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Registro Completo |
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
16/07/2020 |
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Data da última atualização: |
03/05/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MATEI, J. C.; SOARES, M.; BONATO, A. C. H.; FREITAS, M. P. A. de; HELM, C. V.; MAROLDI, W. V.; MAGALHAES, W. L. E.; HAMINIUK, C. H. Y.; MACIEL, G. M. |
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Afiliação: |
Jéssica C. Matei, UTFPR; Marlene Soares, UTFPR; Aline Cristine H. Bonato, UTFPR; Maria Paula A. de Freitas, UTFPR; CRISTIANE VIEIRA HELM, CNPF; Wedisley V. Maroldi, UFPR; WASHINGTON LUIZ ESTEVES MAGALHAES, CNPF; Charles W. I. Haminiuk, UTFPR; Giselle M. Maciel, UTFPR. |
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Título: |
Enzymatic delignification of sugar cane bagasse and rice husks and its effect in saccharification. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Renewable Energy, v. 157, p. 987-997, Sept. 2020. |
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DOI: |
https://doi.org/10.1016/j.renene.2020.05.028 |
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Idioma: |
Inglês |
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Conteúdo: |
Sugarcane bagasse (SB) and rice husks (RH) are agroindustrial byproducts which can be hydrolyzed to release sugars for the production of valuable bioproducts. However, the exposure of their carbohydrates to the action of hydrolytic enzymes depends on the removal/modification of lignin by a pretreatment. Here we report a biological strategy for pretreatment of SB and RH using a crude enzyme extract rich in laccases to delignification and improvement of reducing sugars yield after saccharification. Enzyme extracts were produced by different species of fungi cultured in a medium with agroindustrial byproducts and other components in static semi-solid condition. The highest yields of laccase production were obtained by Trametes villosa (9467.8 U/L). SB and RH were pretreated with this crude enzyme extract in various conditions and the process optimization resulted in the choice of proper mediators for laccases oxidative action and an increase of more than 10-fold in reducing sugars concentration in the saccharified samples (291 mg/g of sugars were obtained from SB and 193 mg/g from RH) when compared to the control (non pre-treated). Results of MIR-ATR suggested that RH were less susceptible to enzymatic pretreatment than SB and partial removal/modification of lignin was sufficient to improve saccharification. |
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Palavras-Chave: |
Bioetanol; Escarificação; Lacase; Lignin removal; Lignocelulose. |
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Thesaurus Nal: |
Bioethanol; Laccase; Lignocellulose; Saccharification. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02299naa a2200337 a 4500
001 2123881
005 2024-05-03
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.renene.2020.05.028$2DOI
100 1 $aMATEI, J. C.
245 $aEnzymatic delignification of sugar cane bagasse and rice husks and its effect in saccharification.$h[electronic resource]
260 $c2020
520 $aSugarcane bagasse (SB) and rice husks (RH) are agroindustrial byproducts which can be hydrolyzed to release sugars for the production of valuable bioproducts. However, the exposure of their carbohydrates to the action of hydrolytic enzymes depends on the removal/modification of lignin by a pretreatment. Here we report a biological strategy for pretreatment of SB and RH using a crude enzyme extract rich in laccases to delignification and improvement of reducing sugars yield after saccharification. Enzyme extracts were produced by different species of fungi cultured in a medium with agroindustrial byproducts and other components in static semi-solid condition. The highest yields of laccase production were obtained by Trametes villosa (9467.8 U/L). SB and RH were pretreated with this crude enzyme extract in various conditions and the process optimization resulted in the choice of proper mediators for laccases oxidative action and an increase of more than 10-fold in reducing sugars concentration in the saccharified samples (291 mg/g of sugars were obtained from SB and 193 mg/g from RH) when compared to the control (non pre-treated). Results of MIR-ATR suggested that RH were less susceptible to enzymatic pretreatment than SB and partial removal/modification of lignin was sufficient to improve saccharification.
650 $aBioethanol
650 $aLaccase
650 $aLignocellulose
650 $aSaccharification
653 $aBioetanol
653 $aEscarificação
653 $aLacase
653 $aLignin removal
653 $aLignocelulose
700 1 $aSOARES, M.
700 1 $aBONATO, A. C. H.
700 1 $aFREITAS, M. P. A. de
700 1 $aHELM, C. V.
700 1 $aMAROLDI, W. V.
700 1 $aMAGALHAES, W. L. E.
700 1 $aHAMINIUK, C. H. Y.
700 1 $aMACIEL, G. M.
773 $tRenewable Energy$gv. 157, p. 987-997, Sept. 2020.
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| 1. |  | SECCHI, G. A. R.; CASIRAGHI, K. F.; PILATI, R.; FERRARI, M. P.; SILVA, H. D. da. Resíduo do processo de fabricação de papel reciclado - potencial de uso como insumo agrícola e/ou florestal. In: EVENTO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA FLORESTAS, 3., 2004, Colombo. Anais. Colombo: Embrapa Florestas, 2004. 1 CD-ROM. (Embrapa Florestas. Documentos, 102). Resumo.| Tipo: Resumo em Anais de Congresso |
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