| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
|
Data corrente: |
23/04/2024 |
|
Data da última atualização: |
23/04/2024 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
RIZZONI, L. B.; PALHÃO, M. P.; VIANA, J. H. M.; SOUZA, V. O.; NASCIMENTO NETO, J. da P.; NEVES, J. P. |
|
Afiliação: |
LEANDRO BECALETE RIZZONI, UNIVERSIDADE PROFESSOR EDSON ANTÔNIO VELANO; MILLER PEREIRA PALHÃO, UNIVERSIDADE FEDERAL DE LAVRAS; JOAO HENRIQUE MOREIRA VIANA, CENARGEN; VINÍCIUS OLIVEIRA SOUZA, UNIVERSIDADE PROFESSOR EDSON ANTÔNIO VELANO; JOSÉ DA PASCOA NASCIMENTO NETO, UNIVERSIDADE FEDERAL DE LAVRAS; JAIRO PEREIRA NEVES, UNIVERSIDADE PROFESSOR EDSON ANTÔNIO VELANO. |
|
Título: |
How early can nonpregnant ewes be detected based on the blood flow of the corpus luteum? |
|
Ano de publicação: |
2024 |
|
Fonte/Imprenta: |
Small Ruminant Research, v. 232, 107211, 2024. |
|
DOI: |
https://doi.org/10.1016/j.smallrumres.2024.107211 |
|
Idioma: |
Inglês |
|
Conteúdo: |
The study aimed to characterize morpho-functional changes in corpus luteum (CL) in pregnant and non-pregnant sheep, propose diagnostic criteria for non-pregnancy, and assess diagnostic accuracy around the expected luteolysis period. Crossbred ewes (n = 34) underwent estrous synchronization, divided into G1 (unmated, n = 20) and G2 (mated, n = 14). The day of ovulation was considered as day 0 (D0). Luteal dynamic was monitored from D0 to D17, with blood samples collected every 72 h for progesterone (P4). Pregnancy was definitively diagnosed at day 30. Analyses included CL area (CLA), CL blood flow (CLBF), CLBF:CLA ratio, and serum P4. Statistical models considered group, day, and interactions. Accuracy for early pregnancy diagnosis (days 12–17) was assessed. CLBF:CLA ratio (14.3 ± 8.1 vs. 20.3 ± 10.7; 10.2 ± 10.4 vs. 17.8 ± 9.4; 1.3 ± 4.1 vs. 16.0 ± 8.2%), CLBF (0.41 ± 0.3 vs. 0.49 ± 0.3; 0.26 ± 0.3 vs. 0.45 ± 0.3; 0.06 ± 0.2 vs. 0.34 ± 0.2 cm2), and CLA (2.5 ± 1.1 vs. 2.3 ± 0.9; 1.9 ± 1.1 vs. 2.3 ± 1.1; 0.9 ± 1.2 vs. 2.0 ± 1.2 cm2) differed (P < 0.05) between pregnant and nonpregnant ewes at days 13, 14, and 16 after ovulation, respectively. The early diagnosis of nonpregnancy achieved accuracy values > 90% from day 15 on for CLBF and CLBF:CLA ratio, but not for CLA. At this day, CLBF and CLBF:CLA ratio were circa 50-fold greater in pregnant than in nonpregnant ewes (p < 0.0001). No ewe classified as in the 1st or 2nd quartiles for CL endpoints or P4 concentration was confirmed as pregnant at day 30. In summary, the diagnosis of nonpregnancy based on the objective evaluation of CLBF could be performed in sheep as early as at day 15 after ovulation. MenosThe study aimed to characterize morpho-functional changes in corpus luteum (CL) in pregnant and non-pregnant sheep, propose diagnostic criteria for non-pregnancy, and assess diagnostic accuracy around the expected luteolysis period. Crossbred ewes (n = 34) underwent estrous synchronization, divided into G1 (unmated, n = 20) and G2 (mated, n = 14). The day of ovulation was considered as day 0 (D0). Luteal dynamic was monitored from D0 to D17, with blood samples collected every 72 h for progesterone (P4). Pregnancy was definitively diagnosed at day 30. Analyses included CL area (CLA), CL blood flow (CLBF), CLBF:CLA ratio, and serum P4. Statistical models considered group, day, and interactions. Accuracy for early pregnancy diagnosis (days 12–17) was assessed. CLBF:CLA ratio (14.3 ± 8.1 vs. 20.3 ± 10.7; 10.2 ± 10.4 vs. 17.8 ± 9.4; 1.3 ± 4.1 vs. 16.0 ± 8.2%), CLBF (0.41 ± 0.3 vs. 0.49 ± 0.3; 0.26 ± 0.3 vs. 0.45 ± 0.3; 0.06 ± 0.2 vs. 0.34 ± 0.2 cm2), and CLA (2.5 ± 1.1 vs. 2.3 ± 0.9; 1.9 ± 1.1 vs. 2.3 ± 1.1; 0.9 ± 1.2 vs. 2.0 ± 1.2 cm2) differed (P < 0.05) between pregnant and nonpregnant ewes at days 13, 14, and 16 after ovulation, respectively. The early diagnosis of nonpregnancy achieved accuracy values > 90% from day 15 on for CLBF and CLBF:CLA ratio, but not for CLA. At this day, CLBF and CLBF:CLA ratio were circa 50-fold greater in pregnant than in nonpregnant ewes (p < 0.0001). No ewe classified as in the 1st or 2nd quartiles for CL endpoints or P4 concentration was confir... Mostrar Tudo |
|
Palavras-Chave: |
Luteal blood flow. |
|
Thesaurus Nal: |
Pregnancy; Sheep; Ultrasonography. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 02432naa a2200241 a 4500
001 2163793
005 2024-04-23
008 2024 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.smallrumres.2024.107211$2DOI
100 1 $aRIZZONI, L. B.
245 $aHow early can nonpregnant ewes be detected based on the blood flow of the corpus luteum?$h[electronic resource]
260 $c2024
520 $aThe study aimed to characterize morpho-functional changes in corpus luteum (CL) in pregnant and non-pregnant sheep, propose diagnostic criteria for non-pregnancy, and assess diagnostic accuracy around the expected luteolysis period. Crossbred ewes (n = 34) underwent estrous synchronization, divided into G1 (unmated, n = 20) and G2 (mated, n = 14). The day of ovulation was considered as day 0 (D0). Luteal dynamic was monitored from D0 to D17, with blood samples collected every 72 h for progesterone (P4). Pregnancy was definitively diagnosed at day 30. Analyses included CL area (CLA), CL blood flow (CLBF), CLBF:CLA ratio, and serum P4. Statistical models considered group, day, and interactions. Accuracy for early pregnancy diagnosis (days 12–17) was assessed. CLBF:CLA ratio (14.3 ± 8.1 vs. 20.3 ± 10.7; 10.2 ± 10.4 vs. 17.8 ± 9.4; 1.3 ± 4.1 vs. 16.0 ± 8.2%), CLBF (0.41 ± 0.3 vs. 0.49 ± 0.3; 0.26 ± 0.3 vs. 0.45 ± 0.3; 0.06 ± 0.2 vs. 0.34 ± 0.2 cm2), and CLA (2.5 ± 1.1 vs. 2.3 ± 0.9; 1.9 ± 1.1 vs. 2.3 ± 1.1; 0.9 ± 1.2 vs. 2.0 ± 1.2 cm2) differed (P < 0.05) between pregnant and nonpregnant ewes at days 13, 14, and 16 after ovulation, respectively. The early diagnosis of nonpregnancy achieved accuracy values > 90% from day 15 on for CLBF and CLBF:CLA ratio, but not for CLA. At this day, CLBF and CLBF:CLA ratio were circa 50-fold greater in pregnant than in nonpregnant ewes (p < 0.0001). No ewe classified as in the 1st or 2nd quartiles for CL endpoints or P4 concentration was confirmed as pregnant at day 30. In summary, the diagnosis of nonpregnancy based on the objective evaluation of CLBF could be performed in sheep as early as at day 15 after ovulation.
