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 | Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
04/11/2020 |
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Data da última atualização: |
04/11/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ESPÍNDOLA, J. P.; BALBINOTT, N.; GRESSLER, L. T.; MACHADO, G.; KLEIN, C. S.; REBELATTO, R.; MARTÍN, C. B. G.; KREUTZ, L. C.; SCHRYVERS, A. B.; FRANDOLOSO, R. |
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Afiliação: |
JÚLIA PIRES ESPÍNDOLA, UPF; NATÁLIA BALBINOTT, UPF; LETÍCIA TREVISAN GRESSLER, UPF; GUSTAVO MACHADO, North Carolina State University; CATIA SILENE KLEIN, CNPSA; RAQUEL REBELATTO, CNPSA; CÉSAR BERNARDO GUTIÉRREZ MARTÍN, University of León; LUIZ CARLOS KREUTZ, UPF; ANTHONY BERNARD SCHRYVERS, University of Calgary; RAFAEL FRANDOLOSO, UPF. |
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Título: |
Molecular serotyping of clinical strains of Haemophilus (Glaesserella) parasuis brings new insights regarding Glassers disease outbreaks in Brazil. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
PeerJ, v. 7, n. e6817, 2019. |
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DOI: |
http://doi.org/10.7717/peerj.6817 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Glässer's disease (GD) is an important infectious disease of swine caused by Haemophilus (Glaesserella) parasuis. Vaccination with inactivated whole cell vaccines is the major approach for prevention of H. parasuis infection worldwide, but the immunity induced is predominantly against the specific polysaccharide capsule. As a consequence, the available vaccines may not induce adequate protection against the field strains, when the capsules present in the vaccine strains are different from those in strains isolated from the farms. Therefore, it is crucial to map H. parasuis serovars associated with regional outbreaks so that appropriate bacterin vaccines can be developed and distributed for prevention of infection. In this study, 459 H. parasuis field strains isolated from different Glässer's disease outbreaks that occurred in 10 different Brazilian States were analyzed for serotype using PCR-based approaches. Surprisingly, non-typeable (NT) strains were the second most prevalent group of field strains and along with serovars 4, 5 and 1 comprised more than 70% of the isolates. A PCRbased approach designed to amplify the entire polysaccharide capsule locus revealed 9 different band patterns in the NT strains, and 75% of the NT strains belonged to three clusters, suggesting that a number of new serovars are responsible for a substantial proportion of disease. These results indicate that commercially available vaccines in Brazil do not cover the most prevalent H. parasuis serovars associated with GD. Disease mapping, Haemophilus parasuis, Typification, Serovars, Vaccines, Brazil Microbiology, Veterinary Medicine MenosAbstract: Glässer's disease (GD) is an important infectious disease of swine caused by Haemophilus (Glaesserella) parasuis. Vaccination with inactivated whole cell vaccines is the major approach for prevention of H. parasuis infection worldwide, but the immunity induced is predominantly against the specific polysaccharide capsule. As a consequence, the available vaccines may not induce adequate protection against the field strains, when the capsules present in the vaccine strains are different from those in strains isolated from the farms. Therefore, it is crucial to map H. parasuis serovars associated with regional outbreaks so that appropriate bacterin vaccines can be developed and distributed for prevention of infection. In this study, 459 H. parasuis field strains isolated from different Glässer's disease outbreaks that occurred in 10 different Brazilian States were analyzed for serotype using PCR-based approaches. Surprisingly, non-typeable (NT) strains were the second most prevalent group of field strains and along with serovars 4, 5 and 1 comprised more than 70% of the isolates. A PCRbased approach designed to amplify the entire polysaccharide capsule locus revealed 9 different band patterns in the NT strains, and 75% of the NT strains belonged to three clusters, suggesting that a number of new serovars are responsible for a substantial proportion of disease. These results indicate that commercially available vaccines in Brazil do not cover the most prevalent H. paras... Mostrar Tudo |
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Palavras-Chave: |
Brasil; Disease mapping; Serovares; Serovars; Tipificação; Typification. |
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Thesagro: |
Microbiologia; Vacina; Veterinária. |
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Thesaurus Nal: |
Brazil; Haemophilus parasuis; Microbiology; Vaccines; Veterinary medicine. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02802naa a2200409 a 4500
001 2126299
005 2020-11-04
008 2019 bl uuuu u00u1 u #d
024 7 $ahttp://doi.org/10.7717/peerj.6817$2DOI
100 1 $aESPÍNDOLA, J. P.
