| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Florestas. Para informações adicionais entre em contato com cnpf.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Florestas. |
|
Data corrente: |
13/11/2019 |
|
Data da última atualização: |
13/03/2020 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
BETTENCOURT, G. M. de F.; SOCCOL, C. R.; GIOVANELLA, T. S.; FRANCISCON, L.; KESTRING, D. R.; GERHARDT, I. R.; DEGENHARDT-GOLDBACH, J. |
|
Afiliação: |
Gisela Manuela de França Bettencourt, UFPR; Carlos Ricardo Soccol, UFPR; Thais Salete Giovanella, UFPR; LUZIANE FRANCISCON, CNPF; DAIANE RIGONI, CNPF; ISABEL RODRIGUES GERHARDT, CNPTIA; JULIANA DEGENHARDT GOLDBACH, CNPF. |
|
Título: |
Agrobacterium tumefaciens-mediated transformation of Eucalyptus urophylla clone BRS07-01. |
|
Ano de publicação: |
2020 |
|
Fonte/Imprenta: |
Journal of Forestry Research, v. 31, n. 2, p. 507-519, Apr. 2020. |
|
DOI: |
10.1007/s11676-018-0777-4 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Abstract Genetic transformation is becoming routine for engineering specific traits in important clones of recalcitrant species such as Eucalyptus; however, the efficiency is still low for most species, so many researchers still use seeds instead of clones as initial explants. This work aimed to develop a genetic transformation protocol, based on a highly efficient in vitro organogenesis protocol, for an Eucalyptus urophylla clone selected in our breeding program. Plant growth regulators were evaluated for indirect organogenesis and rooting. In a two-step protocol, the combination of callus induction media supplemented with 0.5 lM thidiazuron + 0.5 lM naphthaleneacetic acid (NAA) and shoot induction media supplemented with 5.0 lM benzylaminopurine + 1.0 lM NAA allowed up to 85.6% shoot formation with more shoots per explants when compared with other concentrations. Transgenic plants expressing the uidA gene were obtained using Agrobacterium tumefaciens and selected for kanamycin resistance. A RAPD analysis was used to check for somaclonal variation. In tests using 11 RAPD primers, we did not observe somaclonal variation in the in vitro stages evaluated. |
|
Palavras-Chave: |
Plant growth regulators; Regeneração in vitro; Reguladores de crescimento de plantas; Transformação genética. |
|
Thesagro: |
Eucalipto; Eucalyptus Urophylla. |
|
Thesaurus Nal: |
Genetic transformation; In vitro regeneration; Somaclonal variation. |
|
Categoria do assunto: |
G Melhoramento Genético |
|
Marc: |
LEADER 02181naa a2200313 a 4500
001 2114265
005 2020-03-13
008 2020 bl uuuu u00u1 u #d
024 7 $a10.1007/s11676-018-0777-4$2DOI
100 1 $aBETTENCOURT, G. M. de F.
245 $aAgrobacterium tumefaciens-mediated transformation of Eucalyptus urophylla clone BRS07-01.$h[electronic resource]
260 $c2020
520 $aAbstract Genetic transformation is becoming routine for engineering specific traits in important clones of recalcitrant species such as Eucalyptus; however, the efficiency is still low for most species, so many researchers still use seeds instead of clones as initial explants. This work aimed to develop a genetic transformation protocol, based on a highly efficient in vitro organogenesis protocol, for an Eucalyptus urophylla clone selected in our breeding program. Plant growth regulators were evaluated for indirect organogenesis and rooting. In a two-step protocol, the combination of callus induction media supplemented with 0.5 lM thidiazuron + 0.5 lM naphthaleneacetic acid (NAA) and shoot induction media supplemented with 5.0 lM benzylaminopurine + 1.0 lM NAA allowed up to 85.6% shoot formation with more shoots per explants when compared with other concentrations. Transgenic plants expressing the uidA gene were obtained using Agrobacterium tumefaciens and selected for kanamycin resistance. A RAPD analysis was used to check for somaclonal variation. In tests using 11 RAPD primers, we did not observe somaclonal variation in the in vitro stages evaluated.
650 $aGenetic transformation
650 $aIn vitro regeneration
650 $aSomaclonal variation
650 $aEucalipto
650 $aEucalyptus Urophylla
653 $aPlant growth regulators
653 $aRegeneração in vitro
653 $aReguladores de crescimento de plantas
653 $aTransformação genética
700 1 $aSOCCOL, C. R.
700 1 $aGIOVANELLA, T. S.
700 1 $aFRANCISCON, L.
700 1 $aKESTRING, D. R.
700 1 $aGERHARDT, I. R.
700 1 $aDEGENHARDT-GOLDBACH, J.
773 $tJournal of Forestry Research$gv. 31, n. 2, p. 507-519, Apr. 2020.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Florestas (CNPF) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 2 | |
| 2. |  | BARROS, J. D.; ARCOVERDE, G. F. B.; CARVALHO, A. C. F. de; VILA, A. J. T.; FAUSTINO, A. B.; MELO, A. A. de; VERAS, A. L.; MARTINS, C. S. G.; MELO, D. M. A. de; SANTOS, D. C. dos; VERDE, D. C. A. L.; TROVÃO, D. M. de B. M.; SILVA, E. S. da; SILVA, E. D. da C.; BARBOSA, E.; SILVA, F. G. C. da; ARAÚJO, H. F. P. de; MAIA, I. C.; SA, I. B.; RODRIGUES, J. de D.; CUNHA, J. E.; COSTA, J. D. da; CARVALHO, J. V.; LIMA, L. de C.; SANTOS, L. M. dos; ARAÚJO, L. N. de; COELHO, L. F. M.; VEIGA, M. E. B. da; ARAÚJO, M. C.; OLIVEIRA, M. G. de; MELO, M. G. P. de; GADELHA, M. A. da M. B.; SANTOS, M. C. dos; LIRA FILHO, P. de B.; LIMA, R. B. de; BATISTA, R. D.; OLIVEIRA, T. C. T. de; BEZERRA, U. A.; SOUZA, V. N. de. Processo participativo sobre degradação da terra em regiões do Semiárido brasileiro. São José dos Campos: SP, INPE, 2023. 53 p. il. color sid.inpe.br/mtc-m21d/2023/04.18.16.48-RPQ| Biblioteca(s): Embrapa Semiárido. |
|    |
| Registros recuperados : 2 | |
|
| Expressão de busca inválida. Verifique!!! |
|
|
