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Registro Completo |
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Biblioteca(s): |
Embrapa Instrumentação. |
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Data corrente: |
14/10/2020 |
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Data da última atualização: |
12/08/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CÔNSOLO, N. R. B.; SAMUELSSON, L. M.; BARBOSA, L. C. G. S.; MONARETTO, T.; MORAES, T. B.; BUARQUE, V. L. M.; HIGUERA-PADILLA, A. R.; COLNAGO, L. A.; SILVA, S. L.; REIS, M. M.; FONSECA, A. C.; ARAÚJO, C. S. S.; LEITE, B. G. S.; ROQUE, F. A.; ARAÚJO, L. F. |
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Afiliação: |
LUIZ ALBERTO COLNAGO, CNPDIA. |
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Título: |
Characterization of chicken muscle disorders through metabolomics, pathway analysis, and water relaxometry: a pilot study. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Poultry Science, v. 99, n. 11, 2020. |
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Páginas: |
6247-6257 |
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DOI: |
https://doi.org/10.1016/j.psj.2020.06.066 |
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Idioma: |
Inglês |
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Conteúdo: |
Metabolite profiles of chicken breast extracts and water mobility in breasts were studied using proton nuclear magnetic resonance (1H-NMR) spectroscopy and time-domain NMR (TD-NMR) relaxometry, respectively, using normal breast (NB), and wooden breast (WB) and white striping (WS) myopathies in broilers. One thousand eight hundred sixty broilers were raised to commercial standards, receiving the same diets that were formulated as per the different growth stages. At 49 D of age, 200 animals were slaughtered following routine commercial procedures, and at 4 h postmortem, the whole breast (pectoralis major muscle) was removed and visually inspected by an experienced meat inspector who selected NB (without myopathies) and samples with the presence of WS and WB myopathies. Fifteen breasts (5 each of NB, WS, and WB) were analyzed through TD-NMR relaxometry, and samples of approximately 20 g were taken from each breast and frozen at −80°C for metabolite profiling through 1H-NMR spectroscopy. Multivariate statistical analysis was used to evaluate the effect on water relaxometry and metabolite profile in accordance with the presence and type of myopathy in the breast. 1H-NMR data showed that the metabolite profiles in WS and WB breasts were different from each other and from NB. This pilot study shows that myopathies appear to be related to hypoxia, connective tissue deposition, lower mitochondrial function, and greater oxidative stress compared with NB. The longitudinal and transverse relaxation time of the breasts determined by TD-NMR relaxometry was shorter for NB than that for WS and WB, indicating greater water mobility in breasts affected by myopathies. 1H-NMR spectroscopy can be used to differentiate the metabolism of WS, WB, and NB, and TD-NMR has the potential to be a fast, simple, and noninvasive method to distinguish NB from WB and WS. As a practical application, the metabolomic profile as per the occurrence of breast myopathies may be used for a better understanding of these issues, which opens a gap to mitigate the incidence and severity of WS and WB. In addition, the present study brings an opportunity for the development of a new and objective tool to classify the incidence of breast myopathies through TD-NMR relaxometry. MenosMetabolite profiles of chicken breast extracts and water mobility in breasts were studied using proton nuclear magnetic resonance (1H-NMR) spectroscopy and time-domain NMR (TD-NMR) relaxometry, respectively, using normal breast (NB), and wooden breast (WB) and white striping (WS) myopathies in broilers. One thousand eight hundred sixty broilers were raised to commercial standards, receiving the same diets that were formulated as per the different growth stages. At 49 D of age, 200 animals were slaughtered following routine commercial procedures, and at 4 h postmortem, the whole breast (pectoralis major muscle) was removed and visually inspected by an experienced meat inspector who selected NB (without myopathies) and samples with the presence of WS and WB myopathies. Fifteen breasts (5 each of NB, WS, and WB) were analyzed through TD-NMR relaxometry, and samples of approximately 20 g were taken from each breast and frozen at −80°C for metabolite profiling through 1H-NMR spectroscopy. Multivariate statistical analysis was used to evaluate the effect on water relaxometry and metabolite profile in accordance with the presence and type of myopathy in the breast. 1H-NMR data showed that the metabolite profiles in WS and WB breasts were different from each other and from NB. This pilot study shows that myopathies appear to be related to hypoxia, connective tissue deposition, lower mitochondrial function, and greater oxidative stress compared with NB. The longitudinal and transvers... Mostrar Tudo |
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Palavras-Chave: |
Chicken breast myopathy; NMR spectroscopy; TD-NMR relaxometry; Water mobilization. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03360naa a2200361 a 4500
001 2125497
005 2022-08-12
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.psj.2020.06.066$2DOI
100 1 $aCÔNSOLO, N. R. B.
