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 | Acesso ao texto completo restrito à biblioteca da Embrapa Café. Para informações adicionais entre em contato com biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
27/03/2023 |
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Data da última atualização: |
12/04/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ANGELO, P. C. da S.; PEREIRA, L. F. P.; SERA, G. H.; SETA, T. |
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Afiliação: |
PAULA CRISTINA DA SILVA ANGELO, CNPCa; L. F. P. PEREIRA, IDR - PARANÁ; G. H. SERA, IDR - PARANÁ; T. SERA, IDR - PARANÁ. |
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Título: |
Coffee microspore cultivation to attain doubled-haploid plantlets. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Acta Horticulturae, v. 31, n. 1359, p. 123-130, 2023. |
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DOI: |
10.17660/ActaHortic.2023.1359.14 |
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Idioma: |
Inglês |
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Notas: |
Apresentado no INTERNATIONAL HORTICULTURAL CONGRESS, 31., 2022, Angers, França. |
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Conteúdo: |
Introgression of disease resistance from related Coffea species to C. arabica has been done by inter-specific hybridization. C. arabica (tetraploid) × C. racemosa (diploid) hybrids were obtained at the IDR-Paraná, Brazil. Some of the hybrids display impaired self-pollination, despite backcrossed to C. arabica. As an attempt to regenerate stable doubled-haploid plants, anthers and isolated microspores have been cultivated in vitro. It was considered that chromosomes captured in a microspore following meiosis could acquire homo and/or homeologous stability faster in vitro than in vivo. A C. arabica progeny was taken as control. Flowers were collected at the IDR campus in Londrina, when young microspores were uninucleate, and treated with 8% active chlorine. Microspores were extracted in 90 mM mannitol using a food mixer set to function for a few seconds, washed and centrifuged at 100× g twice, and cultivated in modified N6 liquid medium (105 cells mL-1 in 35 mm diameter plates). Anthers were excised using scalpels and cultivated in solid medium. Explants were kept for six months of continuous cultivation on that induction medium containing 6.5 mg L-1 auxins, 1.0 mg L-1 cytokinins and 0.5 mg L-1 gibberellin (GA3), at 27°C under dark. Microspores produced embryo-like structures or microcalli in very low frequencies (0.3 per plate). Anthers, on the other hand, produced embryogenic calli. Colonization by an invariant morphotype of fungus took away 70% of Arabica coffee control anthers and 5% of the asseptic anthers produced friable calli, some embryogenic but arrested when compared with calli produced by the inter-specific hybrid anthers. Colonization by an invariant morphotype of bacteria took away 25% of hybrid anthers and 8% of produced embryogenic tissue, with globular embryos simultaneously multiplying and maturing upon transfer to N6 medium. Embryo conversion and photomorphogenesis under light on auxin/cytokinin ratio = 2 plus GA3 are going on, 1.2 year after inoculation. MenosIntrogression of disease resistance from related Coffea species to C. arabica has been done by inter-specific hybridization. C. arabica (tetraploid) × C. racemosa (diploid) hybrids were obtained at the IDR-Paraná, Brazil. Some of the hybrids display impaired self-pollination, despite backcrossed to C. arabica. As an attempt to regenerate stable doubled-haploid plants, anthers and isolated microspores have been cultivated in vitro. It was considered that chromosomes captured in a microspore following meiosis could acquire homo and/or homeologous stability faster in vitro than in vivo. A C. arabica progeny was taken as control. Flowers were collected at the IDR campus in Londrina, when young microspores were uninucleate, and treated with 8% active chlorine. Microspores were extracted in 90 mM mannitol using a food mixer set to function for a few seconds, washed and centrifuged at 100× g twice, and cultivated in modified N6 liquid medium (105 cells mL-1 in 35 mm diameter plates). Anthers were excised using scalpels and cultivated in solid medium. Explants were kept for six months of continuous cultivation on that induction medium containing 6.5 mg L-1 auxins, 1.0 mg L-1 cytokinins and 0.5 mg L-1 gibberellin (GA3), at 27°C under dark. Microspores produced embryo-like structures or microcalli in very low frequencies (0.3 per plate). Anthers, on the other hand, produced embryogenic calli. Colonization by an invariant morphotype of fungus took away 70% of Arabica coffee control ant... Mostrar Tudo |
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Thesagro: |
Embriogénese; Pólen; Rubiaceae. |
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Thesaurus Nal: |
Embryogenesis; Pollen. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02752naa a2200241 a 4500
001 2152776
005 2023-04-12
008 2023 bl uuuu u00u1 u #d
024 7 $a10.17660/ActaHortic.2023.1359.14$2DOI
100 1 $aANGELO, P. C. da S.
245 $aCoffee microspore cultivation to attain doubled-haploid plantlets.$h[electronic resource]
260 $c2023
500 $aApresentado no INTERNATIONAL HORTICULTURAL CONGRESS, 31., 2022, Angers, França.
