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 | Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
10/11/2020 |
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Data da última atualização: |
10/11/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA, H. C. de; IBELLI, A. M. G.; GUIMARÃES, S. E. F.; CANTAO, M. E.; PEIXOTO, J. de O.; COUTINHO. L. L.; LEDUR, M. C. |
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Afiliação: |
HANIEL CEDRAZ DE OLIVEIRA, UFL; ADRIANA MERCIA GUARATINI IBELLI, CNPSA; SIMONE ELIZA FACIONI GUIMARÃES, UFL; MAURICIO EGIDIO CANTAO, CNPSA; JANE DE OLIVEIRA PEIXOTO, CNPSA; LUIZ LEHMANN COUTINHO, USP; MONICA CORREA LEDUR, CNPSA. |
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Título: |
RNA-seq reveals downregulated osteochondral genes potentially related totibia bacterial chondronecrosis with osteomyelitis in broilers. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
BMC Genetics, v. 21, n. 58, 2020. |
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DOI: |
https://doi.org/10.1186/s12863-020-00862-2 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract Background: Bacterial chondronecrosis with osteomyelitis (BCO) develops in the growth plate (GP) of the proximal femur and tibia and is initiated by damage to the less mineralized chondrocytes followed by colonization of opportunistic bacteria. This condition affects approximately 1% of all birds housed, being considered one of the major causes of lameness in fast growing broilers. Although several studies have been previously performed aiming to understand its pathogenesis, the molecular mechanisms involved with BCO remains to be elucidated. Therefore, this study aimed to generate a profile of global differential gene expression involved with BCO in the tibia of commercial broilers, through RNA sequencing analysis to identity genes and molecular pathways involved with BCO in chickens. Results: Our data showed 192 differentially expressed (DE) genes: 63 upregulated and 129 downregulated in the GP of the tibia proximal epiphysis of BCO-affected broilers. Using all DE genes, six Biological Processes (BP) were associated with bone development (connective tissue development, cartilage development, skeletal system development, organ morphogenesis, system development and skeletal system morphogenesis). The analyses of the upregulated genes did not indicate any significant BP (FDR < 0.05). However, with the downregulated genes, the same BP were identified when using all DE genes in the analysis, with a total of 26 coding genes explaining BCO in the tibia: ACAN, ALDH1A2, CDH7, CHAD, CHADL, COL11A1, COMP, CSGALNACT1, CYR61, FRZB, GAL3ST1, HAPLN1, IHH, KIF26B, LECT1, LPPR1, PDE6B, RBP4A, SERINC5, SFRP1, SOX8, SOX9, TENM2, THBS1, UCHL1 and WFIKKN2. In addition, seven transcription factors were also associated to BCO: NFATC2, MAFB, HIF1A-ARNT, EWSR1-FLI1, NFIC, TCF3 and NF-KAPPAB. Conclusions: Our data show that osteochondral downregulated genes are potential molecular causes of BCO in broilers, and the bacterial process seems to be, in fact, a secondary condition. Sixteen genes responsible for bone and cartilage formation were downregulated in BCO-affected broilers being strong candidate genes to trigger this disorder. MenosAbstract Background: Bacterial chondronecrosis with osteomyelitis (BCO) develops in the growth plate (GP) of the proximal femur and tibia and is initiated by damage to the less mineralized chondrocytes followed by colonization of opportunistic bacteria. This condition affects approximately 1% of all birds housed, being considered one of the major causes of lameness in fast growing broilers. Although several studies have been previously performed aiming to understand its pathogenesis, the molecular mechanisms involved with BCO remains to be elucidated. Therefore, this study aimed to generate a profile of global differential gene expression involved with BCO in the tibia of commercial broilers, through RNA sequencing analysis to identity genes and molecular pathways involved