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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
27/01/2023 |
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Data da última atualização: |
24/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MACHADO-ROSA, T. A.; BARBOSA, E. T.; CÔRTES, M. V. de C. B.; LOBO JUNIOR, M. |
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Afiliação: |
TARIANE A. MACHADO-ROSA, UNIVERSIDADE FEDERAL DE GOIÁS; ELDER TADEU BARBOSA, CNPAF; MARCIO VINICIUS DE C BARROS CORTES, CNPAF; MURILLO LOBO JUNIOR, CNPAF. |
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Título: |
Microtiter method for quantitative assay of IAA from fungal isolates, demonstrated with Trichoderma. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Rhizosphere, v. 25, 100666, 2023. |
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ISSN: |
2452-2198 |
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DOI: |
https://doi.org/10.1016/j.rhisph.2023.100666 |
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Idioma: |
Inglês |
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Conteúdo: |
A quantitative and accurate method is needed to estimate indole-3-acetic acid (IAA) production by Trichoderma to compare strains and allow the repeatability of experiments in plant-growth promotion studies. Suspensions of 1.0 × 106 conidia. mL-1 of 98 Brazilian strains were grown in potato + dextrose broth in the absence or presence of L-tryptophan (100 mg L-1) in 1 mL microtubes. After 12,000 rpm spinning for 15 min, 150 μL of the super-natant were added to 100 μL of Salkowski reagent with 30 min incubation at room temperature in the dark, with IAA yield spectrophotometrically estimated at 540 nm. Isolates BRM-29474 and BRM-29574 that outperformed in the IAA production increased tomato root and shoot mass and number of root tips in a greenhouse test, endorsing their potential for plant growth promotion. This adjustment allows a quantitative method to estimate an IAA production by Trichoderma spp., which is more informative than the standard qualitative method. |
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Palavras-Chave: |
Biocontrol; Plant growth promotion; Stepwise screening. |
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Thesagro: |
Estimulante de Crescimento Vegetal; Fungo; Trichoderma. |
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Thesaurus Nal: |
Auxins; Plant growth; Root exudates. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 01851naa a2200289 a 4500
001 2151274
005 2023-03-24
008 2023 bl uuuu u00u1 u #d
022 $a2452-2198
024 7 $ahttps://doi.org/10.1016/j.rhisph.2023.100666$2DOI
100 1 $aMACHADO-ROSA, T. A.
245 $aMicrotiter method for quantitative assay of IAA from fungal isolates, demonstrated with Trichoderma.$h[electronic resource]
260 $c2023
520 $aA quantitative and accurate method is needed to estimate indole-3-acetic acid (IAA) production by Trichoderma to compare strains and allow the repeatability of experiments in plant-growth promotion studies. Suspensions of 1.0 × 106 conidia. mL-1 of 98 Brazilian strains were grown in potato + dextrose broth in the absence or presence of L-tryptophan (100 mg L-1) in 1 mL microtubes. After 12,000 rpm spinning for 15 min, 150 μL of the super-natant were added to 100 μL of Salkowski reagent with 30 min incubation at room temperature in the dark, with IAA yield spectrophotometrically estimated at 540 nm. Isolates BRM-29474 and BRM-29574 that outperformed in the IAA production increased tomato root and shoot mass and number of root tips in a greenhouse test, endorsing their potential for plant growth promotion. This adjustment allows a quantitative method to estimate an IAA production by Trichoderma spp., which is more informative than the standard qualitative method.
650 $aAuxins
650 $aPlant growth
650 $aRoot exudates
650 $aEstimulante de Crescimento Vegetal
650 $aFungo
650 $aTrichoderma
653 $aBiocontrol
653 $aPlant growth promotion
653 $aStepwise screening
700 1 $aBARBOSA, E. T.
700 1 $aCÔRTES, M. V. de C. B.
700 1 $aLOBO JUNIOR, M.
773 $tRhizosphere$gv. 25, 100666, 2023.
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Embrapa Arroz e Feijão (CNPAF) |
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| 1. |  | PICCOLI, M. L.; BRACINNI NETO, J.; CARDOSO, F. F.; SARGOLZAEI, M.; SCHENKEL, F. S. Accuracy of genotype imputation with different low density panels in Braford and Hereford cattle. In: WORLD CONGRESS ON GENETICS APPLIED TO LIVESTOCK PRODUCTION, 10., 2014, Vancouver, British Columbia, Canada. Proceedings... Champaign: ASAS, 2014. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sul. |
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| 4. | | PICCOLI, M. L.; BRITO, L. F.; BRACCINI, J.; BRITO, F. V.; CARDOSO, F. F.; COBUCI, J. A.; SARGOLZAEI, M.; SCHENKEL, F. S. A comprehensive comparison between single- and two-step GBLUP methods in a simulated beef cattle population. Canadian Journal of Animal Science, v. 98, n. 3, p. 565-575, Sept. 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Pecuária Sul. |
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| Registros recuperados : 4 | |
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