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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
08/04/2022 |
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Data da última atualização: |
08/04/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA, M. E. F.; ARRAIS, A. M.; MELLO, M. R. B. de; VERGANI, G. B.; FIGUEIRA, L. M.; ESTEVES, S. N.; PEREIRA, V. S. d. A.; GARCIA, A. R.; BARTLEWSKI, P. M.; FONSECA, J. F. da. |
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Afiliação: |
MARIA EMILIA FRANCO OLIVEIRA; ALINE MATOS ARRAIS; MARCO ROBERTO BOURG DE MELLO; GABRIEL BRUN VERGANI; LUCAS MACHADO FIGUEIRA; SÉRGIO NOVITA ESTEVES; VERÔNICA SCHINAIDER DO AMARAL PEREIRA; ALEXANDRE ROSSETTO GARCIA; PAWEL MIECZYSLAW BARTLEWSKI; JEFERSON FERREIRA DA FONSECA, CNPC. |
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Título: |
Study of the factors affecting embryo yields and quality in superovulated Morada Nova ewes that underwent non-surgical uterine flushing. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Reproduction in Domestic Animals, v. 57, n. 4, p. 393-401, Jan. 2022. |
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DOI: |
10.1111/rda.14077 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The present study compared the outcomes of in vivo embryo production in Morada Nova ewes subjected to either 9-day (G-9SOV , n = 21) or 12-day (G-12SOV , n = 21) progesterone (P4 )-based estruses synchronization protocol coupled with superovulatory treatment with decreasing doses of porcine follicle-stimulating hormone (133 mg of pFSH given over 3 days). Non-surgical embryo recovery (NSER) was performed 6-7 days after the onset of oestrus. Total antral follicle count doubled from the first to the sixth pFSH dose in both groups (p < .05). Oestrus responses did not vary between the two groups of animals (95.2%). Corpora lutea (CL) were detected in 85.0% and 60.0% of ewes that previously manifested oestrus behaviour in G-9SOV and G-12SOV respectively. NSER was successfully completed in 86.2% of ewes that had CL (p > .05). The mean number of CL per ewe/successfully flushed donor ewe was greater (p < .05) in G-12SOV (12.3 ± 1.7/12.1 ± 1.9) than in G-9SOV (7.9 ± 1.4/8.2 ± 1.6). Mean numbers of retrieved blastocysts and viable embryos were greater (p > .05) in G-12SOV (5.8 ± 1.9 and 3.7 ± 1.7) than G-9SOV (3.5 ± 1.1 and 0.8 ± 0.3 respectively). The total follicle count (all follicles ?2 mm in diameter) at the sixth pFSH dose (at P4 -device removal) was positively correlated (p < .05) with the number of CL (r = .95) and viable embryos (r = .91) in G-12SOV . The ewes with ?10 Cl (48% of all flushed donors) yielded 80.5% of viable embryos. In summary: (a) Morada Nova ewes from G-12SOV group had better superovulatory responses compared with G-9SOV group; (b) total follicle count at the last pFSH dose was a good predictor of superovulatory responses only in the ewes primed with P4 for 12 days; and (c) animals with ?10 ovulations are main contributors to viable embryo production in Morada Nova ewes. MenosAbstract: The present study compared the outcomes of in vivo embryo production in Morada Nova ewes subjected to either 9-day (G-9SOV , n = 21) or 12-day (G-12SOV , n = 21) progesterone (P4 )-based estruses synchronization protocol coupled with superovulatory treatment with decreasing doses of porcine follicle-stimulating hormone (133 mg of pFSH given over 3 days). Non-surgical embryo recovery (NSER) was performed 6-7 days after the onset of oestrus. Total antral follicle count doubled from the first to the sixth pFSH dose in both groups (p < .05). Oestrus responses did not vary between the two groups of animals (95.2%). Corpora lutea (CL) were detected in 85.0% and 60.0% of ewes that previously manifested oestrus behaviour in G-9SOV and G-12SOV respectively. NSER was successfully completed in 86.2% of ewes that had CL (p > .05). The mean number of CL per ewe/successfully flushed donor ewe was greater (p < .05) in G-12SOV (12.3 ± 1.7/12.1 ± 1.9) than in G-9SOV (7.9 ± 1.4/8.2 ± 1.6). Mean numbers of retrieved blastocysts and viable embryos were greater (p > .05) in G-12SOV (5.8 ± 1.9 and 3.7 ± 1.7) than G-9SOV (3.5 ± 1.1 and 0.8 ± 0.3 respectively). The total follicle count (all follicles ?2 mm in diameter) at the sixth pFSH dose (at P4 -device removal) was positively correlated (p < .05) with the number of CL (r = .95) and viable embryos (r = .91) in G-12SOV . The ewes with ?10 Cl (48% of all flushed donors) yielded 80.5% of viable embryos. In summary: (a) Morada Nova ewes fr... Mostrar Tudo |
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Palavras-Chave: |
Follicle Stimulating Hormone; In vivo embryo production; Naturalized sheep breed; Non-surgical embryo recovery; NSER; Ovarian Follicle. |
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Thesaurus Nal: |
Corpus luteum; Estrus synchronization; Sheep; Superovulation. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02983naa a2200361 a 4500
001 2142007
005 2022-04-08
008 2022 bl uuuu u00u1 u #d
024 7 $a10.1111/rda.14077$2DOI
100 1 $aOLIVEIRA, M. E. F.