with BCO in chickens. Results: Our data showed 192 differentially expressed (DE) genes: 63 upregulated and 129 downregulated in the GP of the tibia proximal epiphysis of BCO-affected broilers. Using all DE genes, six Biological Processes (BP) were associated with bone development (connective tissue development, cartilage development, skeletal system development, organ morphogenesis, system development and skeletal system morphogenesis). The analyses of the upregulated genes did not indicate any significant BP (FDR < 0.05). However, with the downregulated genes, the same BP were identified when using all DE genes in the analysis, with a total of 26 coding genes explaining BCO in the tibia: ACAN, ALDH1A2, CD... Mostrar Tudo |
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Palavras-Chave: |
BCO; RNA-Seq. |
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Thesagro: |
Cartilagem; Claudicação; Frango de Corte; Genética Animal; Osteomielite. |
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Thesaurus Nal: |
Animal genetics; Broiler chickens; Cartilage; Gallus gallus; Lameness. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03176naa a2200349 a 4500
001 2126442
005 2020-11-10
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1186/s12863-020-00862-2$2DOI
100 1 $aOLIVEIRA, H. C. de
245 $aRNA-seq reveals downregulated osteochondral genes potentially related totibia bacterial chondronecrosis with osteomyelitis in broilers.$h[electronic resource]
260 $c2020
520 $aAbstract Background: Bacterial chondronecrosis with osteomyelitis (BCO) develops in the growth plate (GP) of the proximal femur and tibia and is initiated by damage to the less mineralized chondrocytes followed by colonization of opportunistic bacteria. This condition affects approximately 1% of all birds housed, being considered one of the major causes of lameness in fast growing broilers. Although several studies have been previously performed aiming to understand its pathogenesis, the molecular mechanisms involved with BCO remains to be elucidated. Therefore, this study aimed to generate a profile of global differential gene expression involved with BCO in the tibia of commercial broilers, through RNA sequencing analysis to identity genes and molecular pathways involved with BCO in chickens. Results: Our data showed 192 differentially expressed (DE) genes: 63 upregulated and 129 downregulated in the GP of the tibia proximal epiphysis of BCO-affected broilers. Using all DE genes, six Biological Processes (BP) were associated with bone development (connective tissue development, cartilage development, skeletal system development, organ morphogenesis, system development and skeletal system morphogenesis). The analyses of the upregulated genes did not indicate any significant BP (FDR < 0.05). However, with the downregulated genes, the same BP were identified when using all DE genes in the analysis, with a total of 26 coding genes explaining BCO in the tibia: ACAN, ALDH1A2, CDH7, CHAD, CHADL, COL11A1, COMP, CSGALNACT1, CYR61, FRZB, GAL3ST1, HAPLN1, IHH, KIF26B, LECT1, LPPR1, PDE6B, RBP4A, SERINC5, SFRP1, SOX8, SOX9, TENM2, THBS1, UCHL1 and WFIKKN2. In addition, seven transcription factors were also associated to BCO: NFATC2, MAFB, HIF1A-ARNT, EWSR1-FLI1, NFIC, TCF3 and NF-KAPPAB. Conclusions: Our data show that osteochondral downregulated genes are potential molecular causes of BCO in broilers, and the bacterial process seems to be, in fact, a secondary condition. Sixteen genes responsible for bone and cartilage formation were downregulated in BCO-affected broilers being strong candidate genes to trigger this disorder.
650 $aAnimal genetics
650 $aBroiler chickens
650 $aCartilage
650 $aGallus gallus
650 $aLameness
650 $aCartilagem
650 $aClaudicação
650 $aFrango de Corte
650 $aGenética Animal
650 $aOsteomielite
653 $aBCO
653 $aRNA-Seq
700 1 $aIBELLI, A. M. G.
700 1 $aGUIMARÃES, S. E. F.
700 1 $aCANTAO, M. E.
700 1 $aPEIXOTO, J. de O.
700 1 $aCOUTINHO. L. L.
700 1 $aLEDUR, M. C.
773 $tBMC Genetics$gv. 21, n. 58, 2020.
