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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste; Embrapa Pecuária Sul. |
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Data corrente: |
16/03/2020 |
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Data da última atualização: |
20/04/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
HAEHLING, M. B. V.; CRUVINEL, G. G.; TOSCANO, J. H. B.; GIRALDELO, L. A.; SANTOS, I. B. dos; ESTEVES, S. N.; BENAVIDES, M. V.; BARIONI JUNIOR, W.; NICIURA, S. C. M.; CHAGAS, A. C. de S. |
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Afiliação: |
MAREI BORSCH VON HAEHLING, UNESP; GIOVANNA GABRIELLE CRUVINEL, UNICEP; JOÃO HENRIQUE BARBOSA TOSCANO, UNESP; LUCIANA APARECIDA GIRALDELO, UNICEP; ISABELLA BARBOSA DOS SANTOS, UNESP; SERGIO NOVITA ESTEVES, CPPSE; MAGDA VIEIRA BENAVIDES, CPPSUL; WALDOMIRO BARIONI JUNIOR, CPPSE; SIMONE CRISTINA MEO NICIURA, CPPSE; ANA CAROLINA DE SOUZA CHAGAS, CPPSE. |
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Título: |
Four single nucleotide polymorphisms (SNPs) are associated with resistance and resilience to Haemonchus contortus in Brazilian Morada Nova sheep. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Veterinary Parasitology, v. 279, 2020, 109053. |
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Páginas: |
11 p. |
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ISSN: |
0304-4017 |
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DOI: |
https://doi.org/10.1016/j.vetpar.2020.109053 |
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Idioma: |
Inglês |
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Conteúdo: |
Gastrointestinal nematodes are a major constraint in sheep production. Breeding for resistance has proven to be an effective and feasible approach to address this problem. The use and investigation of genetic markers for resistance traits could accelerate genetic progress and lead to a better understanding of underlying molecular mechanisms. Thus, the aim of this study was to evaluate if five single nucleotide polymorphisms SNPs OAR2_14765360, OAR6_81718546, OAR11_62887032, OAR12_69606944 and OAR15_59871543 are associated with resistance and resilience traits in a flock of the Morada Nova sheep breed. Lambs were submitted to two consecutive parasite challenges by oral infection with 4000 infective larvae L3) of Haemonchus contortus. Fecal egg counts (FEC), packed cell volume (PVC) and body weight were measured every one or two weeks for 42 days in each trial. DNA samples from 287 lambs, 131 ewes and 4 rams were amplified by ARMS-PCR or PCR-RFLP and genotypes were determined. Analysis of variance (ANOVA) was used for association analyses between genotypes and phenotypes. In case of significant association, the allele substitution effect was calculated based on a linear model. OAR2_14765360 and OAR12_69606944 were associated with FEC, and OAR12_69606944 also had significant effects on PCV and weight gain, showing favourable associations of the CC genotype with all evaluated traits. Both OAR6_81718546 and OAR11_62887032 were associated with weight gain, and OAR6_81718546 had an additional effect on PCV. OAR15_59871543 was not polymorphic in the population. OAR6_81718546 and OAR12_69606944 presented significant allele substitution effects of -1.06 ± 0.52 kg for the T allele on final body weight and 0.74 ± 0.32 for the C allele in PCV of the same sampling date, respectively. This is the first report of SNPs associated with gastrointestinal nematode resistance in this sheep breed. Our findings support the existence of quantitatice trait loci (QTL) for resistance and resilience in linkage disequilibrium with the polymorphic SNPs and suggest their future use for explorations of these traits in Morada Nova sheep. MenosGastrointestinal nematodes are a major constraint in sheep production. Breeding for resistance has proven to be an effective and feasible approach to address this problem. The use and investigation of genetic markers for resistance traits could accelerate genetic progress and lead to a better understanding of underlying molecular mechanisms. Thus, the aim of this study was to evaluate if five single nucleotide polymorphisms SNPs