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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
21/11/1996 |
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Data da última atualização: |
12/06/2023 |
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Autoria: |
HARMACHE, A.; VITU, C.; RUSSO, P.; BOUYAC, M.; HIEBLOT, C.; PEVERI, P.; VIGNE, R.; SUZAN, M. |
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Título: |
The caprine arthritis encephalitis virus tat gene is dispensable for efficient viral replication in vitro and in vivo. |
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Ano de publicação: |
1995 |
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Fonte/Imprenta: |
Journal of Virology, v. 69, n. 9, p. 5445-5494, 1995. |
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DOI: |
10.1128/JVI.69.9.5445-5454.1995. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Caprine arthritis encephalitis virus (CAEV) is a lentivirus closely related to visna virus and more distantly to other lentiviruses, such as human immunodeficiency virus. The genomes of visna virus and CAEV contain a tat gene encoding a protein able to weakly transactivate its own long terminal repeat, suggesting that transactivation may be a dispensable function for viral replication. Three different tat gene mutants of an infectious molecular clone of CAEV were used to study their replication after transfection or infection of primary goat synovial membrane cells and of blood-derived mononuclear cells or macrophages. Our results showed no difference between replication of the wild type and either the complete tat deletion mutant or the tat stop point mutant, whereas slower growth kinetics and lower levels of expression of the partial tat deletion mutant that of the wild type were obtained in these cells. Quantitative PCR and reverse transcription-PCR analyses of the different steps of a single replicative cycle revealed an identical pattern of retrotranscription, transcription, and viral production, whereas time course analysis demonstrated that the intracellular level of viral genomic RNA was affected by the partial tat deletion at later time points. We then compared the infectious properties of the wild-type and tat mutant viruses in vivo by direct inoculation of proviral DNAs into the joints of goats. All the animals seroconverted between 27 and 70 days postinoculation. Moreover, we were able to isolate tat mutant CAEV from blood-derived macrophages that was still able to infect synovial membrane cells in vitro. This study clearly demonstrates that the tat gene of CAEV is dispensable for viral replication in vitro and in vivo. MenosAbstract: Caprine arthritis encephalitis virus (CAEV) is a lentivirus closely related to visna virus and more distantly to other lentiviruses, such as human immunodeficiency virus. The genomes of visna virus and CAEV contain a tat gene encoding a protein able to weakly transactivate its own long terminal repeat, suggesting that transactivation may be a dispensable function for viral replication. Three different tat gene mutants of an infectious molecular clone of CAEV were used to study their replication after transfection or infection of primary goat synovial membrane cells and of blood-derived mononuclear cells or macrophages. Our results showed no difference between replication of the wild type and either the complete tat deletion mutant or the tat stop point mutant, whereas slower growth kinetics and lower levels of expression of the partial tat deletion mutant that of the wild type were obtained in these cells. Quantitative PCR and reverse transcription-PCR analyses of the different steps of a single replicative cycle revealed an identical pattern of retrotranscription, transcription, and viral production, whereas time course analysis demonstrated that the intracellular level of viral genomic RNA was affected by the partial tat deletion at later time points. We then compared the infectious properties of the wild-type and tat mutant viruses in vivo by direct inoculation of proviral DNAs into the joints of goats. All the animals seroconverted between 27 and 70 days postin... Mostrar Tudo |
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Palavras-Chave: |
Base sequence; CAEV; Gene Products; Molecular Sequence Data; Protein biosynthesis; Tat Oligonucleotide Probes. |
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Thesagro: |
Caprino; Doença. |
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Thesaurus Nal: |
Caprine arthritis encephalitis virus; Enzyme-linked immunosorbent assay; Genome; Goat diseases; Goats; Oligonucleotide probes; Polymerase chain reaction; Restriction mapping; Virus replication; Visna maedi virus; Western blotting. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 03072naa a2200445 a 4500
001 1522631
005 2023-06-12
008 1995 bl uuuu u00u1 u #d
024 7 $a10.1128/JVI.69.9.5445-5454.1995.$2DOI
100 1 $aHARMACHE, A.