245 $aCharacterization of chicken muscle disorders through metabolomics, pathway analysis, and water relaxometry$ba pilot study.$h[electronic resource]
260 $c2020
300 $a6247-6257
520 $aMetabolite profiles of chicken breast extracts and water mobility in breasts were studied using proton nuclear magnetic resonance (1H-NMR) spectroscopy and time-domain NMR (TD-NMR) relaxometry, respectively, using normal breast (NB), and wooden breast (WB) and white striping (WS) myopathies in broilers. One thousand eight hundred sixty broilers were raised to commercial standards, receiving the same diets that were formulated as per the different growth stages. At 49 D of age, 200 animals were slaughtered following routine commercial procedures, and at 4 h postmortem, the whole breast (pectoralis major muscle) was removed and visually inspected by an experienced meat inspector who selected NB (without myopathies) and samples with the presence of WS and WB myopathies. Fifteen breasts (5 each of NB, WS, and WB) were analyzed through TD-NMR relaxometry, and samples of approximately 20 g were taken from each breast and frozen at −80°C for metabolite profiling through 1H-NMR spectroscopy. Multivariate statistical analysis was used to evaluate the effect on water relaxometry and metabolite profile in accordance with the presence and type of myopathy in the breast. 1H-NMR data showed that the metabolite profiles in WS and WB breasts were different from each other and from NB. This pilot study shows that myopathies appear to be related to hypoxia, connective tissue deposition, lower mitochondrial function, and greater oxidative stress compared with NB. The longitudinal and transverse relaxation time of the breasts determined by TD-NMR relaxometry was shorter for NB than that for WS and WB, indicating greater water mobility in breasts affected by myopathies. 1H-NMR spectroscopy can be used to differentiate the metabolism of WS, WB, and NB, and TD-NMR has the potential to be a fast, simple, and noninvasive method to distinguish NB from WB and WS. As a practical application, the metabolomic profile as per the occurrence of breast myopathies may be used for a better understanding of these issues, which opens a gap to mitigate the incidence and severity of WS and WB. In addition, the present study brings an opportunity for the development of a new and objective tool to classify the incidence of breast myopathies through TD-NMR relaxometry.
653 $aChicken breast myopathy
653 $aNMR spectroscopy
653 $aTD-NMR relaxometry
653 $aWater mobilization
700 1 $aSAMUELSSON, L. M.
700 1 $aBARBOSA, L. C. G. S.
700 1 $aMONARETTO, T.
700 1 $aMORAES, T. B.
700 1 $aBUARQUE, V. L. M.
700 1 $aHIGUERA-PADILLA, A. R.
700 1 $aCOLNAGO, L. A.
700 1 $aSILVA, S. L.
700 1 $aREIS, M. M.
700 1 $aFONSECA, A. C.
700 1 $aARAÚJO, C. S. S.
700 1 $aLEITE, B. G. S.
700 1 $aROQUE, F. A.
700 1 $aARAÚJO, L. F.
773 $tPoultry Science$gv. 99, n. 11, 2020.