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Registro original: |
Embrapa Suínos e Aves (CNPSA) |
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Registro |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Amazônia Oriental. Para informações adicionais entre em contato com cpatu.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Acre; Embrapa Amazônia Oriental. |
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Data corrente: |
11/08/2020 |
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Data da última atualização: |
05/11/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
PANTOJA, K. F. C.; BOARI, A. de J.; MARCHI, B. R. De; REZENDE, J. A. M.; KITAJIMA, E. W.; GONCALVES, R. C.; ASSIS, G. M. L. de; BLAWID, R.; KRAUSE-SAKATE, R. |
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Afiliação: |
Késsia Fátima Cunha Pantoja, UNESP; ALESSANDRA DE JESUS BOARI, CPATU; Bruno Rossitto De Marchi, University of Florida; Jorge Alberto Marques Rezende, ESALQ-USP; Elliot Watanabe Kitajima, ESALQ-USP; RIVADALVE COELHO GONCALVES, CPAF-AC; GISELLE MARIANO LESSA DE ASSIS, CPAF-AC; Rosana Blawid, Universidade Federal Rural de Pernambuco; Renate Krause-Sakate, UNESP. |
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Título: |
Arachis virus Y, a new potyvirid from Brazilian forage peanut (Arachis pintoi). |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Archives of Virology, v. 165, n. 10, p. 2349-2353, Oct. 2020. |
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DOI: |
https://doi.org/10.1007/s00705-020-04742-z |
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Idioma: |
Inglês |
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Conteúdo: |
The complete nucleotide sequence of a new member of the family Potyviridae, which we propose to name ?Arachis virus Y? (ArVY), is reported from forage peanut plants (Arachis pintoi) exhibiting virus-like symptoms. The ArVY positive-sense RNA genome is 9,213 nucleotides long and encodes a polyprotein with 2,947 amino acids that is predicted to be cleaved into 10 mature proteins. The complete single open reading frame (ORF) of ArVY shares 47% and 34% nucleotide and amino acid sequence identity, respectively, with the closest related virus, soybean yellow shoot virus. Electron microscopic analysis revealed elongated viral particles typical of those found in plant cells infected with potyviruses |
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Palavras-Chave: |
Amendoim forrageiro; Arachis virus Y; Cacahuetes forrajeros; Forage peanut; Leguminosas forrajeras; Virus de las plantas. |
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Thesagro: |
Doença de Planta; Leguminosa Forrageira. |
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Thesaurus NAL: |
Arachis pintoi; Forage legumes; Peanuts; Plant viruses; Potyviridae. |
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Categoria do assunto: |
--
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 01843naa a2200385 a 4500
001 2124273
005 2020-11-05
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s00705-020-04742-z$2DOI
100 1 $aPANTOJA, K. F. C.
245 $aArachis virus Y, a new potyvirid from Brazilian forage peanut (Arachis pintoi).$h[electronic resource]
260 $c2020
520 $aThe complete nucleotide sequence of a new member of the family Potyviridae, which we propose to name ?Arachis virus Y? (ArVY), is reported from forage peanut plants (Arachis pintoi) exhibiting virus-like symptoms. The ArVY positive-sense RNA genome is 9,213 nucleotides long and encodes a polyprotein with 2,947 amino acids that is predicted to be cleaved into 10 mature proteins. The complete single open reading frame (ORF) of ArVY shares 47% and 34% nucleotide and amino acid sequence identity, respectively, with the closest related virus, soybean yellow shoot virus. Electron microscopic analysis revealed elongated viral particles typical of those found in plant cells infected with potyviruses
650 $aArachis pintoi
650 $aForage legumes
650 $aPeanuts
650 $aPlant viruses
650 $aPotyviridae
650 $aDoença de Planta
650 $aLeguminosa Forrageira
653 $aAmendoim forrageiro
653 $aArachis virus Y
653 $aCacahuetes forrajeros
653 $aForage peanut
653 $aLeguminosas forrajeras
653 $aVirus de las plantas
700 1 $aBOARI, A. de J.
700 1 $aMARCHI, B. R. De
700 1 $aREZENDE, J. A. M.
700 1 $aKITAJIMA, E. W.
700 1 $aGONCALVES, R. C.
700 1 $aASSIS, G. M. L. de
700 1 $aBLAWID, R.
700 1 $aKRAUSE-SAKATE, R.
773 $tArchives of Virology$gv. 165, n. 10, p. 2349-2353, Oct. 2020.
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