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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
12/02/1996 |
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Data da última atualização: |
13/06/2023 |
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Autoria: |
THOMPSON, J. G.; BELL, A. C. S.; PUGH, P. A.; TERVIT, H. R. |
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Título: |
Metabolism of pyruvate by pre-elongation sheep embryos and effect of pyruvate and lactate concentrations during culture in vitro. |
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Ano de publicação: |
1993 |
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Fonte/Imprenta: |
Reproduction, Fertility and Development, v. 5, n. 4, p. 417-423, 1993. |
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DOI: |
10.1071/rd9930417. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: In the first of two experiments, utilization of [1-14C]pyruvate by 8-cell and blastocyst-stage embryos derived in vivo was examined during a 3-h incubation in HEPES-buffered synthetic oviduct fluid (SOF) medium in the presence or absence of other substrates. In the second, a factorial design examined the effect of pyruvate (0, 0.33, 1.0 and 3.3 mM) and lactate (3.3, 10 and 33 mM) on development of 1- and 2-cell sheep embryos cultured in vitro in a modified SOF medium (containing glucose, glutamine and modified Eagle's medium non-essential amino acids). Peak utilization of [1-14C]pyruvate was unaffected by the presence or absence of other energy substrates. In contrast, rate of utilization was affected by the addition of other energy substrates, with half maximal utilization occurring at either 0.4 +/- 0.2 mM or 1.2 +/- 0.2 mM for 8-cells and either 0.2 +/- 0.2 mM or 1.3 +/- 0.3 mM for blastocysts when incubated in the absence or presence of other energy substrates respectively. In the second experiment the proportion of embryos developing to blastocysts was inhibited by high lactate levels (P < 0.001), but was generally not affected by pyruvate concentration. However, there was a significant interaction (P < 0.001) between pyruvate and lactate when both were present in the medium. At 0.33 mM pyruvate, 3.3 mM lactate supported good development (83 +/- 8% blastocysts) whereas 10 mM lactate supported less development (50 +/- 11%). However, at the higher levels of pyruvate this effect was lost. MenosAbstract: In the first of two experiments, utilization of [1-14C]pyruvate by 8-cell and blastocyst-stage embryos derived in vivo was examined during a 3-h incubation in HEPES-buffered synthetic oviduct fluid (SOF) medium in the presence or absence of other substrates. In the second, a factorial design examined the effect of pyruvate (0, 0.33, 1.0 and 3.3 mM) and lactate (3.3, 10 and 33 mM) on development of 1- and 2-cell sheep embryos cultured in vitro in a modified SOF medium (containing glucose, glutamine and modified Eagle's medium non-essential amino acids). Peak utilization of [1-14C]pyruvate was unaffected by the presence or absence of other energy substrates. In contrast, rate of utilization was affected by the addition of other energy substrates, with half maximal utilization occurring at either 0.4 +/- 0.2 mM or 1.2 +/- 0.2 mM for 8-cells and either 0.2 +/- 0.2 mM or 1.3 +/- 0.3 mM for blastocysts when incubated in the absence or presence of other energy substrates respectively. In the second experiment the proportion of embryos developing to blastocysts was inhibited by high lactate levels (P < 0.001), but was generally not affected by pyruvate concentration. However, there was a significant interaction (P < 0.001) between pyruvate and lactate when both were present in the medium. At 0.33 mM pyruvate, 3.3 mM lactate supported good development (83 +/- 8% blastocysts) whereas 10 mM lactate supported less development (50 +/- 11%). However, at the higher levels of pyru... Mostrar Tudo |
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Palavras-Chave: |
Biotecnlogia de embriao; Cultivo; Drug effects; Embryonic and Fetal Development; Fertilizacao in vitro; In Vitro Techniques; Pyruvates. |
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Thesagro: |
Ovino. |
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Thesaurus Nal: |
Blastocyst; Kinetics; Lactates; Lactic acid; Pharmacology; Pregnancy; Pyruvic acid; Sheep. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02580naa a2200361 a 4500
001 1522402
005 2023-06-13
008 1993 bl uuuu u00u1 u #d
024 7 $a10.1071/rd9930417.$2DOI
100 1 $aTHOMPSON, J. G.
245 $aMetabolism of pyruvate by pre-elongation sheep embryos and effect of pyruvate and lactate concentrations during culture in vitro.$h[electronic resource]
260 $c1993
520 $aAbstract: In the first of two experiments, utilization of [1-14C]pyruvate by 8-cell and blastocyst-stage embryos derived in vivo was examined during a 3-h incubation in HEPES-buffered synthetic oviduct fluid (SOF) medium in the presence or absence of other substrates. In the second, a factorial design examined the effect of pyruvate (0, 0.33, 1.0 and 3.3 mM) and lactate (3.3, 10 and 33 mM) on development of 1- and 2-cell sheep embryos cultured in vitro in a modified SOF medium (containing glucose, glutamine and modified Eagle's medium non-essential amino acids). Peak utilization of [1-14C]pyruvate was unaffected by the presence or absence of other energy substrates. In contrast, rate of utilization was affected by the addition of other energy substrates, with half maximal utilization occurring at either 0.4 +/- 0.2 mM or 1.2 +/- 0.2 mM for 8-cells and either 0.2 +/- 0.2 mM or 1.3 +/- 0.3 mM for blastocysts when incubated in the absence or presence of other energy substrates respectively. In the second experiment the proportion of embryos developing to blastocysts was inhibited by high lactate levels (P < 0.001), but was generally not affected by pyruvate concentration. However, there was a significant interaction (P < 0.001) between pyruvate and lactate when both were present in the medium. At 0.33 mM pyruvate, 3.3 mM lactate supported good development (83 +/- 8% blastocysts) whereas 10 mM lactate supported less development (50 +/- 11%). However, at the higher levels of pyruvate this effect was lost.
650 $aBlastocyst
650 $aKinetics
650 $aLactates
650 $aLactic acid
650 $aPharmacology
650 $aPregnancy
650 $aPyruvic acid
650 $aSheep
650 $aOvino
653 $aBiotecnlogia de embriao
653 $aCultivo
653 $aDrug effects
653 $aEmbryonic and Fetal Development
653 $aFertilizacao in vitro
653 $aIn Vitro Techniques
653 $aPyruvates
700 1 $aBELL, A. C. S.
700 1 $aPUGH, P. A.
700 1 $aTERVIT, H. R.
773 $tReproduction, Fertility and Development$gv. 5, n. 4, p. 417-423, 1993.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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