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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Corte. Para informações adicionais entre em contato com cnpgc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
04/12/2023 |
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Data da última atualização: |
04/12/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
DAMASCENO, A. G.; FERREIRA, M. T. M.; SOARES, I. C.; BARRIOS, S. C. L.; VALLE, C. B. do; TECHIO, V. H. |
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Afiliação: |
ANA GABRIELA DAMASCENO, UNIVERSIDADE FEDERAL DE LAVRAS; MARCO TÚLIO MENDES FERREIRA, UNIVERSIDADE FEDERAL DE LAVRAS; ISADORA CARDOSO SOARES, UNIVERSIDADE FEDERAL DE LAVRAS; SANZIO CARVALHO LIMA BARRIOS, CNPGC; CACILDA BORGES DO VALLE, CNPGC; VÂNIA HELENA TECHIO, UNIVERSIDADE FEDERAL DE LAVRAS. |
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Título: |
Physical mapping of ribosomal DNA sites and genome size in polyploid series of Urochloa humidicola (Rendle) Morrone & Zuloaga (Poaceae). |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Botany Letters, v. 170, n. 4, p. 634-643, 2023. |
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DOI: |
https://doi.org/10.1080/23818107.2023.2192274 |
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Idioma: |
Inglês |
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Notas: |
Published online 27 Mar 2023. |
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Conteúdo: |
ABSTRACT - Urochloa humidicola (Rendle) Morrone & Zuloaga is a species native to Africa that grows in tropical regions, especially in sites with temporary flooding. Despite its importance as forage crop, the karyotypic characterization of its cytotypes remains mainly unfinished, and its polyploid origin is unknown. Therefore, cytogenetic studies can provide information about the evolutionary history of species and to characterize the core and reserve collection of the germplasm banks. We performed the karyotype analyses, mapped ribosomal sites, and estimated genome size, confirming three ploidy levels (6×, 7×, and 9×) and one aneuploid accession H031 (2n = 6× = 36 + 1). Karyotypes are symmetrical and the number of 35S and 5S rDNA sites varied between the cytotypes. However, all sites were at terminal and interstitial positions in the chromosomes, respectively, except one chromosome of the aneuploid accession with interstitial colocalization. The polymorphism of the number and distribution of rDNA sites in U. humidicola was confirmed by (i) the variation between and within plants and in metaphases of the same meristem in hexaploids and heptaploids; (ii) the occurrence of rDNA sites in hemizygosis in all accessions, except heptaploids; (iii) the size heteromorphism in some ribosomal sites of homologous chromosomes. The karyotype data and the mapping of ribosomal sites corroborate the proposals of allopolyploidy in U. humidicola and the hybrid origin to the H031 accession. |
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Thesagro: |
Banco de Germoplasma; Brachiaria; Capim Urochloa. |
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Thesaurus Nal: |
Aneuploidy; Cytotypes; Flow cytometry; Forage crops; Ribosomal DNA. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02443naa a2200301 a 4500
001 2159110
005 2023-12-04
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1080/23818107.2023.2192274$2DOI
100 1 $aDAMASCENO, A. G.
245 $aPhysical mapping of ribosomal DNA sites and genome size in polyploid series of Urochloa humidicola (Rendle) Morrone & Zuloaga (Poaceae).$h[electronic resource]
260 $c2023
500 $aPublished online 27 Mar 2023.
520 $aABSTRACT - Urochloa humidicola (Rendle) Morrone & Zuloaga is a species native to Africa that grows in tropical regions, especially in sites with temporary flooding. Despite its importance as forage crop, the karyotypic characterization of its cytotypes remains mainly unfinished, and its polyploid origin is unknown. Therefore, cytogenetic studies can provide information about the evolutionary history of species and to characterize the core and reserve collection of the germplasm banks. We performed the karyotype analyses, mapped ribosomal sites, and estimated genome size, confirming three ploidy levels (6×, 7×, and 9×) and one aneuploid accession H031 (2n = 6× = 36 + 1). Karyotypes are symmetrical and the number of 35S and 5S rDNA sites varied between the cytotypes. However, all sites were at terminal and interstitial positions in the chromosomes, respectively, except one chromosome of the aneuploid accession with interstitial colocalization. The polymorphism of the number and distribution of rDNA sites in U. humidicola was confirmed by (i) the variation between and within plants and in metaphases of the same meristem in hexaploids and heptaploids; (ii) the occurrence of rDNA sites in hemizygosis in all accessions, except heptaploids; (iii) the size heteromorphism in some ribosomal sites of homologous chromosomes. The karyotype data and the mapping of ribosomal sites corroborate the proposals of allopolyploidy in U. humidicola and the hybrid origin to the H031 accession.
650 $aAneuploidy
650 $aCytotypes
650 $aFlow cytometry
650 $aForage crops
650 $aRibosomal DNA
650 $aBanco de Germoplasma
650 $aBrachiaria
650 $aCapim Urochloa
700 1 $aFERREIRA, M. T. M.