OAR2_14765360, OAR6_81718546, OAR11_62887032, OAR12_69606944 and OAR15_59871543 are associated with resistance and resilience traits in a flock of the Morada Nova sheep breed. Lambs were submitted to two consecutive parasite challenges by oral infection with 4000 infective larvae L3) of Haemonchus contortus. Fecal egg counts (FEC), packed cell volume (PVC) and body weight were measured every one or two weeks for 42 days in each trial. DNA samples from 287 lambs, 131 ewes and 4 rams were amplified by ARMS-PCR or PCR-RFLP and genotypes were determined. Analysis of variance (ANOVA) was used for association analyses between genotypes and phenotypes. In case of significant association, the allele substitution effect was calculated based on a linear model. OAR2_14765360 and OAR12_69606944 were associated with FEC, and OAR12_69606944 also had significant effects on PCV and weight gain, showing favourable associations of the CC genotype with all evaluated traits. Both OAR6_81718546 and OAR11_62887032 were associated with weight gain, and OAR6_81718546 had an... Mostrar Tudo |
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Palavras-Chave: |
Host resistance; Morada Nova sheep; Parasite challenge; SNPs. |
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Thesaurus Nal: |
Genetic markers. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 03156naa a2200325 a 4500
001 2121271
005 2020-04-20
008 2020 bl uuuu u00u1 u #d
022 $a0304-4017
024 7 $ahttps://doi.org/10.1016/j.vetpar.2020.109053$2DOI
100 1 $aHAEHLING, M. B. V.
245 $aFour single nucleotide polymorphisms (SNPs) are associated with resistance and resilience to Haemonchus contortus in Brazilian Morada Nova sheep.$h[electronic resource]
260 $c2020
300 $a11 p.
520 $aGastrointestinal nematodes are a major constraint in sheep production. Breeding for resistance has proven to be an effective and feasible approach to address this problem. The use and investigation of genetic markers for resistance traits could accelerate genetic progress and lead to a better understanding of underlying molecular mechanisms. Thus, the aim of this study was to evaluate if five single nucleotide polymorphisms SNPs OAR2_14765360, OAR6_81718546, OAR11_62887032, OAR12_69606944 and OAR15_59871543 are associated with resistance and resilience traits in a flock of the Morada Nova sheep breed. Lambs were submitted to two consecutive parasite challenges by oral infection with 4000 infective larvae L3) of Haemonchus contortus. Fecal egg counts (FEC), packed cell volume (PVC) and body weight were measured every one or two weeks for 42 days in each trial. DNA samples from 287 lambs, 131 ewes and 4 rams were amplified by ARMS-PCR or PCR-RFLP and genotypes were determined. Analysis of variance (ANOVA) was used for association analyses between genotypes and phenotypes. In case of significant association, the allele substitution effect was calculated based on a linear model. OAR2_14765360 and OAR12_69606944 were associated with FEC, and OAR12_69606944 also had significant effects on PCV and weight gain, showing favourable associations of the CC genotype with all evaluated traits. Both OAR6_81718546 and OAR11_62887032 were associated with weight gain, and OAR6_81718546 had an additional effect on PCV. OAR15_59871543 was not polymorphic in the population. OAR6_81718546 and OAR12_69606944 presented significant allele substitution effects of -1.06 ± 0.52 kg for the T allele on final body weight and 0.74 ± 0.32 for the C allele in PCV of the same sampling date, respectively. This is the first report of SNPs associated with gastrointestinal nematode resistance in this sheep breed. Our findings support the existence of quantitatice trait loci (QTL) for resistance and resilience in linkage disequilibrium with the polymorphic SNPs and suggest their future use for explorations of these traits in Morada Nova sheep.
650 $aGenetic markers
653 $aHost resistance
653 $aMorada Nova sheep
653 $aParasite challenge
653 $aSNPs
700 1 $aCRUVINEL, G. G.
700 1 $aTOSCANO, J. H. B.
700 1 $aGIRALDELO, L. A.
700 1 $aSANTOS, I. B. dos
700 1 $aESTEVES, S. N.
700 1 $aBENAVIDES, M. V.
700 1 $aBARIONI JUNIOR, W.
700 1 $aNICIURA, S. C. M.
700 1 $aCHAGAS, A. C. de S.
773 $tVeterinary Parasitology$gv. 279, 2020, 109053.