245 $aStudy of the factors affecting embryo yields and quality in superovulated Morada Nova ewes that underwent non-surgical uterine flushing.$h[electronic resource]
260 $c2022
520 $aAbstract: The present study compared the outcomes of in vivo embryo production in Morada Nova ewes subjected to either 9-day (G-9SOV , n = 21) or 12-day (G-12SOV , n = 21) progesterone (P4 )-based estruses synchronization protocol coupled with superovulatory treatment with decreasing doses of porcine follicle-stimulating hormone (133 mg of pFSH given over 3 days). Non-surgical embryo recovery (NSER) was performed 6-7 days after the onset of oestrus. Total antral follicle count doubled from the first to the sixth pFSH dose in both groups (p < .05). Oestrus responses did not vary between the two groups of animals (95.2%). Corpora lutea (CL) were detected in 85.0% and 60.0% of ewes that previously manifested oestrus behaviour in G-9SOV and G-12SOV respectively. NSER was successfully completed in 86.2% of ewes that had CL (p > .05). The mean number of CL per ewe/successfully flushed donor ewe was greater (p < .05) in G-12SOV (12.3 ± 1.7/12.1 ± 1.9) than in G-9SOV (7.9 ± 1.4/8.2 ± 1.6). Mean numbers of retrieved blastocysts and viable embryos were greater (p > .05) in G-12SOV (5.8 ± 1.9 and 3.7 ± 1.7) than G-9SOV (3.5 ± 1.1 and 0.8 ± 0.3 respectively). The total follicle count (all follicles ?2 mm in diameter) at the sixth pFSH dose (at P4 -device removal) was positively correlated (p < .05) with the number of CL (r = .95) and viable embryos (r = .91) in G-12SOV . The ewes with ?10 Cl (48% of all flushed donors) yielded 80.5% of viable embryos. In summary: (a) Morada Nova ewes from G-12SOV group had better superovulatory responses compared with G-9SOV group; (b) total follicle count at the last pFSH dose was a good predictor of superovulatory responses only in the ewes primed with P4 for 12 days; and (c) animals with ?10 ovulations are main contributors to viable embryo production in Morada Nova ewes.
650 $aCorpus luteum
650 $aEstrus synchronization
650 $aSheep
650 $aSuperovulation
653 $aFollicle Stimulating Hormone
653 $aIn vivo embryo production
653 $aNaturalized sheep breed
653 $aNon-surgical embryo recovery
653 $aNSER
653 $aOvarian Follicle
700 1 $aARRAIS, A. M.
700 1 $aMELLO, M. R. B. de
700 1 $aVERGANI, G. B.
700 1 $aFIGUEIRA, L. M.
700 1 $aESTEVES, S. N.
700 1 $aPEREIRA, V. S. d. A.
700 1 $aGARCIA, A. R.
700 1 $aBARTLEWSKI, P. M.
700 1 $aFONSECA, J. F. da
773 $tReproduction in Domestic Animals$gv. 57, n. 4, p. 393-401, Jan. 2022.
