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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
07/03/2023 |
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Data da última atualização: |
07/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SCHWAB, S.; HIRATA, E. S.; AMARAL, J. C. A.; SILVA, C. G. N.; FERREIRA, J. P.; SILVA, L. V. da; ROUWS, J. R. C.; ROUWS, L. F. M.; BALDANI, J. I.; REIS, V. M. |
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Afiliação: |
STEFAN SCHWAB, CNPAB; BASF S.A; J. C. A. AMARAL, UFRRJ; C. G. N. DA SILVA, BOLSISTA EMBRAPA AGROBIOLOGIA; J. P. FERREIRA, SOLUBIO; L. V. DA SILVA, BOLSISTA EMBRAPA AGROBIOLOGIA; JANAINA RIBEIRO COSTA ROUWS, CNPAB; LUC FELICIANUS MARIE ROUWS, CNPAB; JOSE IVO BALDANI, CNPAB; VERONICA MASSENA REIS, CNPAB. |
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Título: |
Quantifying and visualizing Nitrospirillum amazonense strain CBAmC in sugarcane after using different inoculation methods. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Plant and Soil , Published: 22 February 2023. |
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ISSN: |
1573-5036 |
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Idioma: |
Inglês |
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Conteúdo: |
Nitrospirillum amazonense strain CBAmC is a nitrogen-fixing bacterium that has been used for sugarcane inoculation. Despite its beneficial effects, however, little is known about the establishment and colonization of this microorganism in its inoculated host plant. Furthermore, alternative inoculation methods need to be tested in the sugarcane crop. The objective of the present work was to quantify and visualize CBAmC in sugarcane cultivar RB867515 after using three inoculation methods. Methods: Utilizing one node as propagation material, three bacterial inoculation methods were tested under greenhouse conditions. For up to 60 days after inoculation (DAI), qPCR was used to quantify the bacterial loads in the plant or substrate while FISH-CLSM was used to confirm the establishment and colonization profile of CBAmC in different plant tissues. Results: FISH-CLSM indicated that the CBAmC strain could establish itself on different tissues of sugarcane, especially roots. qPCR revealed variable bacterial loads in the plants inoculated according to the three methods tested. In addition, little or no bacterial loads were detected in the substrate a few days after inoculation, regardless of the method used. Conclusions: N. amazonense CBAmC proves to be a bacterium that preferentially associates with the surface of the root tissues of sugarcane propagated by one node, instead of colonizing internal plant tissues or remaining in the soil. The inoculation method consisting of immersion of the mini-setts in bacterial suspension proved to be more efficient than the inoculation methods of applying the suspension onto the post-sprouting substrate or to the lowest lea MenosNitrospirillum amazonense strain CBAmC is a nitrogen-fixing bacterium that has been used for sugarcane inoculation. Despite its beneficial effects, however, little is known about the establishment and colonization of this microorganism in its inoculated host plant. Furthermore, alternative inoculation methods need to be tested in the sugarcane crop. The objective of the present work was to quantify and visualize CBAmC in sugarcane cultivar RB867515 after using three inoculation methods. Methods: Utilizing one node as propagation material, three bacterial inoculation methods were tested under greenhouse conditions. For up to 60 days after inoculation (DAI), qPCR was used to quantify the bacterial loads in the plant or substrate while FISH-CLSM was used to confirm the establishment and colonization profile of CBAmC in different plant tissues. Results: FISH-CLSM indicated that the CBAmC strain could establish itself on different tissues of sugarcane, especially roots. qPCR revealed variable bacterial loads in the plants inoculated according to the three methods tested. In addition, little or no bacterial loads were detected in the substrate a few days after inoculation, regardless of the method used. Conclusions: N. amazonense CBAmC proves to be a bacterium that preferentially associates with the surface of the root tissues of sugarcane propagated by one node, instead of colonizing internal plant tissues or remaining in the soil. The inoculation method consisting of immersion o... Mostrar Tudo |
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Palavras-Chave: |
Nitrospirillum amazonense; Promoting plant growth. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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| 1. |  | MAGALHÃES, J. A.; LOPES, E. A.; RODRIGUES, B. H. N.; ARAÚJO NETTO, R. B. de; COSTA, N. de L.; LOPES NETO, L.; BEZERRA, E. E. A. Eficiência técnica de um sistema de produção com gado mestiço para as bacias leiteiras dos Estados do Piauí e do Maranhão. Agropecuária Catarinense, v. 16, n. 3, p. 30-32, nov. 2003.| Biblioteca(s): Embrapa Meio-Norte. |
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