650 $aPregnancy
650 $aSheep
650 $aUltrasonography
653 $aLuteal blood flow
700 1 $aPALHÃO, M. P.
700 1 $aVIANA, J. H. M.
700 1 $aSOUZA, V. O.
700 1 $aNASCIMENTO NETO, J. da P.
700 1 $aNEVES, J. P.
773 $tSmall Ruminant Research$gv. 232, 107211, 2024.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Milho e Sorgo. |
|
Data corrente: |
23/11/2011 |
|
Data da última atualização: |
28/09/2017 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
A - 1 |
|
Autoria: |
RUIZ, J. C.; D'AFONSECA, V.; SILVA, A.; ALI, A.; PINTO, A. C.; SANTOS, A. R.; ROCHA, A. A. M. C.; LOPES, D. O.; DORELLA, F. A.; PACHECO, L. G. C.; COSTA, M. P.; TURK, M. Z.; SEYFFERT, N.; MORAES, P. M. R. O.; SOARES, S. C.; ALMEIDA, S. S.; CASTRO, T. L. P.; ABREU, V. A. C.; TROST, E.; BAUMBACH, J.; TAUCH, A.; SCHNEIDER, M. P. C.; McCULLOCH, J.; CERDEIRA, L. T.; RAMOS, R. T. J.; ZERLOTINI, A.; DOMINITINI, A.; RESENDE, D. M.; COSER, E. M.; OLIVEIRA, L. M.; PEDROSA, A. L.; VIEIRA, C. U.; GUIMARAES, C. T.; BARTHOLOMEU, D. C.; OLIVEIRA, D. M.; SANTOS, F. R.; RABELO, E. M.; LOBO, F. P.; FRANCO, G. R.; COSTA, A. F.; CASTRO, I. M.; DIAS, S. R. C.; FERRO, J. A.; ORTEGA, J. M.; PAIVA, L. V.; ALMEIDA, J. F.; GOULART, L. R.; FERRO, M. I. T.; CARNEIRO, N. P.; FALCÃO, P. R. K.; GRYNBERG, P.; TEIXEIRA, S. M. R.; BROMMONSCHENKEL, S.; OLIVEIRA, S. C.; MEYER, R.; MOORE, R. J.; MIYOSHI, A.; OLIVEIRA, G. C.; AZEVEDO, V. |
|
Afiliação: |
CLAUDIA TEIXEIRA GUIMARAES, CNPMS; NEWTON PORTILHO CARNEIRO, CNPMS. |
|
Título: |
Evidence for reductive genome evolution and lateral acquisition of virulence functions in two Corynebacterium pseudotuberculosis strains. |
|
Ano de publicação: |
2011 |
|
Fonte/Imprenta: |
Plos One, San Francisco, v. 6, n. 4, p. 1-16, 2011. |
|
DOI: |
10.1371/journal.pone.0018551 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Background: Corynebacterium pseudotuberculosis, a Gram-positive, facultative intracellular pathogen, is the etiologic agent of the disease known as caseous lymphadenitis (CL). CL mainly affects small ruminants, such as goats and sheep; it also causes infections in humans, though rarely. This species is distributed worldwide, but it has the most serious economic impact in Oceania, Africa and South America. Although C. pseudotuberculosis causes major health and productivity problems for livestock, little is known about the molecular basis of its pathogenicity. Methodology and Findings: We characterized two C. pseudotuberculosis genomes (Cp1002, isolated from goats; and CpC231, isolated from sheep). Analysis of the predicted genomes showed high similarity in genomic architecture, gene content and genetic order. When C. pseudotuberculosis was compared with other Corynebacterium species, it became evident that this pathogenic species has lost numerous genes, resulting in one of the smallest genomes in the genus. Other differences that could be part of the adaptation to pathogenicity include a lower GC content, of