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Registro Completo |
Biblioteca(s): |
Embrapa Milho e Sorgo. |
Data corrente: |
24/01/2006 |
Data da última atualização: |
29/05/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SELDIN, L.; ROSADO, A. S.; CRUZ, D. W. da; NOBREGA, A.; ELSAS, J. D. van; PAIVA, E. |
Afiliação: |
EMBRAPA-CNPMS. |
Título: |
Comparison of Paenibacillus azotofixans strains isolated from rhizoplane, rhizosphere, and non-root-associated soil from maize planted in two different Brazilian soils. |
Ano de publicação: |
1998 |
Fonte/Imprenta: |
Applied and Environmental Microbiology, Washington, v. 64, n. 10, p. 3860-3868, 1998. |
Idioma: |
Inglês |
Conteúdo: |
Paenibacillus azotofixans is a nitrogen-fixing bacterium often found in soil and in the rhizospheres of different grasses. In this study, two Brazilian clay soils were planted with cross-hybrid maize (BR-201) and four stages of plant growth were analysed to characterize the P. azotofixans populations present in the rhizoplanes, rhizospheres, and non-root-associated soils (herein called nonrhizospheres). A total of 106 strains were isolated and identified as P. azotofixans with an API 50CH kit, by classical biochemical tests, and via the use of specific primers based on the 16S rRNA gene in PCRs. To compare the isolated strains, phenotypic characteristics were determined and three different probes were used in hybridization experiments: two nif probes and one probe comprising a 0.58-kb fragment cloned from the P. azotofixans C3L4 genome. These results were used to construct a dendrogram, in which two main clusters could be observed. One cluster contained exclusively strains from Varzea soil, and the other contained the majority of strains from cerrado soil. The 60 strains from Varzea soil and the 46 strains from cerrado soil were further analysed with REP and BOX primers, respectively. Based on the patterns obtained, it was possible to identify 21 different groups among strains from Varzea soil and 4 different groups among strains from cerrado soil. These different patterns were tested by multivariate analysis of variance, and differences in the populations of P. azotofixans during the four stages of plant growth were demonstrated. Moreover, strains isolated from the rhizoplanes, rhizospheres, and nonrhizospheres of maize planted in cerrado and Varzea soils were shown to be statistically different; the diversity of P. azotofixans strains was affected by the soil type. MenosPaenibacillus azotofixans is a nitrogen-fixing bacterium often found in soil and in the rhizospheres of different grasses. In this study, two Brazilian clay soils were planted with cross-hybrid maize (BR-201) and four stages of plant growth were analysed to characterize the P. azotofixans populations present in the rhizoplanes, rhizospheres, and non-root-associated soils (herein called nonrhizospheres). A total of 106 strains were isolated and identified as P. azotofixans with an API 50CH kit, by classical biochemical tests, and via the use of specific primers based on the 16S rRNA gene in PCRs. To compare the isolated strains, phenotypic characteristics were determined and three different probes were used in hybridization experiments: two nif probes and one probe comprising a 0.58-kb fragment cloned from the P. azotofixans C3L4 genome. These results were used to construct a dendrogram, in which two main clusters could be observed. One cluster contained exclusively strains from Varzea soil, and the other contained the majority of strains from cerrado soil. The 60 strains from Varzea soil and the 46 strains from cerrado soil were further analysed with REP and BOX primers, respectively. Based on the patterns obtained, it was possible to identify 21 different groups among strains from Varzea soil and 4 different groups among strains from cerrado soil. These different patterns were tested by multivariate analysis of variance, and differences in the populations of P. azotofixans ... Mostrar Tudo |
Palavras-Chave: |
Maize. |
Thesaurus Nal: |