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Registro original: |
Embrapa Instrumentação (CNPDIA) |
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| Registros recuperados : 101 | |
| 42. |  | COBUCI, J. A.; COSTA, C. N.; CARDOSO, F. F.; BRACCINI NETO, J.; AMBROSINI, D. P.; MOURA, M. Q. DE. Interação genótipo-ambiente na raça holandesa utilizando modelo de norma de reação. In: SIMPÓSIO BRASILEIRO DE MELHORAMENTO ANIMAL, 10., 2013, Uberaba. Anais... Viçosa: Sociedade Brasileira de Melhoramento Animal, 2013.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite; Embrapa Pecuária Sul. |
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| 43. | | COBUCI, J. A.; EUCLYDES, R. F.; COSTA, C. N.; TORRES, R. de A.; LOPES, P. S.; PEREIRA, C. S. Genetic evaluation for persistency of lactation in Holstein cows using a random regression model. Genetics and Molecular Biology, Ribeirão Preto, v. 30, n. 2, p. 349-355, 2007.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 46. | | COBUCI, J. A.; EUCLYDES, R. F.; LOPES, P. S.; COSTA, C. N.; TORRES, R. de A.; PEREIRA, C. S. Estimation of genetic parameters for test-day milk yield in holstein cows using a random regression model. Genetics and Molecular Biology, v. 28, n. 1, p. 75-83, 2005.| Tipo: Artigo em Periódico Indexado |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 47. | | ALMEIDA, T. P. de; VELHO, M. M. S.; COBUCI, J. A.; COSTA, C. N.; BRACCINI NETO, J. Estimação de parâmetros genéticos para produção de leite, gordura e proteína em vacas da raça Holandesa por meio de análise "tricaracter". In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 44., 2007, Jaboticabal. Anais... Jaboticabal: SBZ, 2007.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 48. | | COBUCI, J. A.; COSTA, C. N.; CARDOSO, F. F.; BRACCINI NETO, J.; AMBROSINI, D. P.; NAZIAZENO, N. A. Estimação da sensibilidade ambiental do mérito genético para produção de leite usando modelo de norma de reação. In: SIMPÓSIO BRASILEIRO DE MELHORAMENTO ANIMAL, 10., 2013, Uberaba. Anais... Viçosa: Sociedade Brasileira de Melhoramento Animal, 2013.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite; Embrapa Pecuária Sul. |
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| 50. | | CARVALHO, N. S.; DALTRO, D. S.; MACHADO, J. D.; CAMARGO, E. V.; PANETTO, J. C. do C.; COBUCI, J. A. Genetic parameters and genetic trends of conformation and management traits in Dairy Gir cattle. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v. 73, n. 4, p. 938-948, 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 51. | | KERN, E. L.; COBUCI, J. A.; COSTA, C. N.; BRACCINI NETO, J.; CAMPOS, G. S.; MCMANUS, C. M. Genetic parameters for longevity measures in Brazilian Holstein cattle using linear and threshold models. Archiv fur Tierzucht, v. 57, n. 33, p. 1-12, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 53. | | BIASSUS, I. de O.; COBUCI, J. A.; COSTA, C. N.; RORATO, P. R. N.; BRACCINI NETO, J.; CARDOSO, L. L. Genetic parameters for production traits in primiparous Holstein cows estimated by random regression models. Revista Brasileira de Zootecnia, v. 40, n. 1, p. 85-94, 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 56. | | CARDOSO, L. L.; BRACCINI NETO, J.; CARDOSO, F. F.; COBUCI, J. A.; BIASSUS, I. de O.; BARCELLOS, J. O. J. Hierarchical Bayesian models for genotype × environment estimates in post-weaning gain of Hereford bovine via reaction norms. Revista Brasileira de Zootecnia, Viçosa, v. 40, n. 2, p. 294-300, 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Pecuária Sul. |
|  |
| 60. | | COBUCI, J. A.; COSTA, C. N.; KERN, E. L.; RIBAS NETO, P.; MARQUES, A.; MACHADO, D. S. Tendencias geneticas das caracteristicas de conformação linear na raça holandesa no Brasil. In: SIMPÓSIO BRASILEIRO DE MELHORAMENTO ANIMAL, 10., 2013, Uberaba. Anais... Viçosa: Sociedade Brasileira de Melhoramento Animal, 2013.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| Registros recuperados : 101 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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