about 52%, and a reduced gene repertoire. The C. pseudotuberculosis genome also includes seven putative pathogenicity islands, which contain several classical virulence factors, including genes for fimbrial subunits, adhesion factors, iron uptake and secreted toxins. Additionally, all of the virulence factors in the islands have characteristics that indicate horizontal transfer. Conclusions: These particular genome characteristics of C. pseudotuberculosis, as well as its acquired virulence factors in pathogenicity islands, provide evidence of its lifestyle and of the pathogenicity pathways used by this pathogen in the infection process. All genomes cited in this study are available in the NCBI Genbank database (http://www.ncbi.nlm.nih.gov/genbank/) under accession numbers CP001809 and CP001829. MenosBackground: Corynebacterium pseudotuberculosis, a Gram-positive, facultative intracellular pathogen, is the etiologic agent of the disease known as caseous lymphadenitis (CL). CL mainly affects small ruminants, such as goats and sheep; it also causes infections in humans, though rarely. This species is distributed worldwide, but it has the most serious economic impact in Oceania, Africa and South America. Although C. pseudotuberculosis causes major health and productivity problems for livestock, little is known about the molecular basis of its pathogenicity. Methodology and Findings: We characterized two C. pseudotuberculosis genomes (Cp1002, isolated from goats; and CpC231, isolated from sheep). Analysis of the predicted genomes showed high similarity in genomic architecture, gene content and genetic order. When C. pseudotuberculosis was compared with other Corynebacterium species, it became evident that this pathogenic species has lost numerous genes, resulting in one of the smallest genomes in the genus. Other differences that could be part of the adaptation to pathogenicity include a lower GC content, of about 52%, and a reduced gene repertoire. The C. pseudotuberculosis genome also includes seven putative pathogenicity islands, which contain several classical virulence factors, including genes for fimbrial subunits, adhesion factors, iron uptake and secreted toxins. Additionally, all of the virulence factors in the islands have characteristics that indicate horizontal t... Mostrar Tudo |
|
Palavras-Chave: |
Análise de genoma; Genoma de C pseudotuberculosis; Ilhas de patogenicidade. |
|
Thesagro: |
Corynebacterium Pseudotuberculosis; Genética; Patógeno. |
|
Thesaurus NAL: |
Caseous lymphadenitis; Genome; Pathogenicity. |
|
Categoria do assunto: |
--
H Saúde e Patologia |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/47474/1/Evidence-reductive.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/53621/1/journal.pone.0018551.pdf
|
|
Marc: |
LEADER 04503naa a2200937 a 4500
001 1906799
005 2017-09-28
008 2011 bl uuuu u00u1 u #d
024 7 $a10.1371/journal.pone.0018551$2DOI
100 1 $aRUIZ, J. C.