cerrado soils; clay soils; rhizosphere; soil types. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02576naa a2200241 a 4500 001 1489147 005 2018-05-29 008 1998 bl uuuu u00u1 u #d 100 1 $aSELDIN, L. 245 $aComparison of Paenibacillus azotofixans strains isolated from rhizoplane, rhizosphere, and non-root-associated soil from maize planted in two different Brazilian soils.$h[electronic resource] 260 $c1998 520 $aPaenibacillus azotofixans is a nitrogen-fixing bacterium often found in soil and in the rhizospheres of different grasses. In this study, two Brazilian clay soils were planted with cross-hybrid maize (BR-201) and four stages of plant growth were analysed to characterize the P. azotofixans populations present in the rhizoplanes, rhizospheres, and non-root-associated soils (herein called nonrhizospheres). A total of 106 strains were isolated and identified as P. azotofixans with an API 50CH kit, by classical biochemical tests, and via the use of specific primers based on the 16S rRNA gene in PCRs. To compare the isolated strains, phenotypic characteristics were determined and three different probes were used in hybridization experiments: two nif probes and one probe comprising a 0.58-kb fragment cloned from the P. azotofixans C3L4 genome. These results were used to construct a dendrogram, in which two main clusters could be observed. One cluster contained exclusively strains from Varzea soil, and the other contained the majority of strains from cerrado soil. The 60 strains from Varzea soil and the 46 strains from cerrado soil were further analysed with REP and BOX primers, respectively. Based on the patterns obtained, it was possible to identify 21 different groups among strains from Varzea soil and 4 different groups among strains from cerrado soil. These different patterns were tested by multivariate analysis of variance, and differences in the populations of P. azotofixans during the four stages of plant growth were demonstrated. Moreover, strains isolated from the rhizoplanes, rhizospheres, and nonrhizospheres of maize planted in cerrado and Varzea soils were shown to be statistically different; the diversity of P. azotofixans strains was affected by the soil type. 650 $acerrado soils 650 $aclay soils 650 $arhizosphere 650 $asoil types 653 $aMaize 700 1 $aROSADO, A. S. 700 1 $aCRUZ, D. W. da 700 1 $aNOBREGA, A. 700 1 $aELSAS, J. D. van 700 1 $aPAIVA, E. 773 $tApplied and Environmental Microbiology, Washington$gv. 64, n. 10, p. 3860-3868, 1998.
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Embrapa Milho e Sorgo (CNPMS) |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
Data corrente: |
07/03/2015 |
Data da última atualização: |
12/03/2015 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
SOUZA, D. T.; SILVA, F. P.; PANSA, C. C.; KAVAMURA, V. N.; TAKETANI, R. G.; MELO, I. S. de. |
Afiliação: |
Danilo Tosta Souza, ESALQ-USP; Fábio Sérgio Paulino da Silva, ESALQ-USP; Camila Cristiane Pansa, Fundação Herminio Ometto; Vanessa Nessner Kavamura, ESALQ-USP; Rodrigo Gouvêa Taketani, Bolsista de Pós-doutorado; ITAMAR SOARES DE MELO, CNPMA. |
Título: |
Diversidade e composição microbiana em esponjas marinhas do Arquipélago de São Pedro e São Paulo. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
Hechos Microbiológicos, v. 5, n. 2, p. 100, 2014. Suplemento. Edição das Memorias do 22º Congreso Latinoamericano de Microbiologia e 4º Congreso Colombiano de Microbiologia, Cartagena, 2014. Ref. TLP-183. |
Idioma: |
Português |
Palavras-Chave: |
Comunidade microbiana; Esponjas marinhas. |
Categoria do assunto: |
V Taxonomia de Organismos |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/120302/1/2014RA-107.pdf
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Marc: |
LEADER 00750nam a2200181 a 4500 001 2010903 005 2015-03-12 008 2014 bl uuuu u00u1 u #d 100 1 $aSOUZA, D. T. 245 $aDiversidade e composição microbiana em esponjas marinhas do Arquipélago de São Pedro e São Paulo.$h[electronic resource] 260 $aHechos Microbiológicos, v. 5, n. 2, p. 100, 2014. Suplemento. Edição das Memorias do 22º Congreso Latinoamericano de Microbiologia e 4º Congreso Colombiano de Microbiologia, Cartagena, 2014. Ref. TLP-183.$c2014 653 $aComunidade microbiana 653 $aEsponjas marinhas 700 1 $aSILVA, F. P. 700 1 $aPANSA, C. C. 700 1 $aKAVAMURA, V. N. 700 1 $aTAKETANI, R. G. 700 1 $aMELO, I. S. de
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