245 $aThe caprine arthritis encephalitis virus tat gene is dispensable for efficient viral replication in vitro and in vivo.$h[electronic resource]
260 $c1995
520 $aAbstract: Caprine arthritis encephalitis virus (CAEV) is a lentivirus closely related to visna virus and more distantly to other lentiviruses, such as human immunodeficiency virus. The genomes of visna virus and CAEV contain a tat gene encoding a protein able to weakly transactivate its own long terminal repeat, suggesting that transactivation may be a dispensable function for viral replication. Three different tat gene mutants of an infectious molecular clone of CAEV were used to study their replication after transfection or infection of primary goat synovial membrane cells and of blood-derived mononuclear cells or macrophages. Our results showed no difference between replication of the wild type and either the complete tat deletion mutant or the tat stop point mutant, whereas slower growth kinetics and lower levels of expression of the partial tat deletion mutant that of the wild type were obtained in these cells. Quantitative PCR and reverse transcription-PCR analyses of the different steps of a single replicative cycle revealed an identical pattern of retrotranscription, transcription, and viral production, whereas time course analysis demonstrated that the intracellular level of viral genomic RNA was affected by the partial tat deletion at later time points. We then compared the infectious properties of the wild-type and tat mutant viruses in vivo by direct inoculation of proviral DNAs into the joints of goats. All the animals seroconverted between 27 and 70 days postinoculation. Moreover, we were able to isolate tat mutant CAEV from blood-derived macrophages that was still able to infect synovial membrane cells in vitro. This study clearly demonstrates that the tat gene of CAEV is dispensable for viral replication in vitro and in vivo.
650 $aCaprine arthritis encephalitis virus
650 $aEnzyme-linked immunosorbent assay
650 $aGenome
650 $aGoat diseases
650 $aGoats
650 $aOligonucleotide probes
650 $aPolymerase chain reaction
650 $aRestriction mapping
650 $aVirus replication
650 $aVisna maedi virus
650 $aWestern blotting
650 $aCaprino
650 $aDoença
653 $aBase sequence
653 $aCAEV
653 $aGene Products
653 $aMolecular Sequence Data
653 $aProtein biosynthesis
653 $aTat Oligonucleotide Probes
700 1 $aVITU, C.
700 1 $aRUSSO, P.
700 1 $aBOUYAC, M.
700 1 $aHIEBLOT, C.
700 1 $aPEVERI, P.
700 1 $aVIGNE, R.
700 1 $aSUZAN, M.
773 $tJournal of Virology$gv. 69, n. 9, p. 5445-5494, 1995.
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Embrapa Caprinos e Ovinos (CNPC) |
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| 2. |  | ARAÚJO, I. C.; COSTA, I. C. G.; CHAVES, M. H.; ARAÚJO, E. C. E.; MONTE, F. J. Q.; NASCIMENTO, R. F. do. Teor de óleo e composição de ácidos graxos em acessos de pinhão manso. In: CONGRESSO DA REDE BRASILEIRA DE TECNOLOGIA DE BIODIESEL, 3., 2009, Brasília, DF. Anais... Brasília, DF: MCT: MBC, 2009. v. 3, p. 603-604| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Meio-Norte. |
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| 3. | | ARAÚJO, I. C.; COSTA, I. C. G.; PEREIRA, D. L. A.; DANTAS, A. F.; CHAVES, M. H.; CAVALCANTE, A. A. C. M.; ARAÚJO, E. C. E. Estudos tóxicos, citotóxicos e genotóxicos de lipídios de pinhão manso (Jatropha curcas L.) em meristemas de raízes de Allium cepa. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE QUÍMICA, 32., 2009, Fortaleza. Químicos para uma potência emergente: resumos. São Paulo: Sociedade Brasileira de Química, 2009. 1 p.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Meio-Norte. |
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| 4. | | ARAÚJO, I. C.; COSTA, I. C. G.; CHAVES, M. H.; MOURA, C. V. R. de; ARAUJO, E. C. E.; AUED-PIMENTEL, S.; CARUSO, M. S. F. Caracterização do óleo de pinhão-manso (Jatropha curcas). In: FEITOSA, C. M.; MATOS, J. M. E. de (Org.). Óleos e gorduras: aspectos químicos, biológicos e farmacológicos. Campinas: Editora Átomo, 2022. Cap. 7, p. 109-121.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Meio-Norte. |
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| Registros recuperados : 4 | |
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