245 $aEvidence for reductive genome evolution and lateral acquisition of virulence functions in two Corynebacterium pseudotuberculosis strains.$h[electronic resource]
260 $c2011
520 $aBackground: Corynebacterium pseudotuberculosis, a Gram-positive, facultative intracellular pathogen, is the etiologic agent of the disease known as caseous lymphadenitis (CL). CL mainly affects small ruminants, such as goats and sheep; it also causes infections in humans, though rarely. This species is distributed worldwide, but it has the most serious economic impact in Oceania, Africa and South America. Although C. pseudotuberculosis causes major health and productivity problems for livestock, little is known about the molecular basis of its pathogenicity. Methodology and Findings: We characterized two C. pseudotuberculosis genomes (Cp1002, isolated from goats; and CpC231, isolated from sheep). Analysis of the predicted genomes showed high similarity in genomic architecture, gene content and genetic order. When C. pseudotuberculosis was compared with other Corynebacterium species, it became evident that this pathogenic species has lost numerous genes, resulting in one of the smallest genomes in the genus. Other differences that could be part of the adaptation to pathogenicity include a lower GC content, of about 52%, and a reduced gene repertoire. The C. pseudotuberculosis genome also includes seven putative pathogenicity islands, which contain several classical virulence factors, including genes for fimbrial subunits, adhesion factors, iron uptake and secreted toxins. Additionally, all of the virulence factors in the islands have characteristics that indicate horizontal transfer. Conclusions: These particular genome characteristics of C. pseudotuberculosis, as well as its acquired virulence factors in pathogenicity islands, provide evidence of its lifestyle and of the pathogenicity pathways used by this pathogen in the infection process. All genomes cited in this study are available in the NCBI Genbank database (http://www.ncbi.nlm.nih.gov/genbank/) under accession numbers CP001809 and CP001829.
650 $aCaseous lymphadenitis
650 $aGenome
650 $aPathogenicity
650 $aCorynebacterium Pseudotuberculosis
650 $aGenética
650 $aPatógeno
653 $aAnálise de genoma
653 $aGenoma de C pseudotuberculosis
653 $aIlhas de patogenicidade
700 1 $aD'AFONSECA, V.
700 1 $aSILVA, A.
700 1 $aALI, A.
700 1 $aPINTO, A. C.
700 1 $aSANTOS, A. R.
700 1 $aROCHA, A. A. M. C.
700 1 $aLOPES, D. O.
700 1 $aDORELLA, F. A.
700 1 $aPACHECO, L. G. C.
700 1 $aCOSTA, M. P.
700 1 $aTURK, M. Z.
700 1 $aSEYFFERT, N.
700 1 $aMORAES, P. M. R. O.
700 1 $aSOARES, S. C.
700 1 $aALMEIDA, S. S.
700 1 $aCASTRO, T. L. P.
700 1 $aABREU, V. A. C.
700 1 $aTROST, E.
700 1 $aBAUMBACH, J.
700 1 $aTAUCH, A.
700 1 $aSCHNEIDER, M. P. C.
700 1 $aMcCULLOCH, J.
700 1 $aCERDEIRA, L. T.
700 1 $aRAMOS, R. T. J.
700 1 $aZERLOTINI, A.
700 1 $aDOMINITINI, A.
700 1 $aRESENDE, D. M.
700 1 $aCOSER, E. M.
700 1 $aOLIVEIRA, L. M.
700 1 $aPEDROSA, A. L.
700 1 $aVIEIRA, C. U.
700 1 $aGUIMARAES, C. T.
700 1 $aBARTHOLOMEU, D. C.
700 1 $aOLIVEIRA, D. M.
700 1 $aSANTOS, F. R.
700 1 $aRABELO, E. M.
700 1 $aLOBO, F. P.
700 1 $aFRANCO, G. R.
700 1 $aCOSTA, A. F.
700 1 $aCASTRO, I. M.
700 1 $aDIAS, S. R. C.
700 1 $aFERRO, J. A.
700 1 $aORTEGA, J. M.
700 1 $aPAIVA, L. V.
700 1 $aALMEIDA, J. F.
700 1 $aGOULART, L. R.
700 1 $aFERRO, M. I. T.
700 1 $aCARNEIRO, N. P.
700 1 $aFALCÃO, P. R. K.
700 1 $aGRYNBERG, P.
700 1 $aTEIXEIRA, S. M. R.
700 1 $aBROMMONSCHENKEL, S.
700 1 $aOLIVEIRA, S. C.
700 1 $aMEYER, R.
700 1 $aMOORE, R. J.
700 1 $aMIYOSHI, A.
700 1 $aOLIVEIRA, G. C.
700 1 $aAZEVEDO, V.
773 $tPlos One, San Francisco$gv. 6, n. 4, p. 1-16, 2011.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
