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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
10/12/2024 |
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Data da última atualização: |
10/12/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
AYMÉE, L.; REIS, L.; SOARES, A. C.; SOUZA, G. N. de; LILENBAUM, W. |
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Afiliação: |
LUIZA AYMÉE, UNIVERSIDADE FEDERAL FLUMINENSE; LUIZA REIS, UNIVERSIDADE FEDERAL FLUMINENSE; ANA CLARA SOARES, UNIVERSIDADE FEDERAL FLUMINENSE; GUILHERME NUNES DE SOUZA, CNPGL; WALTER LILENBAUM, UNIVERSIDADE FEDERAL FLUMINENSE. |
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Título: |
Detecting Leptospira spp. infection in cows by PCR: what is the best sample to test? |
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Ano de publicação: |
2025 |
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Fonte/Imprenta: |
Theriogenology, v. 231, p. 154-159, 2025. |
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DOI: |
https://doi.org/10.1016/j.theriogenology.2024.10.020 |
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Idioma: |
Inglês |
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Conteúdo: |
Bovine leptospirosis is a major reproductive disease. As cows can be leptospiral carriers both on the renal and genital tract, diagnosis can be challenging, with an underlying risk of misdiagnosis. Traditionally, the infection has been diagnosed by culturing or PCR from urine samples. Nevertheless, recent studies have suggested testing genital samples rather than urine, particularly for the diagnosis of genital colonization and reproductive disor ders. The present study aimed to compare urine versus genital samples to detect leptospiral carriers in naturally infected cows with poor reproductive performance under field conditions. Five herds presenting >20 % of seroreactive animals against the Sejroe serogroup were selected. Of these, 106 cows with poor reproductive performance were studied, and urine, uterine fragment (UF), and cervicovaginal mucus (CVM) were obtained and tested by lipL32-PCR. A total of 73 (68.9 %) cows were infected; 64 of which (87.7 %) were diagnosed via positive genital samples (UF and/or CVM), while only 14 (19.2 %) by urine (p ≤ 0.001). Therefore, if the study had been limited to urine samples, as largely recommended, less than 20 % of the infected cows would have been detected, representing a huge misdiagnosis of the disease that could undermine the efficacy of control programs. In this context, the present study reinforces prior findings that testing genital samples, particularly CVM, is crucial to effectively diagnosing infected subfertile cows. |
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Palavras-Chave: |
Muco cervicovaginal. |
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Thesagro: |
Bactéria; Infecção; Leptospirose; Útero. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02201naa a2200241 a 4500
001 2170235
005 2024-12-10
008 2025 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.theriogenology.2024.10.020$2DOI
100 1 $aAYMÉE, L.
245 $aDetecting Leptospira spp. infection in cows by PCR$bwhat is the best sample to test?$h[electronic resource]
260 $c2025
520 $aBovine leptospirosis is a major reproductive disease. As cows can be leptospiral carriers both on the renal and genital tract, diagnosis can be challenging, with an underlying risk of misdiagnosis. Traditionally, the infection has been diagnosed by culturing or PCR from urine samples. Nevertheless, recent studies have suggested testing genital samples rather than urine, particularly for the diagnosis of genital colonization and reproductive disor ders. The present study aimed to compare urine versus genital samples to detect leptospiral carriers in naturally infected cows with poor reproductive performance under field conditions. Five herds presenting >20 % of seroreactive animals against the Sejroe serogroup were selected. Of these, 106 cows with poor reproductive performance were studied, and urine, uterine fragment (UF), and cervicovaginal mucus (CVM) were obtained and tested by lipL32-PCR. A total of 73 (68.9 %) cows were infected; 64 of which (87.7 %) were diagnosed via positive genital samples (UF and/or CVM), while only 14 (19.2 %) by urine (p ≤ 0.001). Therefore, if the study had been limited to urine samples, as largely recommended, less than 20 % of the infected cows would have been detected, representing a huge misdiagnosis of the disease that could undermine the efficacy of control programs. In this context, the present study reinforces prior findings that testing genital samples, particularly CVM, is crucial to effectively diagnosing infected subfertile cows.
650 $aBactéria
650 $aInfecção
650 $aLeptospirose
650 $aÚtero
653 $aMuco cervicovaginal
700 1 $aREIS, L.
700 1 $aSOARES, A. C.
700 1 $aSOUZA, G. N. de
700 1 $aLILENBAUM, W.
773 $tTheriogenology$gv. 231, p. 154-159, 2025.
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| 1. |  | ROCHA-SILVA, M.; SILVA, R. S.; AZEVEDO, D. M. M. R.; SILVA, D. L. S.; OLIVEIRA, S. T. L. de; SILVA, C. I. G. R.; SILVA, M. R. da; LIRA, M. do L. da C. Efeito do ambiente seco e chuvoso sobre a etologia de vacas leiteiras. Nativa, Sinop, v. 11, n. 4, p. 577-581, 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 4 |
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