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Embrapa Caprinos e Ovinos (CNPC) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Agricultura Digital. Para informações adicionais entre em contato com cnptia.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
20/04/2006 |
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Data da última atualização: |
27/02/2024 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
JARDINE, J. G.; NESHICH, G.; FALCÃO, P. R. K.; YAMAGISHI, M. E. B.; OLIVEIRA, S. R. de M.; MARANGONI, S.; MARTINS, D.; WINCK, F. V.; NOVELLO, J. C. |
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Afiliação: |
JOSE GILBERTO JARDINE, CNPTIA; GORAN NESIC, CNPTIA; PAULA REGINA KUSER FALCAO, CNPTIA; MICHEL EDUARDO BELEZA YAMAGISHI, CNPTIA; STANLEY ROBSON DE MEDEIROS OLIVEIRA, CNPTIA; SÉRGIO MARANGONI, Unicamp; DANIEL MARTINS, Unicamp; FLAVIA V. WINCK, Unicamp; JOSÉ C. NOVELLO, Unicamp. |
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Título: |
Molecular modeling of the protein twitching motility of Xylella fastidiosa. |
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Ano de publicação: |
2005 |
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Fonte/Imprenta: |
In: X-MEETING; INTERNATIONAL CONFERENCE OF THE AB3C, 1., 2005, Caxambu. [Proceedings...]. [S.l.]: Associação Brasileira de Bioinformática e Biologia Computacional, 2005. p. 99. |
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Idioma: |
Inglês |
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Notas: |
Na publicação: Stanley R. M. Oliveira. X-meeting 2005. Presented Posters. |
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Conteúdo: |
In Brazil, citricuture suffers from serious phytopathologic problems that compromise the orange production. For instance, Citrus ariegated Chiorosis (CVC), a disease also known as "amarelinho", caused by Xylella fastidiosa a slow growing gram-negative bacterium that dwells plant xylem and compromises fruit production, among other vegetables. A laboratory consortium in Sao Paulo, Brazil, has published the X. fastidiosa genome, lineage 9a5c, revealing 2.679.305 pairs of bases in the main chromosome and other two plasmids: one with 51.158 and the other with 1.285 pairs of bases. In the total, 2.905 genes were identified. The proteome project of X. fastidiosa revealed over than 800 expressed proteins and identified 112 proteins, some of them potentially involved in the pathogenic processes of this bacterium in citrus. The goal of this research was to develop preliminary studies regarding the tridimensional structure of the protein identified by the proteome PilT, a product of XF1633 gene. Such a protein is associated with adhesion systems and it is a fimbria of type IV, which is supposed to be responsible for the setting of the bacterium in the vascular system of the plant, through its model constructed by homology modeling with the crystallographic structure of a protein of a supposed Víbrio cholerae. |
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Palavras-Chave: |
Estrutura de proteína; Modelagem molecular. |
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Thesagro: |
Proteína; Xylella fastidiosa. |
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Thesaurus NAL: |
Protein structure. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02323nam a2200277 a 4500
001 1008753
005 2024-02-27
008 2005 bl uuuu u00u1 u #d
100 1 $aJARDINE, J. G.
245 $aMolecular modeling of the protein twitching motility of Xylella fastidiosa.$h[electronic resource]
260 $aIn: X-MEETING; INTERNATIONAL CONFERENCE OF THE AB3C, 1., 2005, Caxambu. [Proceedings...]. [S.l.]: Associação Brasileira de Bioinformática e Biologia Computacional, 2005. p. 99.$c2005
500 $aNa publicação: Stanley R. M. Oliveira. X-meeting 2005. Presented Posters.
520 $aIn Brazil, citricuture suffers from serious phytopathologic problems that compromise the orange production. For instance, Citrus ariegated Chiorosis (CVC), a disease also known as "amarelinho", caused by Xylella fastidiosa a slow growing gram-negative bacterium that dwells plant xylem and compromises fruit production, among other vegetables. A laboratory consortium in Sao Paulo, Brazil, has published the X. fastidiosa genome, lineage 9a5c, revealing 2.679.305 pairs of bases in the main chromosome and other two plasmids: one with 51.158 and the other with 1.285 pairs of bases. In the total, 2.905 genes were identified. The proteome project of X. fastidiosa revealed over than 800 expressed proteins and identified 112 proteins, some of them potentially involved in the pathogenic processes of this bacterium in citrus. The goal of this research was to develop preliminary studies regarding the tridimensional structure of the protein identified by the proteome PilT, a product of XF1633 gene. Such a protein is associated with adhesion systems and it is a fimbria of type IV, which is supposed to be responsible for the setting of the bacterium in the vascular system of the plant, through its model constructed by homology modeling with the crystallographic structure of a protein of a supposed Víbrio cholerae.
650 $aProtein structure
650 $aProteína
650 $aXylella fastidiosa
653 $aEstrutura de proteína
653 $aModelagem molecular
700 1 $aNESHICH, G.
700 1 $aFALCÃO, P. R. K.
700 1 $aYAMAGISHI, M. E. B.
700 1 $aOLIVEIRA, S. R. de M.
700 1 $aMARANGONI, S.
700 1 $aMARTINS, D.
700 1 $aWINCK, F. V.
700 1 $aNOVELLO, J. C.
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Embrapa Agricultura Digital (CNPTIA) |
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