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Registro Completo |
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Biblioteca(s): |
Embrapa Algodão; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
08/06/2020 |
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Data da última atualização: |
14/04/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
LOPES, C. M. L.; SUASSUNA, N. D.; CARES, J. E.; GOMES, A. C. M. M.; PERINA, F. J.; NASCIMENTO, G. F.; MENDONÇA, J. S. F.; MOITA, A. W.; CARNEIRO, R. M. D. G. |
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Afiliação: |
C. M. L. LOPES, UNIVERSIDADE DE BRASÍLIA; NELSON DIAS SUASSUNA, CNPA; J. E. CARES, UNIVERSIDADE DE BRASÍLIA; ANA CRISTINA MENESES M GOMES, Cenargen; FABIANO JOSE PERINA, CNPA; G. F. NASCIMENTO; J. S. F. MENDONÇA; ANTONIO WILLIAMS MOITA, CNPH; REGINA MARIA DECHECHI G CARNEIRO, Cenargen. |
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Título: |
Marker-assisted selection in Gossypium spp. for Meloidogyne incognita resistance and histopathological characterization of a near immune line. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Euphytica, v. 216, n. 2, 2020. |
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DOI: |
https://doi.org/10.1007/s10681-020-2554-7 |
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Idioma: |
Inglês |
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Conteúdo: |
ABSTRACT - The root-knot nematode, Meloidogyne incognita, is one of the most important parasites that cause economic losses in the cotton crop. Plant genetic resistance is the most desirable strategy to control this pathogen. Sources of resistance in cotton have been known for several years but only a few resistant commercial varieties have been released. Cotton breeding lines were developed using marker-assisted selection in early generation plants to introgress root-knot resistance genes from two different sources: M-315 or CIR1348. Phenotyping was carried out in greenhouse conditions to validate the molecular markers associated with the resistance genes in the breeding lines and confirmed by genotyping. The markers targeting QTLs from M-315 resistance source were highly efficient in the selection of plants resistant to M. incognita, with all plants expressing a reproduction factor inferior to 0.08. CIR1348 resistance source markers were also very efficient in selecting resistance; however, some segregation events revealed the need for fine mapping of the resistance QTLs. To clarify the resistance mechanisms, present in the germplasm derived from the M-315 resistance source, the resistant line CNPA 17-26 B2RF (triple cross [BRS 368RF 9 M-315] 9 [BRS 430B2RF]) was chosen for histopathological characterization of plant-nematode interaction and compared with the susceptible FiberMax 966 (FM 966). The second-stage juveniles (J2) penetrated equally in both genotypes. In the histopathological study, a strong blue fluorescence was visualized in the tissues around the nematode (hypersensitivity reaction, HR), mainly at the beginning (from 2 to 6 DAI) in the cortex and central cylinder of the resistant plant, indicating accumulation of phenolic compounds in the roots. At 9 DAI, giant cells in the early stage of subdivision next to nematodes were observed in the central cylinder of the resistant plant, and phenolic compounds were also shown around the nematode. At 12–40 DAI these initial cells were completely degraded with the presence of phenolics involving the nematodes and initial giant cells. No fully developed giant cells or mature females were observed, only fourth-stage juveniles (J4s), and males were frequently visualized at 34 DAI. This resistance mechanism characterizes near-immunity, and so no enlarged females and no egg production were observed. In susceptible control, it was possible to visualize feeding sites well developed from 6 to 30 DAI. Females reached maturity at 26 DAI, and eggs were observed at 30 DAI. Our results suggested that the resistance (near-immunity) of the line CNPA 17-26 B2RF was related to early (2–12 DAI) defense responses that totally prevented nematode reproduction. MenosABSTRACT - The root-knot nematode, Meloidogyne incognita, is one of the most important parasites that cause economic losses in the cotton crop. Plant genetic resistance is the most desirable strategy to control this pathogen. Sources of resistance in cotton have been known for several years but only a few resistant commercial varieties have been released. Cotton breeding lines were developed using marker-assisted selection in early generation plants to introgress root-knot resistance genes from two different sources: M-315 or CIR1348. Phenotyping was carried out in greenhouse conditions to validate the molecular markers associated with the resistance genes in the breeding lines and confirmed by genotyping. The markers targeting QTLs from M-315 resistance source were highly efficient in the selection of plants resistant to M. incognita, with all plants expressing a reproduction factor inferior to 0.08. CIR1348 resistance source markers were also very efficient in selecting resistance; however, some segregation events revealed the need for fine mapping of the resistance QTLs. To clarify the resistance mechanisms, present in the germplasm derived from the M-315 resistance source, the resistant line CNPA 17-26 B2RF (triple cross [BRS 368RF 9 M-315] 9 [BRS 430B2RF]) was chosen for histopathological characterization of plant-nematode interaction and compared with the susceptible FiberMax 966 (FM 966). The second-stage juveniles (J2) penetrated equally in both genotypes. In the his... Mostrar Tudo |
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Palavras-Chave: |
G barbadense; Resistance genes; Root-knot nematode. |
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Thesagro: |
Gossypium Hirsutum; Marcador Molecular; Meloidogyne Incognita; Resistência Genética. |
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Thesaurus Nal: |
Genetic resistance; Gossypium barbadense; Hypersensitive response; Phenolic compounds; Root-knot nematodes. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03921naa a2200373 a 4500
001 2139989
005 2023-04-14
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s10681-020-2554-7$2DOI
100 1 $aLOPES, C. M. L.