245 $aMolecular serotyping of clinical strains of Haemophilus (Glaesserella) parasuis brings new insights regarding Glassers disease outbreaks in Brazil.$h[electronic resource]
260 $c2019
520 $aAbstract: Glässer's disease (GD) is an important infectious disease of swine caused by Haemophilus (Glaesserella) parasuis. Vaccination with inactivated whole cell vaccines is the major approach for prevention of H. parasuis infection worldwide, but the immunity induced is predominantly against the specific polysaccharide capsule. As a consequence, the available vaccines may not induce adequate protection against the field strains, when the capsules present in the vaccine strains are different from those in strains isolated from the farms. Therefore, it is crucial to map H. parasuis serovars associated with regional outbreaks so that appropriate bacterin vaccines can be developed and distributed for prevention of infection. In this study, 459 H. parasuis field strains isolated from different Glässer's disease outbreaks that occurred in 10 different Brazilian States were analyzed for serotype using PCR-based approaches. Surprisingly, non-typeable (NT) strains were the second most prevalent group of field strains and along with serovars 4, 5 and 1 comprised more than 70% of the isolates. A PCRbased approach designed to amplify the entire polysaccharide capsule locus revealed 9 different band patterns in the NT strains, and 75% of the NT strains belonged to three clusters, suggesting that a number of new serovars are responsible for a substantial proportion of disease. These results indicate that commercially available vaccines in Brazil do not cover the most prevalent H. parasuis serovars associated with GD. Disease mapping, Haemophilus parasuis, Typification, Serovars, Vaccines, Brazil Microbiology, Veterinary Medicine
650 $aBrazil
650 $aHaemophilus parasuis
650 $aMicrobiology
650 $aVaccines
650 $aVeterinary medicine
650 $aMicrobiologia
650 $aVacina
650 $aVeterinária
653 $aBrasil
653 $aDisease mapping
653 $aSerovares
653 $aSerovars
653 $aTipificação
653 $aTypification
700 1 $aBALBINOTT, N.
700 1 $aGRESSLER, L. T.
700 1 $aMACHADO, G.
700 1 $aKLEIN, C. S.
700 1 $aREBELATTO, R.
700 1 $aMARTÍN, C. B. G.
700 1 $aKREUTZ, L. C.
700 1 $aSCHRYVERS, A. B.
700 1 $aFRANDOLOSO, R.
773 $tPeerJ$gv. 7, n. e6817, 2019.
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Registro original: |
Embrapa Suínos e Aves (CNPSA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
10/01/2003 |
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Data da última atualização: |
26/07/2019 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
EL-AOUAR, A. A.; AZOUBEL, P. M.; MURR, F. E. X. |
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Afiliação: |
PATRICIA MOREIRA AZOUBEL, CPATSA. |
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Título: |
Influência do pré-tratamento osmótico na qualidade de mamão Formosa (Carica papaya L.) seco. |
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Ano de publicação: |
2002 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE ENGENHARIA AGRÍCOLA, 31., 2002, Salvador. Anais... Salvador: SBEA/UFBA/Embrapa, 2002. |
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Descrição Física: |
1 CD-ROM. |
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Idioma: |
Português |
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Conteúdo: |
O presente estudo teve como objetivo avaliar a influência do processo de desidratação osmótica como pré-tratamento à secagem de cubos de mamão Formosa (Carica papaya L.). |
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Palavras-Chave: |
Cultivo. |
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Thesagro: |
Desidratação Osmótica; Mamão; Qualidade; Secagem. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CPATSA/25478/1/OPB393.pdf
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Marc: |
LEADER 00817nam a2200205 a 4500
001 1150900
005 2019-07-26
008 2002 bl uuuu u00u1 u #d
100 1 $aEL-AOUAR, A. A.
245 $aInfluência do pré-tratamento osmótico na qualidade de mamão Formosa (Carica papaya L.) seco.
260 $aIn: CONGRESSO BRASILEIRO DE ENGENHARIA AGRÍCOLA, 31., 2002, Salvador. Anais... Salvador: SBEA/UFBA/Embrapa$c2002
300 $c1 CD-ROM.
520 $aO presente estudo teve como objetivo avaliar a influência do processo de desidratação osmótica como pré-tratamento à secagem de cubos de mamão Formosa (Carica papaya L.).
650 $aDesidratação Osmótica
650 $aMamão
650 $aQualidade
650 $aSecagem
653 $aCultivo
700 1 $aAZOUBEL, P. M.
700 1 $aMURR, F. E. X.
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