.
650 $aAdulterated products
650 $aCattle
650 $aCheese milk
650 $aCheeses
650 $aDetection
650 $aFood analysis
650 $aFood chemistry
650 $aFood contamination
650 $aGenetic polymorphism
650 $aGoat cheese
650 $aGoat milk
650 $aGoats
650 $aMilk proteins
650 $aMitochondrial DNA
650 $aPolymerase chain reaction
650 $aBovino
650 $aCaprino
650 $aCitogenética Animal
650 $aDNA
650 $aLeite de Cabra
650 $aPolimorfismo
650 $aQueijo
653 $aCow milk
653 $aDetecção
653 $aDeteccao em PCR
653 $aDNA Mitochondrial
653 $aDNA mitocondrial
653 $aGenética mitocondrial
653 $aLeite de Vaca
653 $aPCR
700 1 $aTABERLET, P.
773 $tJournal of Dairey Research$gv. 68, n. 2, p. 229-235, May, 2001.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