700 1 $aSOARES, I. C.
700 1 $aBARRIOS, S. C. L.
700 1 $aVALLE, C. B. do
700 1 $aTECHIO, V. H.
773 $tBotany Letters$gv. 170, n. 4, p. 634-643, 2023.
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Embrapa Gado de Corte (CNPGC) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
24/08/2023 |
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Data da última atualização: |
31/10/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 3 |
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Autoria: |
OLIVEIRA, C. S.; ROSA, P. M. da S.; CAMARGO, A. J. dos R.; FERES, L. F.; SARAIVA, N. Z.; OLIVEIRA, L. Z.; CAMARGO, L. S. de A. |
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Afiliação: |
CLARA SLADE OLIVEIRA, CNPGL; PAOLA MARIA DA SILVA ROSA; AGOSTINHO JORGE DOS REIS CAMARGO, Pesagro; LUIZ FERNANDO FERES, Universidade José do Rosário Vellano-Unifenas; NAIARA ZOCCAL SARAIVA, CNPGL; LETICIA ZOCCOLARO OLIVEIRA, Universidade Federal de Minas Gerais; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL. |
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Título: |
Outstanding Gir oocyte donors: how does individual factor affect in vitro embryo production efficiency? |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Animal Science Journal, v. 94, e13862, 2023. |
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DOI: |
https://doi.org/10.1111/asj.13862 |
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Idioma: |
Inglês |
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Conteúdo: |
The oocyte donor plays a pivotal role in bovine in vitro embryo production (IVP) success. The individual factor affects blastocyst/oocyte ratio and determine the existence of outstanding performing animals. The aim of this study was to assess the extent of individual factor effect to IVP efficiency, in a population of Gir oocyte donors. Extreme (high or low IVP efficiency based on blastocyst/oocyte ratio) animals were selected out of a population of 250 oocyte donors (1,734 observations) to form high (>0.48, n = 40), average (0.17-0.48, n = 168), and low (<0.17, n = 42) efficiency donor groups. Cumulus-oocyte complex indicators (total number, IVF-grade number, and IVF-grade/total ratio) were lower (p < 0.05) in high efficiency donors. The number of blastocysts per OPU was analyzed for highest performing bull, and an increase (p < 0.05) in high efficiency donors and a decrease (p < 0.05) in low efficiency donors were noticed, compared to average efficiency donors. The number of pregnancies obtained per OPU was affected (p = 0.017) by donor?s efficiency (low: 0:600:09, average: 1:170:07, high: 2:570:26), being 4.3-fold higher in high than in low efficiency donors. We conclude that producing embryos from high efficiency blastocyst/oocyte ratio donors increases blastocyst and pregnancy numbers by OPU, being an important indicator for donor selection in IVP programs |
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Palavras-Chave: |
Eficiência da PIV; Fertilização in vitro. |
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Thesagro: |
Gado Gir; Reprodução Animal. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02181naa a2200253 a 4500
001 2156100
005 2023-10-31
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1111/asj.13862$2DOI
100 1 $aOLIVEIRA, C. S.
245 $aOutstanding Gir oocyte donors$bhow does individual factor affect in vitro embryo production efficiency?$h[electronic resource]
260 $c2023
520 $aThe oocyte donor plays a pivotal role in bovine in vitro embryo production (IVP) success. The individual factor affects blastocyst/oocyte ratio and determine the existence of outstanding performing animals. The aim of this study was to assess the extent of individual factor effect to IVP efficiency, in a population of Gir oocyte donors. Extreme (high or low IVP efficiency based on blastocyst/oocyte ratio) animals were selected out of a population of 250 oocyte donors (1,734 observations) to form high (>0.48, n = 40), average (0.17-0.48, n = 168), and low (<0.17, n = 42) efficiency donor groups. Cumulus-oocyte complex indicators (total number, IVF-grade number, and IVF-grade/total ratio) were lower (p < 0.05) in high efficiency donors. The number of blastocysts per OPU was analyzed for highest performing bull, and an increase (p < 0.05) in high efficiency donors and a decrease (p < 0.05) in low efficiency donors were noticed, compared to average efficiency donors. The number of pregnancies obtained per OPU was affected (p = 0.017) by donor?s efficiency (low: 0:600:09, average: 1:170:07, high: 2:570:26), being 4.3-fold higher in high than in low efficiency donors. We conclude that producing embryos from high efficiency blastocyst/oocyte ratio donors increases blastocyst and pregnancy numbers by OPU, being an important indicator for donor selection in IVP programs
650 $aGado Gir
650 $aReprodução Animal
653 $aEficiência da PIV
653 $aFertilização in vitro
700 1 $aROSA, P. M. da S.
700 1 $aCAMARGO, A. J. dos R.
700 1 $aFERES, L. F.
700 1 $aSARAIVA, N. Z.
700 1 $aOLIVEIRA, L. Z.
700 1 $aCAMARGO, L. S. de A.
773 $tAnimal Science Journal$gv. 94, e13862, 2023.
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