245 $aMarker-assisted selection in Gossypium spp. for Meloidogyne incognita resistance and histopathological characterization of a near immune line.$h[electronic resource]
260 $c2020
520 $aABSTRACT - The root-knot nematode, Meloidogyne incognita, is one of the most important parasites that cause economic losses in the cotton crop. Plant genetic resistance is the most desirable strategy to control this pathogen. Sources of resistance in cotton have been known for several years but only a few resistant commercial varieties have been released. Cotton breeding lines were developed using marker-assisted selection in early generation plants to introgress root-knot resistance genes from two different sources: M-315 or CIR1348. Phenotyping was carried out in greenhouse conditions to validate the molecular markers associated with the resistance genes in the breeding lines and confirmed by genotyping. The markers targeting QTLs from M-315 resistance source were highly efficient in the selection of plants resistant to M. incognita, with all plants expressing a reproduction factor inferior to 0.08. CIR1348 resistance source markers were also very efficient in selecting resistance; however, some segregation events revealed the need for fine mapping of the resistance QTLs. To clarify the resistance mechanisms, present in the germplasm derived from the M-315 resistance source, the resistant line CNPA 17-26 B2RF (triple cross [BRS 368RF 9 M-315] 9 [BRS 430B2RF]) was chosen for histopathological characterization of plant-nematode interaction and compared with the susceptible FiberMax 966 (FM 966). The second-stage juveniles (J2) penetrated equally in both genotypes. In the histopathological study, a strong blue fluorescence was visualized in the tissues around the nematode (hypersensitivity reaction, HR), mainly at the beginning (from 2 to 6 DAI) in the cortex and central cylinder of the resistant plant, indicating accumulation of phenolic compounds in the roots. At 9 DAI, giant cells in the early stage of subdivision next to nematodes were observed in the central cylinder of the resistant plant, and phenolic compounds were also shown around the nematode. At 12–40 DAI these initial cells were completely degraded with the presence of phenolics involving the nematodes and initial giant cells. No fully developed giant cells or mature females were observed, only fourth-stage juveniles (J4s), and males were frequently visualized at 34 DAI. This resistance mechanism characterizes near-immunity, and so no enlarged females and no egg production were observed. In susceptible control, it was possible to visualize feeding sites well developed from 6 to 30 DAI. Females reached maturity at 26 DAI, and eggs were observed at 30 DAI. Our results suggested that the resistance (near-immunity) of the line CNPA 17-26 B2RF was related to early (2–12 DAI) defense responses that totally prevented nematode reproduction.
650 $aGenetic resistance
650 $aGossypium barbadense
650 $aHypersensitive response
650 $aPhenolic compounds
650 $aRoot-knot nematodes
650 $aGossypium Hirsutum
650 $aMarcador Molecular
650 $aMeloidogyne Incognita
650 $aResistência Genética
653 $aG barbadense
653 $aResistance genes
653 $aRoot-knot nematode
700 1 $aSUASSUNA, N. D.
700 1 $aCARES, J. E.
700 1 $aGOMES, A. C. M. M.
700 1 $aPERINA, F. J.
700 1 $aNASCIMENTO, G. F.
700 1 $aMENDONÇA, J. S. F.
700 1 $aMOITA, A. W.
700 1 $aCARNEIRO, R. M. D. G.
773 $tEuphytica$gv. 216, n. 2, 2020.
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| 104. |  | CARDOSO, M. J.; MELO, F. de B.; BASTOS, E. A. Rendimento de grãos relacionado à adubação nitrogenada e a densidade de plantas na região sul maranhense. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 26; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 10.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 8.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 5., 2004, Lages. Fertbio 2004: anais.... Lages: SBCS, 2004. p. 1-4, 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio-Norte. |
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| 106. | | CARDOSO, M. J.; RIBEIRO, V, Q.; BASTOS, E. A. Rendimento de grãos verdes em cultivares melhoradas e tradicionais de feijão-caupi sob irrigação. In: CONGRESSO NACIONAL DE FEIJÃO-CAUPI, 2., 2009, Belém, PA. Da agricultura de subsistência ao agronegócio: anais. Belém, PA: Embrapa Amazônia Oriental, 2009. p. 787-790. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Meio-Norte. |
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| Registros recuperados : 1,156 | |
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