520 $aIntrogression of disease resistance from related Coffea species to C. arabica has been done by inter-specific hybridization. C. arabica (tetraploid) × C. racemosa (diploid) hybrids were obtained at the IDR-Paraná, Brazil. Some of the hybrids display impaired self-pollination, despite backcrossed to C. arabica. As an attempt to regenerate stable doubled-haploid plants, anthers and isolated microspores have been cultivated in vitro. It was considered that chromosomes captured in a microspore following meiosis could acquire homo and/or homeologous stability faster in vitro than in vivo. A C. arabica progeny was taken as control. Flowers were collected at the IDR campus in Londrina, when young microspores were uninucleate, and treated with 8% active chlorine. Microspores were extracted in 90 mM mannitol using a food mixer set to function for a few seconds, washed and centrifuged at 100× g twice, and cultivated in modified N6 liquid medium (105 cells mL-1 in 35 mm diameter plates). Anthers were excised using scalpels and cultivated in solid medium. Explants were kept for six months of continuous cultivation on that induction medium containing 6.5 mg L-1 auxins, 1.0 mg L-1 cytokinins and 0.5 mg L-1 gibberellin (GA3), at 27°C under dark. Microspores produced embryo-like structures or microcalli in very low frequencies (0.3 per plate). Anthers, on the other hand, produced embryogenic calli. Colonization by an invariant morphotype of fungus took away 70% of Arabica coffee control anthers and 5% of the asseptic anthers produced friable calli, some embryogenic but arrested when compared with calli produced by the inter-specific hybrid anthers. Colonization by an invariant morphotype of bacteria took away 25% of hybrid anthers and 8% of produced embryogenic tissue, with globular embryos simultaneously multiplying and maturing upon transfer to N6 medium. Embryo conversion and photomorphogenesis under light on auxin/cytokinin ratio = 2 plus GA3 are going on, 1.2 year after inoculation.
650 $aEmbryogenesis
650 $aPollen
650 $aEmbriogénese
650 $aPólen
650 $aRubiaceae
700 1 $aPEREIRA, L. F. P.
700 1 $aSERA, G. H.
700 1 $aSETA, T.
773 $tActa Horticulturae$gv. 31, n. 1359, p. 123-130, 2023.
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Registro original: |
Embrapa Café (CNPCa) |
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| Registros recuperados : 4 | |
| 1. |  | SANTOS, G. R.; SANTOS, L. C. de S.; ALMEIDA, R. N. de; PEREIRA, H. dos S.; TRINDADE, I. de M.; PRETTI, I. R.; BARBOSA, D. H. S. G.; AMORIM, E. P.; BERILLI, S. da S.; BERILLI, A. P. C. G. Simultaneous gains for negatively correlated traits of interest in plantain. Pesquisa Agropecuária Brasileira, v. 59, e03458, 2024. Título em português: Ganhos simultâneos em caracteres de interesse negativamente correlacionados em banana-da-terra.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 2. | | FERNANDES, G. R.; BARBOSA, A. E. A. D.; ALMEIDA, R. N.; CASTRO, F. F. dos S.; PONTE, M. de C. P. da; FARIA-JUNIOR, C.; MÜLLER, F. M. P.; VIANA, A. A. B.; GRATTAPAGLIA, D.; FRANCO, O. L.; ALENCAR, S. A.; DIAS, S. C. Genomic comparison among lethal invasive strains of Streptococcus pyogenes Serotype M1. Frontiers in Microbiology, v. 8, 1993, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 3. | | OLIVEIRA, T. R. A. de; GRAVINA, G. do A.; ROCHA, M. de M.; ALCÂNTARA NETO, F. de; FERREIRA, G. H. F. de; ALMEIDA, R. N. de; GONÇALVES, G. R.; MORAES, R.; ROCHA, R. S.; CHAGAS, J. T. B.; SANT'ANNA, C. Q. da S. S. de. Ganho de seleção no melhoramento genético de feijão-caupi. In: ENCONTRO LATINO AMERICANO DE INICIAÇÃO CIENTÍFICA, 22.; ENCONTRO LATINO AMERICANO DE PÓS-GRADUAÇÃO, 28.; ENCONTRO DE INICIAÇÃO À DOCÊNCIA, 8., 2018, São José dos Campos, SP. Patrimônio e cultura desafio da ciência frente a identidades plurais: anais. São José dos Campos, SP: Universidade do Vale do Paraíba, 2018. 6 p.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio-Norte. |
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| 4. | | OLIVEIRA, T. R. A.; SANT'ANNA, C. Q. S. S.; GRAVINA, G. A.; ROCHA, M. de M.; ALCÂNTARA NETO, F.; CRUZ, D. P.; OLIVEIRA, G. H. F.; GRAVINA, L. M.; ROCHA, R. S.; PEREIRA, I. M.; JAEGGI, M. E. P. C.; CHAGAS, J. T. B. C.; ALMEIDA, R. N. Selección de linajes de frijol caupí (Vigna unguiculata L. Walp.) a través del análisis multivariado. In: CONGRESO ARGENTINO DE HORTICULTURA, 40., 2018, Córdoba. Nuevos escénarios e innovación para cadenas de valor sustentables: resumos. Buenos Aires: ASAHO, 2018. p. 260.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio-Norte. |
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| Registros recuperados : 4 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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