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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
01/08/1992 |
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Data da última atualização: |
27/04/2023 |
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Autoria: |
DOTT, H. M.; WALTON, A. |
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Título: |
Effects of dilution and washing on ram spermatozoa studied by the flow dialysis technique. |
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Ano de publicação: |
1960 |
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Fonte/Imprenta: |
Journal of Reproduction and Fertility, v. 1, n. 4, p. 350-367, 1960. |
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DOI: |
10.1530/jrf.0.0010350 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: A 'flow dialysis' technique is described which enables spermatozoa to be maintained in an active state for prolonged periods of time, under conditions of constant temperature, gaseous partial pressure, pH, osmotic pressure and sperm density. The impedance-change frequency is used to assess sperm activity. The effects of centrifugation, dilution and washing of ram spermatozoa on their activity have been studied. Washing has been shown to produce a detrimental effect on sperm activity. This effect was most pronounced when a high degree of dilution was employed at each stage of the washing procedure. Washing produced a greater decrease in the activity due to aerobic endogenous respiration than in that due to anaerobic fructolysis. Other effects of washing included an increase in the proportion of 'eosinophilic' spermatozoa and an agglutination of motile spermatozoa. An attempt was made to assess the efficiency of the washing procedure by the use of fructose, [32P]phosphate and iodinated [131I]protein as indicators. The effect of dilution on spermatozoa depends on at least three factors: (i) the degree of dilution, (ii) the rate of dilution, and (iii) the composition of the diluent. |
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Palavras-Chave: |
Diluidor; Fluxo; Tecnologia do sêmen. |
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Thesagro: |
Diálise; Espermatozóide; Ovino; Reprodução Animal. |
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Thesaurus Nal: |
Dialysis; Rams; Reproduction; Sheep; Spermatozoa. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 01981naa a2200289 a 4500
001 1521629
005 2023-04-27
008 1960 bl uuuu u00u1 u #d
024 7 $a10.1530/jrf.0.0010350$2DOI
100 1 $aDOTT, H. M.
245 $aEffects of dilution and washing on ram spermatozoa studied by the flow dialysis technique.
260 $c1960
520 $aAbstract: A 'flow dialysis' technique is described which enables spermatozoa to be maintained in an active state for prolonged periods of time, under conditions of constant temperature, gaseous partial pressure, pH, osmotic pressure and sperm density. The impedance-change frequency is used to assess sperm activity. The effects of centrifugation, dilution and washing of ram spermatozoa on their activity have been studied. Washing has been shown to produce a detrimental effect on sperm activity. This effect was most pronounced when a high degree of dilution was employed at each stage of the washing procedure. Washing produced a greater decrease in the activity due to aerobic endogenous respiration than in that due to anaerobic fructolysis. Other effects of washing included an increase in the proportion of 'eosinophilic' spermatozoa and an agglutination of motile spermatozoa. An attempt was made to assess the efficiency of the washing procedure by the use of fructose, [32P]phosphate and iodinated [131I]protein as indicators. The effect of dilution on spermatozoa depends on at least three factors: (i) the degree of dilution, (ii) the rate of dilution, and (iii) the composition of the diluent.
650 $aDialysis
650 $aRams
650 $aReproduction
650 $aSheep
650 $aSpermatozoa
650 $aDiálise
650 $aEspermatozóide
650 $aOvino
650 $aReprodução Animal
653 $aDiluidor
653 $aFluxo
653 $aTecnologia do sêmen
700 1 $aWALTON, A.
773 $tJournal of Reproduction and Fertility$gv. 1, n. 4, p. 350-367, 1960.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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