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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
01/08/1992 |
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Data da última atualização: |
23/05/2023 |
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Autoria: |
SALAMONSEN, L. A.; JONAS, H. A.; BURGER, H. G.; BUCKMASTER, J. M.; CHAMLEY, W. A.; CUMMING, I. A.; FINDLAY, J. K.; GODING, J. R. |
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Título: |
A heterologous radioimmunoassay for follicle-stimulating hormone: application to measurement of FSH in the ovine estrous cycle and in several other species including man. |
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Ano de publicação: |
1973 |
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Fonte/Imprenta: |
Endocrinology, v. 93, n. 3, p. 610-618, Sept. 1973. |
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DOI: |
10.1210/endo-93-3-610. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: A heterologous radioimmunoassay system, using an antiserum to human FSH in combination with radioiodinated ovine FSH was highly specific for FSH for a number of species, and was used to measure plasma FSH levels in sheep, rat and man in a range of physiological states. Within-assay coefficient of variation (CV) was <10% over the range 35?500 ng FSH/ml and between-assay CV was 15%. Sensitivity was 1.3 ng NIH-FSH-S6 per tube and mean recovery of FSH added to plasma was 98%. Specificity was demonstrated by the finding of parallel inhibition curves between serial dilutions of plasma from an oophorectomised ewe and standard ovine FSH, and by the failure of ovine LH, GH, prolactin and albumin, human LH, TSH, hCG, bovine LH and GnRH to show significant inhibition. Ewes were bled at 2-hr intervals for 4 days over various stages of the estrous cycle and the samples assayed for FSH and LH. Sharp peaks of FSH, ranging from 80?150 ng/ml NIH-FSH-S6 were detected at estrus, coinciding with the preovulatory LH peak. For the remainder of the cycle, FSH appeared to be secreted as surges of variable duration with short troughs and extended peaks ranging from less than 15 ng/ml to 110 ng/ml. During the luteal phase plasma LH remained at basal levels and appeared to be regulated independently of FSH. |
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Palavras-Chave: |
FSH; Immune sera; Iodine Isotopes. |
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Thesagro: |
Ciclo Estral; Endocrinologia; Fisiologia; Hormônio; Ovino; Reprodução. |
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Thesaurus Nal: |
Cattle; Chorionic gonadotropins; Estrus; Females; Follicle-stimulating hormone; Humans; Luteinizing hormone; Males; Pharmacology; Pregnancy; Prolactin; Radioimmunoassays; Rats; Reproduction; Serum albumin; Sheep; Thyrotropin. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02770naa a2200529 a 4500
001 1521023
005 2023-05-23
008 1973 bl uuuu u00u1 u #d
024 7 $a10.1210/endo-93-3-610.$2DOI
100 1 $aSALAMONSEN, L. A.
245 $aA heterologous radioimmunoassay for follicle-stimulating hormone$bapplication to measurement of FSH in the ovine estrous cycle and in several other species including man.$h[electronic resource]
260 $c1973
520 $aAbstract: A heterologous radioimmunoassay system, using an antiserum to human FSH in combination with radioiodinated ovine FSH was highly specific for FSH for a number of species, and was used to measure plasma FSH levels in sheep, rat and man in a range of physiological states. Within-assay coefficient of variation (CV) was <10% over the range 35?500 ng FSH/ml and between-assay CV was 15%. Sensitivity was 1.3 ng NIH-FSH-S6 per tube and mean recovery of FSH added to plasma was 98%. Specificity was demonstrated by the finding of parallel inhibition curves between serial dilutions of plasma from an oophorectomised ewe and standard ovine FSH, and by the failure of ovine LH, GH, prolactin and albumin, human LH, TSH, hCG, bovine LH and GnRH to show significant inhibition. Ewes were bled at 2-hr intervals for 4 days over various stages of the estrous cycle and the samples assayed for FSH and LH. Sharp peaks of FSH, ranging from 80?150 ng/ml NIH-FSH-S6 were detected at estrus, coinciding with the preovulatory LH peak. For the remainder of the cycle, FSH appeared to be secreted as surges of variable duration with short troughs and extended peaks ranging from less than 15 ng/ml to 110 ng/ml. During the luteal phase plasma LH remained at basal levels and appeared to be regulated independently of FSH.
650 $aCattle
650 $aChorionic gonadotropins
650 $aEstrus
650 $aFemales
650 $aFollicle-stimulating hormone
650 $aHumans
650 $aLuteinizing hormone
650 $aMales
650 $aPharmacology
650 $aPregnancy
650 $aProlactin
650 $aRadioimmunoassays
650 $aRats
650 $aReproduction
650 $aSerum albumin
650 $aSheep
650 $aThyrotropin
650 $aCiclo Estral
650 $aEndocrinologia
650 $aFisiologia
650 $aHormônio
650 $aOvino
650 $aReprodução
653 $aFSH
653 $aImmune sera
653 $aIodine Isotopes
700 1 $aJONAS, H. A.
700 1 $aBURGER, H. G.
700 1 $aBUCKMASTER, J. M.
700 1 $aCHAMLEY, W. A.
700 1 $aCUMMING, I. A.
700 1 $aFINDLAY, J. K.
700 1 $aGODING, J. R.
773 $tEndocrinology$gv. 93, n. 3, p. 610-618, Sept. 1973.
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Embrapa Caprinos e Ovinos (CNPC) |
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| Registros recuperados : 4 | |
| 1. |  | PELAEZ, R. R.; MENDES, T. D.; PACHECO, T. F.; URBEN, A. F.; BRASIL, B. dos S. A. F.; HELM, C. V.; MENDONCA, S.; SIQUEIRA, F. G. Enhancing enzymatic hydrolysis of sugarcane bagasse by using fungal enzymes produced in monoculture and co-culture submerse fermentation. In: SYMPOSIUM ON BIOTECHNOLOGY FOR FUELS AND CHEMICALS, 39., 2017, São Francisco, CA. [Proceedings ...]. Fairfax: Society for Industrial Microbiology and Biotechnology, 2017.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 2. | | PELÁEZ, R. R.; CAMELINI, C. M.; URBEN, A. F.; HELM, C. V.; MENDES, T. D.; MENDONCA, S.; SIQUEIRA, F. G. de. Lignocelulases de basidiomicetos cultivados em biomassas vegetais residuais da agroindústria do dendê. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 3., 2016, Brasília, DF. Anais ... Brasília, DF: Embrapa, 2016. p. 130-137.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
|  |
| 3. | | PELÁEZ, R. R.; CAMELINI, C. M.; URBEN, A. F.; HELM, C. V.; MENDES, T. D.; MENDONCA, S.; SIQUEIRA, F. G. de. Lignocelulases de basidiomicetos cultivados em biomassas vegetais residuais da agroindústria do dendê. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 3., 2016, Brasília, DF. Anais ... Brasília, DF: Embrapa, 2016. p. 130-137.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Florestas. |
| |
| 4. | | PELÁEZ, R. R.; CAMELINI, C. M.; URBEN, A. F.; HELM, C. V.; MENDES, T. D.; MENDONCA, S.; SIQUEIRA, F. G. de. Lignocelulases de basidiomicetos cultivados em biomassas vegetais residuais da agroindústria do dendê. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 3., 2016, Brasília, DF. Anais ... Brasília, DF: Embrapa, 2016. p. 130-137.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| Registros recuperados : 4 | |
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