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Embrapa Pecuária Sudeste (CPPSE) |
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Registro Completo
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
05/12/2018 |
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Data da última atualização: |
05/12/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
MANZKE, N. E.; GOMES, B. K.; XAVIER, E. G.; LIMA, G. J. M. M. de. |
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Afiliação: |
NAIANA EINHARDT MANZKE, UFPel; BRUNA KUHN GOMES, UFPel; EDUARDO GONÇALVES XAVIER, UFPel; GUSTAVO JULIO MELLO M DE LIMA, CNPSA. |
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Título: |
Efficacy of energy supplementation on growth performance and immune response of suckling pigs. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Journal of Animal Science, v. 96, n.8, p. 4723-4730, 2018. |
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DOI: |
10.1093/jas/sky335. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Three studies were performed to determine the effects of oral energy sources on the performance, immune status, and intestinal morphology of piglets. In Exp. 1, 50 litters were selected based on genotype and parity order to determine the optimum amount of supplemental energy for neonatal pigs. They were distributed according to a 5 × 5 Latin square design, in which columns were represented by 5 sows and rows were represented by 5 newborn weight categories. Treatments consisted of 2 oral doses of 0, 2, 4, 8, or 16 mL of rice bran oil (RBO). There was a positive response of RBO on BW with a numerical difference (P = 0.12) and a significant linear effect (P = 0.04) at weaning. In Exp. 2, litters (n = 340) were randomly allotted to a 2 × 2 factorial arrangement of treatments: RBO supplemented either through oral doses (without supplementation or with 2 mL of RBO) or through prestarter feed (without supplementation or with 2% of RBO replacing 2% of soybean oil). Piglets orally receiving RBO showed a numerical increase in BW at weaning (P = 0.101) compared with the control group. Moreover, piglets supplemented by prestarter feed tended to increase feed intake (P = 0.084). In Exp. 3, litters (n = 245) were selected based on genotype and parity order and they were distributed according to a 7 × 7 Latin square design planed in the same way as in Exp. 1. Each neonatal pig within a litter was randomly assigned to 1 of 7 treatments: 1) control (no supplementation), 2) 2 mL of RBO enriched with omega-3 fatty acids, 3) 2.33 mL of pure glycerin, 4) 1.3 mL of soybean oil, 5) 1.4 mL of linseed oil, 6) 1.68 mL of coconut oil, and 7) 2 mL of RBO. Animal performance was analyzed using 2 data sets: all data (average initial BW of 1.479 kg) and low birth weight (LBW) piglet data (?1.220 kg initial BW with average of 0.985 kg). Body weight or BW gain (BWG) were not different among treatments (P > 0.10) when all data were analyzed. However, LBW piglets fed coconut oil tended to increase BW (P = 0.099) during the first week. In conclusion, the oral use of RBO may increase BW at weaning and the supplementation by prestarter feed may enhance the feed intake of piglets. In addition, coconut oil may increase BW of LBW piglets in the first week of life without affecting mortality, immune response, or gut morphology. The routine practice of oral supplementation of energy for newborn pigs could be an important tool in swine production. MenosAbstract: Three studies were performed to determine the effects of oral energy sources on the performance, immune status, and intestinal morphology of piglets. In Exp. 1, 50 litters were selected based on genotype and parity order to determine the optimum amount of supplemental energy for neonatal pigs. They were distributed according to a 5 × 5 Latin square design, in which columns were represented by 5 sows and rows were represented by 5 newborn weight categories. Treatments consisted of 2 oral doses of 0, 2, 4, 8, or 16 mL of rice bran oil (RBO). There was a positive response of RBO on BW with a numerical difference (P = 0.12) and a significant linear effect (P = 0.04) at weaning. In Exp. 2, litters (n = 340) were randomly allotted to a 2 × 2 factorial arrangement of treatments: RBO supplemented either through oral doses (without supplementation or with 2 mL of RBO) or through prestarter feed (without supplementation or with 2% of RBO replacing 2% of soybean oil). Piglets orally receiving RBO showed a numerical increase in BW at weaning (P = 0.101) compared with the control group. Moreover, piglets supplemented by prestarter feed tended to increase feed intake (P = 0.084). In Exp. 3, litters (n = 245) were selected based on genotype and parity order and they were distributed according to a 7 × 7 Latin square design planed in the same way as in Exp. 1. Each neonatal pig within a litter was randomly assigned to 1 of 7 treatments: 1) control (no supplementation), 2) 2 mL of ... Mostrar Tudo |
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Palavras-Chave: |
Leitão recém-nascido; Neonatal; Óleo de côco; Óleo de farelo de arroz; Óleo de linhaça; Óleo de soja. |
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Thesagro: |
Dieta; Glicerina; Leitão; Nutrição Animal; Óleo Vegetal. |
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Thesaurus NAL: |
Animal nutrition; Coconut oil; Linseed oil; Neonates; Piglet feeding; Rice bran oil; Soybean oil. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03537naa a2200385 a 4500
001 2100816
005 2018-12-05
008 2018 bl uuuu u00u1 u #d
024 7 $a10.1093/jas/sky335.$2DOI
100 1 $aMANZKE, N. E.
245 $aEfficacy of energy supplementation on growth performance and immune response of suckling pigs.$h[electronic resource]
260 $c2018
520 $aAbstract: Three studies were performed to determine the effects of oral energy sources on the performance, immune status, and intestinal morphology of piglets. In Exp. 1, 50 litters were selected based on genotype and parity order to determine the optimum amount of supplemental energy for neonatal pigs. They were distributed according to a 5 × 5 Latin square design, in which columns were represented by 5 sows and rows were represented by 5 newborn weight categories. Treatments consisted of 2 oral doses of 0, 2, 4, 8, or 16 mL of rice bran oil (RBO). There was a positive response of RBO on BW with a numerical difference (P = 0.12) and a significant linear effect (P = 0.04) at weaning. In Exp. 2, litters (n = 340) were randomly allotted to a 2 × 2 factorial arrangement of treatments: RBO supplemented either through oral doses (without supplementation or with 2 mL of RBO) or through prestarter feed (without supplementation or with 2% of RBO replacing 2% of soybean oil). Piglets orally receiving RBO showed a numerical increase in BW at weaning (P = 0.101) compared with the control group. Moreover, piglets supplemented by prestarter feed tended to increase feed intake (P = 0.084). In Exp. 3, litters (n = 245) were selected based on genotype and parity order and they were distributed according to a 7 × 7 Latin square design planed in the same way as in Exp. 1. Each neonatal pig within a litter was randomly assigned to 1 of 7 treatments: 1) control (no supplementation), 2) 2 mL of RBO enriched with omega-3 fatty acids, 3) 2.33 mL of pure glycerin, 4) 1.3 mL of soybean oil, 5) 1.4 mL of linseed oil, 6) 1.68 mL of coconut oil, and 7) 2 mL of RBO. Animal performance was analyzed using 2 data sets: all data (average initial BW of 1.479 kg) and low birth weight (LBW) piglet data (?1.220 kg initial BW with average of 0.985 kg). Body weight or BW gain (BWG) were not different among treatments (P > 0.10) when all data were analyzed. However, LBW piglets fed coconut oil tended to increase BW (P = 0.099) during the first week. In conclusion, the oral use of RBO may increase BW at weaning and the supplementation by prestarter feed may enhance the feed intake of piglets. In addition, coconut oil may increase BW of LBW piglets in the first week of life without affecting mortality, immune response, or gut morphology. The routine practice of oral supplementation of energy for newborn pigs could be an important tool in swine production.
650 $aAnimal nutrition
650 $aCoconut oil
650 $aLinseed oil
650 $aNeonates
650 $aPiglet feeding
650 $aRice bran oil
650 $aSoybean oil
650 $aDieta
650 $aGlicerina
650 $aLeitão
650 $aNutrição Animal
650 $aÓleo Vegetal
653 $aLeitão recém-nascido
653 $aNeonatal
653 $aÓleo de côco
653 $aÓleo de farelo de arroz
653 $aÓleo de linhaça
653 $aÓleo de soja
700 1 $aGOMES, B. K.
700 1 $aXAVIER, E. G.
700 1 $aLIMA, G. J. M. M. de
773 $tJournal of Animal Science$gv. 96, n.8, p. 4723-4730, 2018.
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